Accurate Detection of Methicillin-Resistant Staphylococcus aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR

Supplemental material

  • Supplemental file 1 -

    Tables S1 (Bacterial strains used in this study), S2 (MIR of MRSA N315 under different concentrations), S3 (MIR of mixtures of MSSA and MR-CoNS under different concentrations), and S4 (Detection results of dulpex ddPCR and duplex qPCR for 104 nasal specimens) and Fig. S1 (1D plots of duplex ddPCR assay under different annealing temperatures), S2 (Activity changes of ClyH after cooling on ice), S3 (2D plots of duplex ddPCR for detection of mixture of MSSA 91118 and MR-CoNS under different concentrations), S4 (2D plots of duplex ddPCR for detection of mixture of MSSA 91118 and MR-CoNS under different concentrations), and S5 (2D plots of duplex ddPCR for detection of mixture of MSSA 91118 and MR-CoNS under different concentrations)

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