A. The location of important emerging mutations in RBD. The
Spike trimer (adapted from PDB: 7A94 (39)) viewed from the top with one RBD “up” RBD, is shown,
with individual Spike monomers colored white, gray and black. The RBM can be
topologically divided into three subsections: the “Peak” that
includes residues F486, S477, T478 and E484, the “Valley”
including residues Y453, K417 and L452 and the “Mesa” with residue
N501. Stars indicate residues on the central axis of RBD. The “Outer
Face” (exposed in the RBD down/closed conformation), and “Inner
Face” (buried inside the trimer in the RBD down/closed conformation)
define the lateral faces of RBD and “Escarpment” (contains
residues V367, N439 and glycan 343). B. NS-EM footprint of a
representative antibody from each community mapped onto an RBD monomer. The
colored shading corresponds to the community colors in Figure 1. The ACE2 binding site is outlined with a
dotted line. Side and top views of Spike trimers show the Fab approach angle and
binding stoichiometry for each representative. Table S3 shows NS-EM data for all
29 RBD-directed mAbs analyzed.