Fig. 7.

Impact of 5′-Azacytidine and Hydralazine on mRNA expression levels of genes involved in CpG methylation and de-methylation in vivo. (A–D) Mice were challenged with unilateral ureteral obstruction (UUO) and treated with either vehicle buffer PBS, 5′-Azacytidine, or Hydralazine (10 mg/kg/day or 5 mg/kg/day, respectively) and sacrificed ten days after ureteral obstruction when kidneys had developed severe tubulointerstitial fibrosis. mRNA expression levels of Dnmt1 and Dnmt3a were normalized after treatment with 5′-Azacytidine and Hydralazine. Among Tet hydroxylases, Hydralazine induced expression of Tet3, but not of Tet1 or Tet2, whereas 5′-Azacytidine did not increase expression of either Tet gene. Hydralazine treatment had no effect on expression levels of cytosine deaminases Aicda, Apobec1, or Apobec3, whereas DNA glycosylases Tdg and Smug 1 were induced after Hydralazine administration in UUO mice, 5′-Azacytidine did not impact mRNA expression levels (experiments were done in triplicate, data are presented as means ± s.e.m. *p < 0.05, n.s. no significance, values of p were calculated respective to vehicle-treated UUO mice).