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. Author manuscript; available in PMC: 2020 Jun 29.
Published in final edited form as: Glia. 2020 Feb 10;68(7):1479–1494. doi: 10.1002/glia.23796

FIGURE 2.

FIGURE 2

Circulating immune cells infiltrate the MBH during PDAC. a) qRT-PCR analysis for leukocyte adhesion molecule transcripts in RNA extracted from whole hypothalamic at 10 dpi. Results are relative to sham. ***p < .001 relative to sham comparing ΔCt values in repeated measures one-way ANOVA. Results consist of two independent experiments combined. n = 4–8/group. (b) Representative 20X confocal images MBH from sham and tumor (10 dpi) mice with median eminence and arcuate nucleus labeled on sham section. ME = median eminence. ARC = arcuate nucleus. Scale bar = 100 µm. (c) Quantification of B. *p < .05 compared to sham in t-test. n = 3/group. (d) Representative 60× image of ME from BMT GFP tumor mouse, at 10 dpi. Scale bar = 20 µm. Arrows = GFP + Iba-1+ infiltrating macrophages