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. Author manuscript; available in PMC: 2017 May 22.
Published in final edited form as: Hypertension. 2017 Apr 17;69(6):1207–1216. doi: 10.1161/HYPERTENSIONAHA.117.09156

Figure 4. NPNT is a Wnt target gene and produces aldosterone via Wnt.

Figure 4

(a). Constitutive Wnt activation (ΔN-Bcat) induces NPNT mRNA expression, while constitutive Wnt repression (ΔN-TCF4) decreased NPNT expression compared to vector control (n=3).

(b). Wnt transcriptional complex TCF/LEF activity decreased in NPNT-overexpressed and increased in NPNT-silenced samples, as measured by firefly/renilla luciferase assay (n=6, n=4).

(c). Wnt inhibitor LGK-974 attenuated the increase in protein-normalized aldosterone production with addition of NPNT protein, compared to negative controls bovine serum albumin (BSA) and fibronectin (n=3).

Bars represent mean expression per group±s.e.m. Statistical analyses were conducted by Student's t-test. *P<0.05; **P<0.005; ***P<0.0005