Table 1. Suppression assay summary.
| Percent suppression using the percentage of proliferating cells (%) | Percent suppression using the Divison Index (%) | |||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Ratio Treg:Tresp | Number Treg/Tconv | CCR5low Treg | CCR5high Treg | Tconv | CCR5low Treg | CCR5high Treg | Tconv |
| A | 1:1 | 10 000 | 28.5 | 32.1 | 0.0 | 82.0 | 87.4 | 37.1 |
| B | 1:1 | 5 000 | 75.1 | 81.4 | −16.0 | 96.1 | 95.9 | 44.9 |
| C | 1:1 | 10 000 | 58.8 | 83.6 | 16.7 | 75.2 | 88.1 | 46.6 |
| D | 1:1 | 10 000 | 6.7 | 46.6 | −1.3 | 28.8 | 61.3 | −2.7 |
| C | 1:2 | 5 000 | 45.3 | 77.7 | 11.6 | 85.1 | 91.8 | 39.1 |
| D | 1:2 | 5 000 | 8.7 | 34.3 | 16.8 | 37.8 | 74.8 | −34.2 |
| E | 1:2 | 5 000 | 55.1 | 72.2 | −0.7 | 85.8 | 92.8 | 4.1 |
| C | 1:4 | 2 500 | 29.7 | 60.9 | −2.7 | 61.5 | 85.1 | 16.8 |
| D | 1:4 | 2 500 | 0.0 | 6.0 | −9.4 | 3.6 | 28.8 | 0.9 |
| Pooled data for ratios 1:1 and 1:2 Median (interquartile range) | 45.3 (18.6-56.9) | 72.2 (40.4-79.6) | −0.7 (−2.1-14.1) | 82.0 (56.5-85.5) | 88.1 (81.1-92.3) | 37.1 (69.2-42.0) | ||
| Percent suppression method | CCR5low Treg versus Tconv | CCR5high Treg versus Tconv | CCR5low Treg versus CCR5high Treg |
|---|---|---|---|
| Percentage of proliferating cells | 0.043 | 0.018 | 0.018 |
| Division index | 0.018 | 0.018 | 0.028 |
The percent suppression of Tresp proliferation is shown for CRC-isolated CCR5low Treg, CCR5high Treg and Tconv at different Treg:Tresp ratios. The number of Treg/Tconv represents the number of sort events given by the MoFlo XDP High-Speed Cell Sorter, rather than the absolute number of sorted viable cells.
The differences in percent suppression for ratios of 1:1 and 1:2 between different cell populations were tested for statistical significance using the Wilcoxon signed-rank test (P-values shown below).