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Evolving a GASP-enabled strain (ZK819) in prolonged stationary phase and the emergence of small colony forms. (A) Representative picture of small colonies as seen on an LB agar plate spread with a 29-day-old culture of ZK819. (B) Daily colony count of 11 independent replicates of E. coli ZK819 for 30 days in prolonged stationary phase. First recorded emergences of small colonies in different replicate lines are marked by red arrows.
