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. Author manuscript; available in PMC: 2023 Jun 5.
Published in final edited form as: Immunohorizons. 2023 Mar 1;7(3):213–227. doi: 10.4049/immunohorizons.2300008

Figure 5. An FRD may modulate some immune populations in the colonic lamina propria.

Figure 5.

Mice were fed an FRD or ND for 4 or 12 weeks before colonic lamina propria cells were isolated and analyzed by flow cytometry. tSNE analysis was performed on CD45+ cells previously gated on lymphocytes and single cells. (A) Schematic of the experimental procedure. (B) tSNE and FlowSOM analysis of flow cytometry data and (C) the number of cells in the indicated populations in each diet group. (D) Histograms from flow cytometry data depicting expression of select identifying markers by the selected populations. N=5 per group, 2-way ANOVA for bar graphs.