LTB₄ and 5-oxo-ETE from extracellular vesicles stimulate neutrophils in granulomatosis with polyangiitis

Supplemental Data

• Supplemental Figure S1 (.pdf, 135 KB) - Gating strategy for flow cytometry analysis of EV coated aldehyde beads.
• , Supplemental Tables S1 and S2 (.pdf, 68 KB) - Positive and negative ionization MS/MS analysis details
• Details on the analytical procedure (.pdf, 86 KB) - Measurement of oxylipins cargo in extracellular vesicles
• Supplemental Figures S2-S4 (.pdf, 185 KB) - HPLC separated LTB4
• Supplemental Figures S2-S4 (.pdf, 120 KB) - HPLC separated for 5-oxo-ETE
• Supplemental Figures S2-S4 (.pdf, 185 KB) - HPLC separated 5-HETE
• Supplemental Table S3 (.pdf, 89 KB) - Selected characteristics of the study participants
• Supplemental Figure. S5 (.pdf, 202 KB) - Binding of PKH 67 tracking dye-labeled EV to human neutrophils
• supplemental Figure S6 (.pdf, 91 KB) - Targeted eicosanoids lipidomics of extracellular vesicles
• Supplemental Figure S7 (.pdf, 127 KB) - ROS production and dsDNA release by human neutrophils