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. Author manuscript; available in PMC: 2022 Aug 31.
Published in final edited form as: Prostate. 2022 Feb 28;82(7):836–849. doi: 10.1002/pros.24326

Figure 2: Overview of tissue processing workflow.

Figure 2:

(A) 10% of the tissue biopsy was removed with a hand razor then sliced to 75um sections using a VF-300 Compresstome. (B) 75um tissue sections were stained with viability markers and cell-specific antibodies to evaluate tissue viability and cellular composition. (C) 90% of the tissue biopsy was used for enzymatic digestion to obtain single cell population. (D) Cell suspensions were stained with viability markers and cell-specific antibodies to evaluate cell viability and cell type. (E) Antibody-bound PMPs were added to cell suspension to bind specific cell types. (F) Extractman was used to isolate PMP-bound cell cells for flow analysis, RNA-seq, (G) cell culture, and cytotoxicity assays.