Cytoplasmic Relocalization and Colocalization with Viroplasms of Host Cell Proteins, and Their Role in Rotavirus Infection

Supplemental material

• Supplemental file 1 -

  Table S1 (NSP5-interacting proteins identified by mass spectrometry.)

  Table S2 (Cellular proteins interacting with NSP2 identified by mass spectrometry.)

  Table S3 (Time-dependent fold change in the level of cellular proteins in the cytoplasm and the nucleus during the course of rotavirus infection.)

  Table S4 (Source, host, dilution, and catalogue numbers of different antibodies.)

  Fig. S1 (Comparative analysis of the total steady-state levels of cellular proteins in virus-infected and uninfected MA104 cells.)

  Fig. S2 (Predominant nuclear localization of hnRNPs and ARE-BPs in serum-grown control MA104 cells.)

  Fig. S3 (Time-dependent detection of viroplasm formation after infection of MA104 cells with RRV.)

  Fig. S4 (Z-stack analysis of colocalization of viral and nuclear proteins in the VS.)

  Fig. S5 (Colocalization of fluorescent protein-tagged host proteins with viroplasms.)

  Fig. S6 (Analysis of the influence of ectopic expression of IS2-NSP5, SA11-NSP2, and IS2-VP6 in HEK293T cells on the cytoplasmic relocalization of hnRNPs and ARE-BPs.)

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