Fig. S1. Proteolytic degradation of GbpC111-522 displayed on SDS-PAGE gel. Fig. S2. Cartoon describing the orientation of the V-region of GbpC and AgI/II. Fig. S3. Scatchard and Hill plots from ITC data with 9,400, 38,800, and 70,800 Da dextran. Fig. S4. Reverse ITC of GbpC with dextran, and ITC for interactions of AgI/II and SspB with dextran. Fig. S5. Sensorgram for GbpC interaction with immobilized SRCR1 in the presence/absence of calcium. Fig. S6. Effect of 5,000 Da dextran on the adherence of AgI/II to immobilized SRCR1. Fig. S7. Ligplots for interactions of Ca2+ in native GbpC and GbpC-BGC complex structures. Fig. S8. Ligplots for interactions of BGC1 and BGC2 in the Ca2+ binding site. Fig. S9. (a) Sensorgrams for interaction of GbpC with SRCR1 or SRCR123 and deglycosylated SRCR1 or SRCR123. (b) Sensorgram for GbpC interaction with immobilized SAG. Table S1. Affinities assessed from dextran inhibition experiments. Table S2. B-factors for residues of loop region 410 to 418.
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