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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: MethodsX. 2014;1:269–274. doi: 10.1016/j.mex.2014.10.007

Fig. 2.

Fig. 2

Morphology of Iba1 immunoreactive microglia on slide mounted sections visualized after 10 mM EDTA, pH 6.0 antigen retrieval. Wild type (WT) and APP/PS1slide mounted brain sections, 10 μm, were untreated (no treatment) or processed for antigen retrieval using 10 mM EDTA, pH 6.0 before immunostaining using anti-Iba-1 antibody and visualized using vector VIP as the chromogen. Representative temporal cortex images are shown from (a) Wild type (WT) no treatment control, (b) APP/PS1 no treatment control, (c) WT 10 mM EDTA, pH 6.0 antigen retrieval, and (d) APP/PS1 10 mM EDTA, pH 6.0 antigen retrieval conditions.