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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: J Neurosci. 2009 Jul 22;29(29):9292–9300. doi: 10.1523/JNEUROSCI.6063-08.2009

Proteinase-activated receptors in the nucleus of the solitary tract: evidence for glial-neural interactions in autonomic control of the stomach

Gerlinda E Hermann 1, Montina J Van Meter 1, Jennifer C Rood 2, Richard C Rogers 1
PMCID: PMC2773000  NIHMSID: NIHMS136262  PMID: 19625519

Abstract

Bleeding head injury is associated with gastric stasis; a symptom of collapse of autonomic control of the gut described by Cushing around 1932. Recent work suggests that the proteinase thrombin, produced secondary to bleeding, may be the root cause. Results from our in vivo physiological studies show that fourth ventricular injection of PAR1 agonists, as well as thrombin itself, produced significant reductions in gastric transit in the awake rat. We expected that the PAR1 effect to inhibit gastric transit was the result of direct action on vago-vagal reflex circuitry in the dorsal medulla. Surprisingly, our immunohistochemical studies demonstrated that PAR1 receptors are localized exclusively to the astrocytes and not the neurons in the nucleus of the solitary tract [NST; principal locus integrating visceral afferent input and part of the gastric vago-vagal reflex control circuitry]. Our in vitro calcium imaging studies of hindbrain slices revealed that PAR1 activation initially causes a dramatic increase in astrocytic calcium, followed seconds later by an increase in calcium signal in NST neurons. The neuronal effect, but not the astrocytic effect, of PAR1 activation was eliminated by glutamate receptor antagonism. TTX did not eliminate the effects of PAR1 activation on either glia or neurons. Thus, we propose that glia are the primary CNS sensors for PAR agonists and that the response of these glial cells drives the activity of adjacent [e.g., NST] neurons. These results show, for the first time, that changes in autonomic control can be directly signaled by glial detection of local chemical stimuli.

Keywords: vagal, PAR1, astrocytes, nucleus of solitary tract, gastric transit, brainstem

Introduction

Gastrointestinal [GI] stasis, gastric hypersecretion, gastric dilation, anorexia, nausea, and emesis are observed routinely in patients that have suffered bleeding head injuries (Young et al., 1992; Lu et al., 1997). This severe visceral malaise is also observed following traumatic bodily injury, burns, and surgical procedures that are associated with extensive bleeding such as lung, heart and liver transplantation (Berkowitz et al., 1995; Sodhi et al., 2002). There is a high degree of gastric feeding intolerance demonstrated by these patients, thus, they require aggressive total parenteral nutrition and, occasionally, invasive GI surgical intervention (Akindipe et al., 2000) for the maintenance of a positive energy and nitrogen balance. Additionally, there is a significant risk of aspiration that accompanies severe and prolonged gastric stasis such that intensive management of the airway is also required (Berkowitz et al., 1995). Clearly, failure to regulate gastric function is a significant cause of post-trauma morbidity and mortality.

The association of gastric stasis with severe trauma, surgery, and burn injury has been axiomatic in the clinical literature for more than 70 years [e.g., “Cushing’s ulcer”, (Young et al., 1992; Lu et al., 1997).]. Head trauma is associated with elevated intracranial pressure [ICP] as well as the abovementioned gastric complications. However, clinical studies that have attempted to correlate ICP with the degree of gastric dysfunction have not been successful (Larson et al., 1984). Experimental studies of ICP effects on gastric function are also inconsistent, unless extremely high pressures are used. That is, although application of 80mm Hg intracerebroventricular pressure in rodents will produce a mild gastric stasis, this degree of intracranial pressure is four times greater than that observed in humans with “high” ICP after head trauma (Larson et al., 1984; Garrick et al., 1988). Furthermore, it is clear that high ICPs can not be the factor responsible for the gastric dysfunction that is associated with systemic bleeding, burns or trauma that occur outside the CNS. However, all of these injuries, including traumatic burn injury, are significant generators of thrombin (Lavrentieva et al., 2008).

An unusual class of receptor called protease-activated receptor [PAR] has been localized in rather high density to the medullary hindbrain (Weinstein et al., 1995); specifically in the dorsal vagal complex (DVC), an area responsible for vagal control of the stomach (Rogers et al., 2005). PARs represent a unique subclass of Gq-coupled receptors (Macfarlane et al., 2001) which are activated by proteinase cleavage of the extracellular N-terminus of the receptor by serine proteases [e.g., thrombin]. This "tethered ligand", a specific amino acid sequence on the tether [e.g., hexapeptide sequence SFLLRN-NH2] acts as the receptor agonist. The protease thrombin [produced as a consequence of bleeding, burns, or trauma] could act directly upon PAR receptors within the medullary hindbrain.

Therefore, we propose that this interaction of thrombin [or its untethered ligand SFLLRN-NH2] with PAR1 receptors in the DVC causes the disruption of vagal control of the stomach and is manifested as the gastric stasis, nausea, emesis and anorexia that has been associated with bleeding, burns, or trauma. We will examine the potential mechanism by which thrombin or a specific PAR1 agonist modulates autonomic control of digestion by monitoring gastric emptying, examining the distribution of PAR1 in the DVC, and by observing changes in calcium signaling in DVC cells in response to PAR1 receptor activation.

Methods

A total of 41 Long-Evans rats [200–400 g; either sex], obtained from the breeding colony located at Pennington Biomedical Research Center, were used in these studies. All animals were maintained in a room with a 12:12 hour light-dark cycle with constant temperature and humidity, and given food and water ad libitum. All experimental protocols were performed according to the guidelines set forth by the National Institutes of Health and were approved by the Institutional Animal Care and Use Committees at the Pennington Biomedical Research Center.

Fourth ventricle effects of thrombin and PAR1 agonist on gastric transit in awake rats

Surgery

Rats [N = 15] were anesthetized with pentobarbital [50mg/kg, ip; Nembutal, Abbott Labs, Chicago] and placed in a stereotaxic frame in the skull-level position. Using aseptic methods, the skull was exposed via midline incision and a 1mm hole made on the midline of the temporal plate 11.3mm caudal to Bregma. Two additional holes were made in the skull 4mm rostral to the first and 2 mm lateral to the midline; number 80 stainless screws were inserted into these holes as a support for the guide cannula. A 5mm long, 25ga stainless steel guide cannula [Plastics One, Inc., Roanoke, VA] was then inserted and cemented to the skull and the support screws with dental acrylic. An obturator [5mm length] was used to close the cannula until the injection of experimental agents immediately prior to the in vivo physiological studies. The scalp incision was sutured closed and animals were allowed to recover from this surgery for 5–7days before physiological studies were begun.

C13-octanoate gastric transit

Gastric emptying rate in an awake, freely moving animal can be monitored by determining the rate of appearance of 13C in respired CO2 that had been ingested as 13C- tagged sodium octanoate (Ghoos et al., 1993; Ghoos et al., 2002; Schoonjans et al., 2002). The appearance of 13C in respired CO2 is indicative of the transit of the carbohydrate meal from the stomach to, and absorption by, the duodenum. One of the significant advantages of this method is that animals may be used multiple times in a repeated measures design with intervening saline injections serving as controls. Thus, each animal can serve as its own control. Previous methods that used methyl red/cellulose meals were highly invasive as they required gavage and transit was determined by direct observation [ex vivo] of the remaining colored contents of the stomach. Thus, only one time data point per animal was possible. Further, this 13C method captures the entire time course of the transit event [samples taken at 15min intervals]. Useful parameters include Tlag [which corresponds with the time at which rate of excretion of 13CO2 is maximum] and T1/2 [gastric half-emptying time; i.e., time when half of the label that is to be excreted has been excreted] and the gastric emptying coefficient [GEC; a global index of rate of emptying]. All three parameters can be derived through non-linear regression methods [for mathematical curve fitting formulas and derivations, refer to Ghoos (Ghoos et al., 1993)]. However, Tlag may also be identified simply as the time that corresponds to the peak amount of 13CO2 produced per hour from the raw data plot and T1/2 is the time interval at which half of the total cumulative fractions of the administered dose has been excreted [i.e., half of the total area under the curve].

Animals permanently fitted with fourth ventricular guide cannulae [as described above and by Grill (Grill et al., 2002)] were adapted to handling and to spending several hours in the metabolic chamber before the beginning of the experimental sequence. Rats were food deprived overnight. In the morning, they were placed in the Plexiglas chambers; stable levels of CO2 [between 0.2 to 1.0%] were established. The animals were presented with a 1g test meal of waffle that had been labeled with 10mg 13C- tagged sodium octanoate. Immediately upon consuming the doped waffle, rats received a fourth ventricular microinjection [2uL] of saline or agonist. Fourth ventricular injections were made by replacing the obturator with a 9mm long, 31ga injection cannula through the guide cannula. The injection cannula was connected by PE10 tubing to a Hamilton syringe that contained either vehicle control [sterile saline] or test solutions [i.e., 4U thrombin; 1 or 10nmoles of SFLLRN-NH2 dissolved in sterile saline].

For the sake of comparison to illustrate the effect of vagally-mediated, motility-inhibiting anorectics (Flanagan et al., 1992) on this transit model, separate groups of animals were subjected to intraperitoneal injections of cholecystokinin [CCK; 5ug/kg] or lithium chloride [LiCl; 40mg/kg; isotonic for IP injection; volume = 0.6ml/100g body weight] and compared to the transit patterns after intraperitoneal saline injections.

Appearance of 13C in respired CO2 was monitored via 10cc air samples of the chambers taken at 15min intervals over the subsequent 4 hours and analyzed via stable isotope mass spectrometry [Finnigan Delta XP Isotope Ratio Mass Spectrometer; Thermo Scientific, Waltham, MA]; similar to the method described by Schoonjans (Schoonjans et al., 2002). GEC, Tlag and T1/2 are extracted by non-linear regression from the raw data plots of percent 13CO2 in the expired gas as described by Ghoos (Ghoos et al., 1993).

Statistical Analysis of 13C transit data

Gastric transit parameters [GEC, Tlag and T1/2] were derived for each animal under each experimental condition. Each appropriate saline control [either ip or 4V] preceded the corresponding experimental condition by at least two days to allow for recovery from the overnight fast. Each animal served as its own control and comparisons were made as paired T-tests for each parameter under the five conditions; differences were considered statistically significant when P < 0.05 [Figure 1].

Figure 1. C13-octanoate gastric transit.

Figure 1

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Gastric emptying rate in an awake, freely moving animal can be monitored by determining the rate of appearance of 13C in respired CO2 that had been ingested as 13C- tagged sodium octanoate doped meal. 13C in respired CO2 is indicative of the transit of the carbohydrate meal from the stomach to the duodenum. This 13C method captures the entire time course of the transit event [samples taken at 15min intervals] and is displayed in the right hand graphs. Specific parameters of this transit can be extracted for comparisons: Tlag corresponds with the time at which rate of excretion of 13CO2 is maximal, T1/2 is the gastric half-emptying time [time when half of the label that is to be excreted has been excreted] and the gastric emptying coefficient [GEC; a global index of rate of emptying]. Each animal served as its own control. That is, the first session the animal receives the control injection [saline either ip or 4V, as appropriate]; the next session the animal receives the test injection. Therefore, paired t-test comparisons could be made between the saline control and the agonist condition for each animal. Examples of such paired gastric transit experiments of individual animals are seen in the right hand graphs. In the left hand column are group averages for the gastric transit parameters under each condition; statistical comparisons were only made within each condition group. Microinjection of thrombin [4U] or the PAR1-selective agonist SFLLRN-NH2 [1 or 10nmoles] into the fourth ventricle caused a significant reduction in gastric transit as measured by Tlag and T1/2 [B, C, and D]. Thrombin and 10nmole SFLLRN-NH2 also slowed the overall GEC compared to their respective saline controls [C and D]. For the sake of comparison, the dynamics of gastric transit suppression caused by intraperitoneal injection of either CCK-8 or LiCl are shown in [A] and [E], respectively. * = p < 0.05

Distribution and phenotypic identification of DVC cells possessing PAR1

Double immunohistochemical [IHC] staining techniques were used to locate and phenotypically identify PAR1 receptor-bearing elements in the CNS. Identification of these PAR1-positive components was investigated by IHC staining for neurons [i.e., neuron-specific nuclear antibody [NeuN]; (Wolf et al., 1996) versus glia [i.e., glial fibrillary acidic protein, GFAP; (Debus et al., 1983; Hermann et al., 2001)] as described earlier for the chemokine receptor CXCR4 (Hermann et al., 2008).

Animals [N = 12] were deeply anesthetized with urethane [ethyl carbamate, Sigma, St Louis, MO, USA; 1 g/kg, i.p.] and transcardially perfused with phosphate-buffered saline [PBS] followed by 4% paraformaldehyde in PBS. Brains were removed; cryoprotected overnight in a 30% sucrose solution; cut into 40-micron sections on a freezing microtome. The free-floating histological sections were placed in PBS prior to dual immunofluorescence staining. [Note: between each incubation step, the sections were triple-rinsed in PBS; 5min per rinse.] The tissue sections were pretreated with 1% sodium borohydride to remove residual aldehyde fixative. The blocking step consisted of 10% normal donkey serum, 0.3% Triton X-100 and PBS for 2h. Sections were incubated [overnight at room temperature on a shaker table] in a cocktail of dual primary antibodies.

Tissue sections were placed in a cocktail of secondary antibodies for 2hr incubation. Following incubation with biotin-SP-donkey anti-goat secondary antibody, the sections were placed in Vector Elite avidin-biotin-peroxidase complex [Vector Laboratories] for 1h. Tissue sections were subjected to tyramide signal amplification to enhance the demonstration of the PAR1 receptor. The sections were mounted on Plus(c) slides [Fisher] and coverslipped with ProLongGold [Invitrogen]. Sections were viewed on a Nikon E800 fluorescence microscope and images collected with a Zeiss Axiocam [Figure 2, Figure 3, Figure 4 and Figure 5].

Figure 2. IHC identification of location of PAR1-positive cells in the dorsal vagal complex [DVC].

Figure 2

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[A] Low power view of the distribution of GFAP-ir astrocytes in the dorsal vagal complex. Note that the medial solitary nucleus [mNST] contains substantially more astrocytes than surrounding areas, including the dorsal motor nucleus of the vagus [DMN] and the solitary tract [ST]. AP = area postrema, bv = blood vessel, cc = central canal, fg = fasciculus gracilus

[B–D] Single astrocytic fiber [GFAP-ir = red] in DMN possesses PAR1 receptors [PAR1-ir = green] as seen in merged image [D]. The DMN is practically devoid of astrocytic profiles, though the few that are there show co-localization with PAR1-ir.

[E–G] Single GFAP profile within the fascicles of the ST; also possesses PAR1 receptors as seen in the merged image [G]. This area shows the same pattern as seen in the DMN.

[H–J] Dense astrocytic label [GFAP-ir] in the mNST is extensively double labeled for PAR1-ir. Scale bars: A = 200 microns ; B,C,D = 20microns ; E,F,G = 15microns ; H,I,J = 30microns.

Figure 3. IHC identification of PAR1-positive cell types in NST.

Figure 3

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Double IHC studies demonstrated that PAR-ir [middle panels] is co-localized on astrocytes [GFAP-ir; top panels] but not on neurons [NeuN-ir; bottom panels] in the NST. Examples of co-localization are shown at arrows. bv = blood vessels. Scale bars: top = 20micron; bottom = 100micron.

Figure 4. Live cell imaging identification of neuronal versus glial characteristics.

Figure 4

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[A] Hindbrain astrocytes [outlined in white] tend to be smaller, more irregular in shape, and have higher resting calcium levels relative to neurons [outlined in yellow].

[B] Response of individual neuron or astrocyte to 30sec exposure to 200uM glutamate

Figure 5. Response patterns of astrocytes and neurons to SFLLRN-NH2.

Figure 5

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[A] CAG, the Ca++ reporter dye, is taken up by both astrocytes (a) and neuronal cells (n).

[B] SR101 astrocyte-specific vital dye staining.

[C] Same field as both [A] and [B]; demonstration of in vitro glial and neuronal identification. A–C double fluorescence confocal images were taken through 509 and 607nm bandpass filters.

[D] Responses of the same identified glial and neuron cells in A–C following 60sec bath exposure to 20uM SFLLRN-NH2. Note delay in onset and peak of neuronal response relative to glial response. Raw 500nm long-pass filtered image frames on the right illustrate relative calcium levels in the astrocyte and neuron [outlined in dotted circles] at the approximately the peak of the SFLLRN-NH2 response [indicated by dashed vertical line through the response curves] and at rest.

Live Cell Imaging

Direct Injection of Calcium Green Dextran and SR101 into the solitary nucleus

Animals [N = 14] were anesthetized with urethane and placed in stereotaxic frame. Using aseptic technique, the occipital plate of the skull was removed to expose the medullary brainstem. A micropipette [30micron tip diameter; filled with 1% Calcium Green 1 AM [CAG; Invitrogen], 0.3% sulforhodamine 101 [SR101; Sigma Chemical] and 10% pluronic-DMSO [F-127, Invitrogen; in pH 7.2 tris-PBS buffer] was directed toward the medial solitary nucleus using a stereotaxic carrier. Four nanoinjections [40nL each] of the CAG-SR101 solution were made unilaterally into the NST at the level of calamus and 0.2, 0.4 and 0.6mm anterior to calamus; all at a depth of 300micron below surface. [Note that the NST was specifically targeted as a result of the preliminary IHC data which showed that both astrocytes and PAR1-ir are concentrated in the NST.] This injection pattern labeled the ipsilateral medial NST. CAG, a calcium reporter dye, labels both neurons and glia while SR101 labels only astrocytes (Nimmerjahn et al., 2004) but does not interfere with CAG fluorescence. This method made it possible to easily discriminate astrocytes from neurons in a slice preparation. After 30 minutes, the anesthetized rat was decapitated and the brainstem was quickly harvested. The caudal brainstem was glued to a ceramic block and placed in cold [~4°C] carbogenated [95% O2; 5% CO2] cutting solution [see recipe below]. Coronal sections [300micron thick] were cut through the medulla using a Vibratome equipped with a sapphire knife. Slices were incubated in a carbogenated normal Krebs at 29°C for at least 30min prior to transfer to the imaging chamber.

Fluorescence imaging

Individual medullary slices were transferred to a temperature regulated perfusion chamber [Bioptechs, Inc, Butler, PA] and held in place with a harp-type pressure foot. Imaging was performed with a Nikon E600FN upright microscope equipped with a PerkinElmer CSU21 Ultraview spinning disk confocal illuminator and heated, water-immersion objectives. The recording chamber was continuously perfused at a rate of 2ml/min with carbogenated Krebs warmed to 33°C. Identification of SR101-labeled astrocytes was done using a 568nm excitation, 607nm emission filter set. CAG fluorescence and relative intracellular calcium levels were visualized when switching to the 488nm excitation/ 509nm emission filter. Solenoid valves were used to switch between the normal bathing solution and the experimental solutions [see recipes below].

In Vitro Drugs and Solutions

The cutting solution contained [in mM]: 110 choline chloride, 25 NaHCO3, 2.5 KCl, 7 MgSO4-7H2O, 1.25 NaH2PO4; 10 glucose; 0.5 CaCl2-2H2O; bubbled with 95% O2/ 5% CO2 during the entire cutting process. Normal Krebs contained [in mM]: 124 NaCl, 25 NaHCO3, 3.0 KCl, 1 MgSO4-7H2O, 1.5 NaH2PO4; 10 glucose; 1.5 CaCl2-2H2O; bubbled with 95% O2/ 5% CO2, continuously; osmolality was 300 ± 10mOsm; pH = 7.3. As needed for the different experimental conditions, the following drugs were added to the Normal Krebs:

  • glutamate [excitatory amino acid, 200uM; Sigma]

  • SFLLRN-NH2 [PAR1-selective agonist, 20uM; Peptides International]

  • glutamate antagonist cocktail [consisting of 1mM kynurenate – an AMPA/kainate antagonist; 50uM MK-801 – an NMDA antagonist; and 300uM AP3 – a non-specific metabotropic glutamate receptor antagonist; – (Paton et al., 1991; Virgili et al., 1995)]

  • U73122 [10uM, phospholipase C antagonist - blocks the PAR1 phospholipase C transduction step; (Hwang et al., 2005)]

  • tetrodoxin [TTX; 1uM; blocks voltage gated sodium channels and action-potential dependent transmitter release; (Lee and Ruben, 2008)]

In vitro Calcium Signal Analysis

Brainstem slices were challenged with 200uM glutamate for 30sec, relative intracellular calcium levels [i.e., [Ca++]i] were monitored continuously from 10sec before glutamate exposure through 2min after to visualize the viability and basal responsiveness of cells [both neural and glial] to glutamate. For the next 10min, the slices were exposed to normal Kreb’s perfusion for washout; [Ca++]i was not monitored during this time. Next, the slices were exposed to SFLLRN-NH2 [20uM in normal Kreb’s for 60sec]; [Ca++]i of neurons and glia was monitored before and after SFLLRN-NH2 exposure. Alternatively, during the 10min normal washout period, slices were exposed to either a glutamate antagonist cocktail [kynurenate, MK-801, plus AP3] or to a phospholipase C antagonist [U73122] before the slices were exposed to SFLLRN-NH2. Relative changes in [Ca++]i in response to glutamate or SFLLRN-NH2, were quantified as changes in fluorescence: (ΔF/F)%, where F is the fluorescence intensity within an area of interest [e.g., the outline of an SR101-labeled astrocyte or a non-SR101-labeled NST neuron] prior to stimulation and ΔF is the change from this value resulting from cellular activity (Helmchen, 2000). Background fluorescence [i.e., non-involved areas in same field] was subtracted from both Δ̣F and F. The following parameters were extracted from the calcium signal data from each cell examined under normal versus blockade conditions:

  • magnitude of response [peak (ΔF/F)%]

  • time of onset of calcium response and

  • time to peak calcium response

These parameters were determined for each region of interest [ROI; individual glia or neuronal cells] and could be compared statistically. Specifically, magnitudes of responses to SFLLRN-NH2 by glia were compared to that elicited in neurons as unpaired t-tests. Data regarding response time of onset or time to peak were also compared as unpaired t-tests. Modulation of response magnitudes to SFLLRN-NH2 by either glia or neurons as a consequence of glutamate antagonists, PLC inhibitors, or neuronal transmission blocker [TTX] were compared in one way analyses of variance across each cell type. P values < 0.05 were considered significantly significant; Dunnett’s post test comparisons were performed [Figure 7].

Figure 7. Schematic representation of possible relationship between afferents, astrocytes, and NST neurons.

Figure 7

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The results from these experiments suggest the hypothesis that local and circulating agents may act directly on glia to powerfully regulate the synaptic strength of hindbrain autonomic reflexes and visceral afferent circuits such as those involved in feeding behavior. Additionally, it is hypothesized that vagal afferent input can modulate the sensitivity of glial chemosensation via glutamatergic input. Such an intertwined system would allow for maximal range of feedback and feedforward regulation of reflexes.

RESULTS

Thrombin and SFLLRN-NH2 effects on gastric transit in the neurally intact, awake rat

Microinjection of thrombin [4U; low end of the dose range (4–20U) used to model stroke. Note that 50uL blood clot produces ~15U of thrombin (Hua et al., 2007)] or the PAR1-selective agonist SFLLRN-NH2 [1 or 10nmoles] into the fourth ventricle, overlying the DVC, caused a significant reduction in gastric transit as measured by Tlag and T1/2 [Figure 1]. The degree of transit suppression is similar to that produced by intraperitoneal injection of CCK-8 [5ug/kg; produces a significant suppression of gastric motility as well as feeding behavior (Flanagan et al., 1992)]. In addition, both thrombin and the higher dose of SFLLRN-NH2 [10nmoles] also slowed the overall gastric emptying coefficient relative their respective saline controls.

The gastric transit effects of ICV thrombin may be potentially confounded by psychomotor difficulties in that the rats tended to appear catatonic for approximately an hour after the intraventricular injection. [Intracranial injection of thrombin has been reported to produce seizure activity in rats (Lee et al., 1997; Kelly, 2008).] While microinjections of the hexapeptide ligand, SFLLRN-NH2, suppressed gastric transit parameters, it was not accompanied by seizure-like effects.

As a radical point of comparison, intraperitoneal injection of LiCl [commonly used to produce visceral aversion (Coil et al., 1978)] produced a profound suppression of transit that lasted longer than the duration of the experiment [Figure 1E]. Since there was no discernible transit in the LiCl case, the regression model failed to extract any estimates for the overall gastric emptying coefficient.

Immunohistochemical identification of cell types possessing PAR1 receptors

Our physiological observations of slowed gastric transit in response to ICV administration of PAR agonists would suggest that this is a neurally-mediated activity ultimately due to the modulation of vagal efferent projections to the gut (Rogers et al., 2005). However, much to our surprise, our double IHC studies of the dorsal vagal complex showed PAR1-positive staining overlaps that of GFAP-positive [astrocytes] cells and fibers [Figure 2 and Figure 3] in the NST. In contrast, there was no obvious overlap of PAR1-positive staining with cells immunopositive for NeuN [i.e., neurons]. As Figure 2 illustrates, the medial NST [mNST] contains a dense concentration of astrocytes relative to the adjacent areas, including the DMN and solitary tract [ST]. Together these results suggest that PAR1 agonists act primarily on astrocytes in the NST, as opposed to vagal afferent fibers in the ST, NST neurons or DMN neurons. With this in mind, we focused our live cell imaging efforts on the mNST.

Live cell calcium imaging of the effects of PAR1 agonist action on hindbrain slices

In the in vitro slice preparation, glia and neurons are clearly discriminable by basic morphology, uptake of SR101 label, and calcium influx response to 200uM glutamate [Figure 4, Figure 5]. Even without SR101 counterstaining, astrocyte cell bodies appear smaller, more angular and brighter in appearance, due to their higher resting levels of cytoplasmic calcium, than neurons (Kang and Nedergaard, 2000). Further, calcium responsiveness to 200uM glutamate discriminates NST neurons from glia [Figure 4]. NST neurons produce an immediate increase in intracellular calcium [average (ΔF/F)% = 46% ± 5.5] to the 30sec exposure to 200uM glutamate challenge, while NST astrocytes produce little or no increase in intracellular calcium [average (ΔF/F)% = 9% ± 2.4] to this brief pulse exposure to relatively low concentration glutamate. A cocktail of glutamate antagonists [kynurenate, MK801, and AP3 meant to block AMPA, NMDA, and metabotropic receptors, respectively] was required to eliminate the neuronal response to 200uM glutamate [data not shown].

The PAR1 agonist SFLLRN-NH2 produced a relatively rapid and dramatic increase in glial cytoplasmic calcium with an overlay of oscillation [Figure 5 and Figure 6]. This characteristic glial response was followed [~5sec later] by a delayed, monotonic increase in calcium signal in NST neurons [Figure 5D; Table 2]. Both the onset and time of peak response to SFLLRN-NH2 was significantly faster than that seen in the neurons [Table 2; unpaired t-tests; * = p<0.001]. These observations suggest that glia are the primary responders that, in turn, activate the neurons during exposure to SFLLRN-NH2.

Figure 6. Response patterns of astrocytes and neurons to SFLLRN-NH2 during blockade.

Figure 6

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Glia and neurons have different response patterns [e.g., time of onset and magnitude] to SFLLRN-NH2. Magnitude of response to SFLLRN-NH2 is nearly 3X greater in glia than neurons [left panel; # unpaired t-test, p<0.001].These differences are further delineated in the presence of a glutamate antagonist cocktail [kynurenate, MK 801, plus AP3] which abolishes the neuronal “response” to SFLLRN-NH2, while the glial response appears only slightly muted [bar graph and raw data trace]. When pretreated with the PLC inhibitor, U73122, for 10min prior to the SFLLRN-NH2 challenge, PLC inhibition abolished the SFLLRN-NH2 response in both glia and, in turn, neurons. Lastly, when SFLLRN-NH2 is applied in the presence of TTX, the fundamental response to SFLLRN-NH2 in both glia and neurons is still seen. Unlike the glutamate blockade condition where the neuronal response was essentially abolished, under TTX conditions [which should be blocking action potential dependent transmitter release], the neuronal response is not effected. These results provide evidence that the glia are the primary targets of SFLLRN-NH2; that neurons are subsequently activated by glutamate release from activated glia; and that glia may be under tonic vagal afferent influence. [*ANOVA glial response, Dunnett’s post test p<0.001; +ANOVA neuronal response, Dunnett’s post test p<0.001].

Table 2.

Timing parameters of response to 20uM SFLLRN-NH2

glia (n = 104) neurons (n =51)
onset of response (sec; ave ± SEM) 65 ± 1.0 71 ± 1.5*
time of peak response (sec) 84 ± 1.8 102 ± 2.6*
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The magnitude of response to SFLLRN-NH2 is nearly 3-fold greater in glia than neurons [Figure 5 and Figure 6; Table 3]. Pretreatment with the glutamate antagonist cocktail essentially eliminated the apparent delayed effect of SFLLRN-NH2 on neurons; while the glial response was reduced only by approximately one-third [Figure 6]. In contrast, pretreatment with the phospholipase C [PLC] antagonist, U73122, blocked the SFLLRN-NH2 effect on both glia and neurons [Figure 6 top panel; Table 3]. Lastly, while perfusion of the slice with SFLLRN-NH2 in the presence of TTX [Figure 6] caused a slight, but statistically significant decrease [not unlike that seen with glutamate blockade] in the magnitude of the glial response, there was no change in the neuronal response. This would suggest that the glia are potentially under the tonic influence of neurons or vagal afferents.

Table 3.

Magnitude of response to 20uM SFLLRN-NH2

control glutamate
blockade		 PLC
blockade		 TTX
glia 110 ± 5.8 *73 ± 5.1 *5.8 ± 1.0 *64 ± 8.1 *ANOVA glial response F3,101 = 34,
[ave ± SEM] P <0.0001; Dunnett’s post test
n = 55 n = 13 n = 14 n = 23 p<0.001
neurons 40 ± 3.2 +5.8 ± 1.1 +6.5 ± 1.3 33 ± 8.0 +ANOVA neuronal response F3,65 =
n = 29 n = 14 n = 17 n = 9 30, P <0.0001; Dunnett’s post test
p<0.001
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Taken together these data suggest that not only are glia the primary targets of SFLLRN-NH2 but that astrocytes probably use glutamate to subsequently activate the adjacent NST neurons. Given that the PAR1 transduction mechanism has been principally linked to PLC (Macfarlane et al., 2001; Hwang et al., 2005), our observation that PLC blockade eliminates the activation of both astrocytes and neurons in response to SFLLRN-NH2 reinforces our interpretation that glia are the primary targets of SFLLRN-NH2 activation which would normally be passed to surrounding NST neurons via glutamate gliotransmission.

DISCUSSION

Patients with traumatic brain injury or bleeding bodily trauma are chronically unable to maintain nutritional homeostasis (Akindipe et al., 2000). Historically, more interest has been directed to the cachectic, hypermetabolic state that accompanies such injuries. A less appreciated cause of morbidity is the chronic gastric stasis. In the case of closed head injury, an increase in intracranial pressure had been thought to be the main culprit. However, ventricular pressures recorded from head injury patients do not correlate with the occurrence of gastric stasis (Larson et al., 1984). The correlation between bleeding trauma and gastrointestinal autonomic dysfunction, while clear, has been difficult to explain. With these factors in mind, it seemed more likely that mechanisms associated with bleeding, rather than CNS pressure, could have a direct effect on the autonomic control of the gut. Thrombin, a protease generated as a consequence of the clotting cascade, can have devastating effects on neuronal excitability in the hippocampus and these PAR1 - mediated effects are probably responsible for seizure activity that accompanies the appearance or delivery of the protease in the CNS (Kelly, 2008). The mechanism connecting thrombin with neuron hyper-excitability is not well understood. However, proteases are involved in long-term potentiation and activation of glutamatergic mechanisms has been associated with thrombin-induced seizures (Tomimatsu et al., 2002). In any event, the present study suggests that a thrombin-mediated derangement of autonomic control operating through PAR1 receptors at the level of the hindbrain astrocyte is a good possibility and may be responsible for the collapse in autonomic control of digestion that occurs secondary to traumatic injury.

The dorsal medulla contains the neural circuitry essential to the vagal reflex regulation of the stomach (Rogers et al., 2005). NST neurons that receive visceral afferent inputs are also essential to the regulation of feeding behavior, especially satiation. Earlier work by Weinstein (Weinstein et al., 1995) demonstrated that mRNA for PAR1 receptors is densely localized to the NST and dorsal motor nucleus of the vagus; hindbrain structures containing critical gastric vago-vagal reflex circuitry. However, this earlier work utilized a radiation-based in situ hybridization method which showed the general histological location of PAR1 receptor expression but not the phenotype of the cells involved.

We show that fourth ventricular application of SFLLRN-NH2 and thrombin causes a significant reduction in gastric transit. This implies that activation of PAR1 receptors, ultimately, activates solitary nucleus neurons involved in vagal reflex control of the gut. Given that activation of NST neurons has been shown to cause a strong, vagally-mediated suppression of gastric tone, motility and transit (Rogers et al., 2005), it had been our assumption that the relevant PAR1 receptors were localized to NST neurons. However, our immunohistochemical results suggested that these receptors are, in fact, localized to astrocytes and not neurons within this nucleus. This finding is supported by the calcium imaging study which showed that, as a population, astrocytes are activated by SFLLRN-NH2 seconds earlier than neurons in the area. Further, increases in NST neuronal calcium after exposure to SFLLRN-NH2 are essentially eliminated by a glutamate antagonist cocktail while NST glia still respond to SFLLRN-NH2 [though their response is muted relative to the response seen under normal conditions]. This muted response to SFLLRN-NH2 by glia after glutamate antagonist cocktail suggests that glia in the NST may be tonically exposed to glutamate. One potential source of glutamate in the NST-slice preparation is spontaneous release from vagal afferent terminals (Lawrence, 1995). If correct, then it is possible that glial sensitivity to agonist stimulation could be modulated by activity in vagal afferents. Indeed, TTX had the same effect on the glial response to SFLLRN-NH2 as complete block of glutamate transmission [Figure 6]; an approximate 30% reduction in responsiveness to the hexapeptide. TTX had no effect on the responses of neurons to SFLLRN-NH2. Together, these results show that SFLLRN-NH2 primarily activates astrocytes in the NST and these astrocytes, in turn, cause a glutamate mediated increase in cytoplasmic calcium in NST neurons. Our observations strongly suggest that the glia are potentially under the tonic influence of afferent neuronal inputs. If this mechanism is correct, then vagal afferent stimulation should cause calcium waves in NST astrocytes as well as neurons. We have collected preliminary evidence that this is the case [Rogers and Hermann, Society for Neurosciences Meeting 2008; Abstract #284.15]. Finally, both glial and neural responses to SFLLRN-NH2 are blocked by the PLC antagonist U73122. This would be expected given that the PAR1 transduction mechanism is principally linked to PLC (Macfarlane et al., 2001; Hwang et al., 2005). Clearly, if the glial response to SFLLRN-NH2 is blocked by PLC antagonism, then the subsequent activation of neurons by glia is also lost.

The classic neurophysiological role for the astrocyte in the control of CNS function has been passive and supportive. In this view, the astrocyte controls the extracellular nutrient, metabolite, and ionic environment for the neuron, while also working to dispose of released neurotransmitters and their break-down products (Volterra and Meldolesi, 2005). While astrocytes certainly perform these important functions, recent neurophysiological studies in mixed neural-glial culture suggest that astrocytes are critical regulators of neuronal excitability and synaptic efficiency (Haydon and Carmignoto, 2006). Astrocytes possess a broad array of receptors for neurotransmitters, hormones, cytokines, and other agonist peptides. Agonist inputs to these astrocytic receptors can cause dramatic increases in cytoplasmic calcium (Haydon and Carmignoto, 2006), which, in turn, is coupled to astrocytic release of a variety of neuroactive substances, especially glutamate.

Agonist induced calcium signals in astrocytes can cause the delayed activation of adjacent neurons (Parpura and Haydon, 2000). Neurophysiological studies in neuronal-glial co-cultures suggest that the delayed signal generated in neurons may be the result of a calcium-induced exocytosis of glutamate from the glia (Parpura and Haydon, 2000). Depending on the circuitry under study, astrocytic glutamate release can affect neuronal excitability through action on metabotropic, NMDA, and/or AMPA receptors (Haydon and Carmignoto, 2006). Again, depending on the specifics of the circuitry, these astrocyte-glutamate mediated effects can increase or decrease the post-synaptic sensitivity of neurons to presynaptic input (Fellin et al., 2006). While there is strong evidence for astrocytic glutamate release exerting dominating effects on neuronal function, recent studies show that similar effects can also be produced by astrocytic release of other agonists including serine, ATP, or nitric oxide (Haydon and Carmignoto, 2006). These candidate "gliotransmitters" will certainly affect the excitability of the NST.

Astrocytes are also implicated in the presynaptic regulation of afferent neuronal signal traffic. Glial release of ATP can act at a variety of pre-synaptic purinergic receptors to produce a range of circuit-specific effects. In hippocampal culture models, glial release of ATP and its subsequent conversion to adenosine by an ectoenzyme, ultimately results in a presynaptic, A1 receptor-mediated inhibition of excitatory transmission (Newman, 2003). ATP release from glia might also be expected to have potent effects to increase neuronal cytoplasmic calcium and, ultimately, glutamate release from vagal afferent terminals that possess the ATP-gated P2X3 calcium channels (Jin et al., 2004; Shigetomi and Kato, 2004; Rogers et al., 2006b; Rogers et al., 2006a). Though intriguing, this proposition has not been tested directly. It is also possible that astrocytic release of glutamate may have significant effects at presynaptic terminals via activation of NMDA and metabotropic receptors (Page et al., 2005; Czaja et al., 2006).

Just as astrocytes can release gliotransmitters that alter neural circuit function, a plethora of neuronal afferent transmitters including norepinephrine, glutamate, GABA, acetylcholine, histamine, adenosine and ATP (Haydon and Carmignoto, 2006) can potently regulate calcium signaling in astrocytes. Astrocytes may also serve as metabolic sentinels (Marty et al., 2005; Haydon and Carmignoto, 2006). Astrocytes could, in turn, induce changes in autonomic regulation through release of glutamate or other gliotransmitters onto NST neurons that are central to homeostatic control. It is interesting to consider the possibility that visceral afferent inputs could influence the chemosensitivity of NST astrocytes. For example, if glia serve as important chemosensory or sentinel elements for cytokines, chemokines, hormones, nutrients, etc, then neural input to NST glial cells should certainly affect their sensitivity to these physiological parameters. One can envision an extraordinary opportunity for feedback and feedforward regulation of autonomic control reflexes through the sort of general mechanisms outlined in Figure 7. This relationship between glia and pre-and post-synaptic neural elements has been termed the “tripartite synapse” (Araque et al., 1999).

In summary, the present studies suggest that PAR1 agonists act directly on astrocytes in the NST. Activated astrocytes, in turn, excite neurons in the NST through the release of glutamate. Excitation of NST neurons that are part of gastric vago-vagal reflex control circuitry is known to be involved in normal reflex inhibition of gastric motility and the effect may be responsible for the significant reduction in gastric transit provoked by fourth ventricular thrombin or the PAR1 tethered ligand, SFLLRN-NH2. Such a mechanism may explain why bleeding, trauma [especially bleeding closed head injury], and burns causes profound gastrointestinal stasis characterized by slow transit.

Table 1.

Reagents for immunohistochemical identification

Primary antibodies Source; cat. no. dilution
PAR1 Santa Cruz; SC-8204; goat-polyclonal 1:50
NeuN Chemicon; MAB377; mouse-monoclonal 1:500
GFAP Dako; Z0334; rabbit-polyclonal 1:500
Secondary antibodies
Biotin-SP Jackson ImmunoResearch Labs; donkey anti 1:150
Rhodamine-RedX Jackson ImmunoResearch Labs; donkey anti-mouse OR 1:200
donkey anti-rabbit
Tyramide signal amplification
TSA-FITC Perkin Elmer-NEN TSA kit 1:50
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Acknowledgements

This work was supported by NIH grants HD47643, DK56373, and NS60664.

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