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. Author manuscript; available in PMC: 2017 May 22.
Published in final edited form as: Hypertension. 2017 Apr 17;69(6):1207–1216. doi: 10.1161/HYPERTENSIONAHA.117.09156

Figure 5. NPNT is pro-adhesive in normal adrenal and APA cells, but anti-adhesive in H295R.

Figure 5

(a). NPNT is pro-adhesive in i. HEK, ii. Normal primary adrenal, iii. ZF-like APA, iv. ZG-like APA, but anti-adhesive in v. H295R, as demonstrated by wells pre-coated with PBS, BSA, NPNT or laminin, and measured by Xcelligence cell impedance as cell index over time (4h for cell-lines, 10h for primary adrenal cells) (n=2 for ZF-like APA and ZG-like APA, n=4 for the rest).

(b). NPNT is anti-adhesive in H295R cells, as confirmed by Hoechst fluorescent stain assay measuring % maximum fluorescence as a representation of number of cells adhered to well with increasing concentrations of BSA, NPNT and laminin precoating (n=3 for each protein at each concentration).

Bars represent mean values per group±s.e.m.