Body fat composition assessment using analytic morphomics predicts infectious complications following bowel resection in Crohn’s disease

Abstract

Background

Decisions between medical and surgical management of Crohn’s disease (CD) incorporate risk assessments for potential complications of each therapy. Analytic morphomics is a novel method of image analysis providing quantifiable measurements of body tissue composition, characterizing body fat more comprehensively than body mass index (BMI) alone. The aim of this study was to determine risk factors associated with post-operative complications in CD, incorporating fat composition analysis using analytic morphomics.

Methods

We performed a retrospective review of adults undergoing bowel resection for CD between 2004–2011 at a single center. CT scans within 30 days prior to surgery underwent morphomic analysis of body geometry and fat characterization. Post-operative infectious complications were defined as the need for a post-operative abdominal drain, intravenous antibiotics, or re-operation within 30 days. Bivariate and multivariate analysis using logistic regression were used to generate a prediction model of infectious complications.

Results

A total of 269 subjects met selection criteria; 27% incurred post-operative infectious complications. Bivariate analysis showed hemoglobin, albumin, surgical urgency, high-dose prednisone use, and subcutaneous-to-visceral fat volume distribution as predictors of complications. BMI, anti-TNF-alpha-therapies, and immunomodulator use were not predictors of complication. Multivariate modeling demonstrated a c-statistic of 0.77 and a NPV of 81.1% with surgical urgency (OR 2.78, 95% CI 1.46–6.02, P=.004), subcutaneous-to-visceral fat distribution (OR 2.01, 95% CI 1.20–3.19, P=.006), and hemoglobin (OR 0.69, 95% CI 0.55–0.85, P =.001) as predictors of infectious complication.

Conclusions

Fat subtype and distribution are predictive of post-operative infectious complications following bowel resection for Crohn’s disease. Analytic morphomics provides additional body composition detail not captured by BMI.

Introduction

Crohn’s disease is an immune-mediated inflammatory bowel disease with a prevalence of 136–318/100,000 in the North America¹. Modern immunomodulator and biologic therapies have improved clinical outcomes, yet nearly 50% of patients with Crohn’s disease will require surgical management within 10 years of diagnosis^2,3. In some urgent and emergent situations, such as the development of complete intestinal obstruction, large abscess or penetrating disease, surgery is unavoidable. However, in other instances the decision to pursue medical vs. surgical management is less clear, especially in the case of partially obstructing strictures which are almost invariably composed of a mixture of inflammatory and fibrotic elements⁴. Management decisions to either intensify medical therapy or proceed with surgical approaches hinge upon several data points, including objective measures of existing inflammatory disease activity. Further, risks related to surgery, including poor anastomotic and incisional wound healing following surgery are important considerations when counseling patients for surgery.

Several factors have been shown to impact the risk of complication in general abdominal surgeries including poor nutritional status and obesity⁵. Crohn’s disease has not been spared from the obesity epidemic, as several studies examining IBD have demonstrated rates of obesity approaching that of the general population⁶. While the general surgical literature has demonstrated an association between BMI and post-operative complications, the association of BMI with post-operative complications in the Crohn’s disease population has been inconsistent^{7, 8}. BMI is a course measurement that fails to capture details of body composition. Body characteristics of lean muscle mass, fat subtypes (visceral and subcutaneous), and tissue distribution can be measured using data from standard computed tomography (CT) scans⁹. Analytic morphomics provides reproducible measures of body composition, which have been associated with surgical morbidity and mortality in both liver transplant recipients and those undergoing colectomies for colon cancer^{10, 11}. Morphomic assessment offers quantitation of body composition factors related to surgical complication in Crohn’s disease, improving the counseling provided to patients regarding their individual operative risk.

In this study, we aimed to identify risk factors for infectious post-operative complication following bowel resection for Crohn’s disease, including morphometric assessments of body composition. We hypothesized that fat composition characteristics and variation of fat distribution would correlate with the incidence of infectious surgical complications.

Methods

Selection Criteria

This study was approved by the University of Michigan Institutional Review Board. Patients with Crohn’s disease undergoing surgical bowel resection between January 1, 2004 and December 31, 2011 at the University of Michigan Health System (UMHS) were identified. Crohn’s disease diagnosis was verified using one inpatient and outpatient ICD-9 code of 555.X with confirmation by review of medical records¹². Subject selection criteria included the availability of a computer tomography scan (CT) of the abdomen using standard or enterography-protocol performed within 1 month prior to abdominal surgery. Subjects undergoing surgeries where the primary indication was abscess or phlegmon were excluded considering the composite outcome of infectious post-operative complication. In addition, patients using parenteral nutrition prior to surgery were also excluded as their nutritional status could interact with the primary outcome assessment.

Clinical Data Collection

UMHS electronic medical records were reviewed using a combination of automated data extraction (University of Michigan Data Warehouse Service), natural language search engines (EMERSE), and manual chart review¹³. Patient demographics, active medications, smoking history and laboratory data immediately prior to surgery were collected. For medications, immunomodulator use was considered active if thiopurine or methotrexate use was documented within 2 weeks of surgery. Anti-tumor necrosis alpha therapy was considered active if patients were exposed to infliximab, adalimumab, or certolizumab within 8 weeks of surgery. Corticosteroid use was categorized as none, 1–20mg, 21–40mg, and >40mg. Surgical records were manually reviewed for surgical indication, type of surgery performed and American Society of Anesthesiologists (ASA) score. Surgical urgency was classified as emergent for surgery within 24 hours of admission, urgent for surgery during hospitalization, and elective for patients presenting for scheduled preoperative hospital admission.

Definition of Post-Operative Complication

The composite endpoint of post-operative infectious complication included the following criteria: (1) Use of intravenous antibiotics for an intra-abdominal infection source for at least 10 days, (2) Post-operative abdominal drain placement within 30 days of index surgery, and (3) re-operation within 30 days of index surgery. Medical records were reviewed to determine the indications of the interventions used in our composite endpoint. Infectious complications potentially related to perioperative interventions, but remote from the surgical site, including Clostridium difficile colitis, urinary tract infections, and pneumonia, were not included in the composite post-operative complication endpoint.

Analytic Morphomics

The cross-sectional areas of subcutaneous and visceral fat were measured at multiple spinal levels, from the 10^th thoracic (T10) vertebral body to the 5^th lumbar (L5) vertebral body. CT scans underwent semi-automated vertebral and soft tissue identification using image processing algorithms developed by the University of Michigan Morphomic Analysis Group in MATLAB 13.0 (MathWorks, Natick MA)¹⁴. First, individual vertebral levels were identified, serving as spatial landmarks for correlating other structures and tissues. Second-level processing included automated determination of the body perimeter defined by the transition from air (0 Hounsfield units) to skin. Definition of the fascial plane is achieved by using a nearest neighbor subtraction algorithm to delineate the zone of Hounsfield unit density change from the relatively homogenous subcutaneous fat densities to intra-abdominal non-fat tissues with higher densities. Following these regional definitions, subcutaneous and visceral fat were quantified by area at each spinal level (Figure 1). To control for variations in body size, subcutaneous and visceral fat measurements were standardized to total body cross sectional area at each spinal level. Fat volumes were calculated by multiplying fat area by vertebral body height at each spinal level. To assess the impact of variation in fat distribution by body region, we assessed the relative degree of subcutaneous and visceral fat (standardized to total body volume) over several regions. Total fat volumes within a spinal range were calculated by summation of individual level volumes (e.g. T10:L5). The relative change in fat distribution across spinal levels was defined as the ratio of subcutaneous to visceral fat measurements between the most cephalad and caudal spinal levels within the abdominal field (e.g. subcutaneous fat to visceral fat ratio at T10 divided by the subcutaneous fat to visceral fat ratio at L5). In addition to fat characteristics, other morphomic body parameters, including body geometries of anterior-posterior distances and body eccentricity, were measured. All morphomic measurements underwent quality control assessment. A single reviewer (GS) reviewed the results of automated spinal level, fascial plane, and fat area identification, as occasionally imaging artifact led to inaccurate automated tissue identification.

Figure 1. Subcutaneous and visceral fat definitions by automated morphometric analysis methodology.

Computed tomography scans are processed by image boundary definition routines written in Matlab. Vertebral body definitions are verified by a human operator and corrected when necessary (a). Fascial plane identification (b, yellow line) serves as boundary between subcutaneous (b) and visceral fat (c) at midpoints of spinal levels. Fat volumes are the product of fat area and vertebral body height as individual spinal levels.

Statistical Analysis

Demographic, clinical, and laboratory features were compared between patients with and without complications using the Fisher’s exact test and the Mann–Whitney Wilcoxon rank-sum test for categorical and continuous variables, respectively. Simple linear regression was used to compare BMI to morphomic fat measurements. Bivariate analysis by logistic regression was performed using the composite post-operative complication endpoint as the dependent variable. Regression models were developed using variables identified with a P < .05 on bivariate analysis, as well as those determined a priori to be clinical relevant. Multicollinearity was assessed by reviewing the correlation matrix and variance inflation factor analysis of covariates (VIF) included in the model. The discriminatory power of the regression models were evaluated using receiver operating characteristic (ROC) curve analysis. C-statistics in ROC analysis range from 0.5 to 1.0, with values of 0.5 indicating the model is no better than chance and 1.0 indicating the model perfectly discriminates the two groups. Models are considered reasonable if the c-statistic exceeds 0.70 and strong if greater than 0.80. All analyses were conducted using STATA statistical software 13.0 (College Station, TX) and R statistical package 2.14.0.

Results

Cohort characteristics

Within the 7-year period reviewed, 364 patients with Crohn’s disease underwent surgical bowel resection for obstructive disease or medication non-responsiveness at the University of Michigan Health System. Ninety-five (95) patients were excluded because of unsuitable CT scans, typically due to abbreviated CT scans terminating at L2–L3 spinal levels or the use of imaging formats unable to be converted to the DICOM standard. A total of 269 patients were analyzed. Subjects were grouped by the occurrence of an infectious surgical complication, with an observed complication rate of 27%. Patient mean age was 35.9±15.8 years, 45.9% were male and the cohort was overwhelmingly Caucasian (89.6%). When BMI was categorized as underweight (≤18.5 kg/m²), normal (>18.5–24.5 kg/m²), overweight (>24.5–30 kg/m²), or obese (≥30 kg/m²), there was no significant difference in complications among BMI categories using Fisher’s exact test (P = .193).

When considering medication usage across all patients, mean immunomodulator use was 37.5%, mean anti-TNF use within 8 weeks of surgery was 33.1%, and use of any corticosteroid was 44.9%. A corticosteroid dose of 40mg or more was more frequently observed in those with surgical complications (7.7% vs. 13.7%, P = .041), however doses less than 40mg were not associated with complications in this study. Outside of high-dose prednisone exposure, no other patient characteristics significantly differed between those with and without surgical complications, including gender, age, ethnicity, smoking status, diabetes mellitus, immunomodulator and anti-TNF use, and American Society of Anesthesiologists (ASA) score (Table 1).

Table 1.

Patient characteristics at the time of surgery

Characteristic		No Complication (n=196)	Complication (n=73)	P value
Male (%)		90 (45.9)	33 (45.2)	0.51
Age		35.9±15.8	35.8 (17.3)	0.919
Ethnicity (%)				0.823
Caucasian		175 (89.3)	66 (90.4)
African Amer.		18 (9.1)	6 (8.2)
Other		2 (1.0)	1 (1.4)
BMI Category				0.193
Underweight	(BMI <18.5)	43 (21.9)	14 (19.2)
Normal	(BMI 18.5–25)	87 (44.4)	43 (58.9)
Overweight	(BMI 25–30)	46 (26.5)	11 (15.1)
Obese	(BMI >30)	20 (10.2)	5 (6.8)
Medication Use (%)
Prednisone		83(42.3)	38 (52.0)	0.361
	<20mg	30 (15.3)	11 (15.1)	0.432
	20–40mg	38 (19.4)	17 (23.2)	0.124
	>40mg	15 (7.7)	10 (13.7)	0.041
Immunomodulator		75 (38.3)	26 (35.6)	0.249
Anti-TNF Use		66 (33.6)	23 (31.5)	0.284
Smoking, Active (%)		45 (23.0)	13 (17.8)	0.224
Diabetes mellitus (%)		15 (7.6)	6 (8.2)	0.899
ASA Score, mean		2.40±0.5	2.45±0.5	0.557
Laboratory Values
White blood cell count (k3/mL)		9.85 (±5.42)	9.67 (±5.86)	0.835
Hemoglobin (g/dL)		11.96 (±1.91)	11.13 (±2.21)	<0.001
Platelets (k2/mL)		279.5 (±159.6)	294.19 (±141.4)	0.140
Albumin (g/dL)		3.60 (±0.70)	3.30 (±0.78)	0.007

Complete blood counts and comprehensive metabolic panels were collected within 48 hours prior to surgery for all patients. On bivariate analysis, lower hemoglobin values were a predictor of surgical complication (11.96 g/dL vs. 11.13g/dL, P < .001). In addition, lower serum albumin levels were observed in those with surgical complications (3.60 g/dL vs. 3.30 g/dL, P = .007). White blood cell count, platelet count, blood urea nitrogen, and serum creatinine measurements did not differ significantly between those with and without surgical complications. C-reactive protein (CRP) was not included in the analysis, as values were only available for 36% of subjects.

Surgical characteristics

Ileocecectomy was the most common surgery performed (62.5%), followed by hemicolectomy (20.0%). The type of surgery performed was not associated with an increased rate of complications. Unsurprisingly, patients undergoing a primary anastomosis without diversion had a statistically significant increased probability of infectious complications (68.4% vs. 52.1%, P = .022). Surgical urgency and the timing of surgery were associated with an increased risk of surgical complication (Table 2). Those undergoing emergent (within 24 hours of admission) and urgent surgery (during hospitalization) developed surgical complications more frequently than those undergoing elective surgery (36.7% vs. 18.4%, P, < .001). Of those where surgical complication occurred (n = 73, 27%) 71% received IV antibiotics for an intra-abdominal or wound infection, 41% required a post-operative drain placement, and 32% underwent repeat surgery within 30 days. Examination of the surgeries performed on a weekend date (Saturday, Sunday) did not significantly increase risk of surgical complication on bivariate analysis.

Table 2.

Operative characteristics in patients undergoing bowel resection for Crohn's disease

			Bivariate Analysis
	No Complication (n=196)	Complication (n=73)	Odds Ratio	95% CI	p value
Surgical Urgency (%)			2.80	1.73–4.51	<0.001
Elective	115 (58.7)	26 (35.6)	1.00	Reference
Urgent	74 (37.8)	38 (52.1)	2.72	1.45–5.10	0.002
Emergent	7 (3.8)	9 (12.3)	8.12	2.70–24.39	<0.001
Weekend Surgery	35 (17.9)	14 (19.1)	1.43	0.71–2.88	0.312
Surgical Type (%)					0.215
Ileocecectomy	124 (62.2)	46 (63.0)	1.00	Reference
Hemicolectomy	34 (17.3)	20 (27.4)	1.38	0.71–2.72	0.345
Small Bowel Resection	38 (19.4)	7 (9.6)	0.44	0.13–1.54	0.198
Anastomosis Without Diversion	134 (68.4)	38 (52.1)	1.95	1.10–3.49	0.022

Body mass, geometry, and fat distribution analysis

Traditional measurements of body mass were not associated with the development of surgical complications. BMI measurement within 30 days of CT imaging for those without and with surgical complication was 22.5±7.9 kg/m² vs. 21.9±6.0 kg/m², P = .919 (Table 3).. Standardized subcutaneous fat volume ratios (volume of fat standardized to body volume) between T10 and L5 spinal levels did not demonstrate significant differences between those with and without complications (0.38 vs. 0.40, P = .54). Further, standardized visceral fat volume between T10:L5 showed no significant associate with surgical complication rate. However, the relative proportion of fat subtype between the T10 and L5 was significant. Subcutaneous to visceral fat ratios were significantly greater the in caudal region in those with post-operative complication (0.95 vs. 0.62, P = .012). The subcutaneous-to-visceral fat ratio was very weakly correlated with BMI (R² = 0.0239, P = .007). BMI was not correlated with standardized subcutaneous fat measurements, (P = .869, R² = 0.0001). To determine if the distribution of fat subtype was merely a surrogate of body geometry, central adiposity shape information was assessed using anterior to posterior distance and body eccentricity; neither were predictors of complication status.

Table 3.

Morphometric measurements of body fat distribution

	No Complication (n=196)	Complication (n=73)	Bivariate Analysis
	Mean (SD)	Mean (SD)	Odds Ratio	95% CI	p value
BMI, kg/m2	22.5 (±7.9)	21.9 (±6.0)	0.99	0.96–1.04	0.919
Total Ant-Post Distance – T10, mm	237.4 (±38.5)	230.7 (±38.1)	0.99	0.99–1.00	0.334
Body Eccentricity – T10	0.68 (±0.06)	0.68 (±0.06)	2.8	0.02–443.70	0.691
Percent Visceral Fat Volume – T10:L5, %	0.67 (±0.67)	0.56 (±0.36)	0.58	0.26–1.29	0.179
Percent Subcutaneous Fat Volume – T10:L5,%	0.38 (±0.20)	0.40 (±0.27)	1.49	0.42–5.31	0.537
Subcutaneous-to-Visceral Fat Ratio – T10:L5	0.62 (±0.41)	0.95 (±0.69)	1.73	1.13–2.66	0.012

Multivariate model of post-operative complication

A multivariate model of risk for post-operative infectious complications following bowel resection was constructed using subcutaneous-to-visceral fat ratio between T10:L5, hemoglobin, albumin, recent exposure to prednisone >40mg/day, and surgical urgency, with additional adjustment for gender, age, diabetes, smoking, and ASA scores (Table 4). Emergent or urgent surgical bowel resection increased the odds of complication (OR 2.78 95% CI 1.48–6.02, P = .004). Patients with a higher ratio of subcutaneous to visceral fat in the central region (T10) relative to the mid-lower abdominal region (L5) were more likely to experience a complication in the multivariate model (OR 2.01, 95% CI 1.20–3.19, P = .006). Albumin and prednisone dosage were not significant contributors to the risk of surgical complications after adjusting for covariates in this multivariate model. Multicollinearity of covariates was not detected and VIF values were less than 3.0. Receiver operating curve of model performance yielded a c-statistic of 0.77, NPV of surgical complication of 81.1% (Figure 2).

Table 4.

Multivariate model for surgical complications in Crohn's disease

	Odds Ratio	95% CI	p value
Surgical Urgency	2.78	1.48–6.02	0.004
Subcutaneous-to-Visceral Fat Ratio – T10:L5	2.01	1.20–3.19	0.006
Hemoglobin	0.69	0.55–0.85	0.001
Albumin	1.30	0.72–2.35	0.372
Prednisone >40mg/day	1.05	0.76–1.44	0.792
Gender	1.50	0.71–3.17	0.283
Age	1.00	0.78–3.14	0.926
Diabetes	0.67	0.11–3.73	0.618
Active Smoking	0.69	0.29–1.64	0.406
ASA Score	0.86	0.45–1.63	0.635

Figure 2. Receiver operating curve for the multivariate model predicting post-operative complications.

Multivariate model for post-operative surgical complications following Crohn’s disease-related bowel resection demonstrated an OR 1.83, 95% CI 1.19–2.34 (P = .021). Reduced hemoglobin, surgical urgency, and elevated subcutaneous fat ratio between T10:L5 were the most predictive variables in the model. The c-statistic of this model was 0.77 with a NPV of 81.1% for post-operative complication.

Discussion

In this study, we show that an increased degree of subcutaneous relative to visceral fat increases the risk of infectious complication following bowel resection in Crohn’s disease. Despite this finding, BMI was not predicative of surgical complication in this single-center cohort. The discrepancy between BMI and subcutaneous-visceral fat measurements ability to predict complications suggests that careful measurement of both fat subtype and distribution provides more useful data than the relatively coarse measure of BMI. Further, there was very little correlation between BMI and the morphomic measurements of standardized fat volumes. In addition to providing a model for post-operative complications in Crohn’s disease, this study demonstrates the utility of quantitative measures more representative of tissue composition and distribution in inflammatory bowel disease.

Our multivariate model for predicting post-surgical infectious complication includes several individual parameters previously shown to increase risk of surgical complications in Crohn’s disease. Surgical urgency has been reported to increase the rate of surgical complications, likely the result of more severe disease phenotypes combined with the potential for limited availability of colorectal expertise in emergent presentations¹⁵. It is important to emphasize that patients with a known abscess or perforation were excluded from this study, as preoperative peritoneal contamination could confound outcome assessment. Considering the laboratory parameters studied, low hemoglobin levels were a strong component in the model, potentially reflecting the chronicity of poorly controlled disease¹⁶. Serum albumin levels less than 3.5 g/dL have been previously reported to predict post-operative morbidity in both general abdominal surgery and in IBD-related surgery^17–19. However, albumin was significant in the adjusted multivariate model, potentially because disease chronicity and subsequent malnutrition impacting wound healing and infection risk are better captured by hemoglobin or fat distribution. Corticosteroids at doses greater than 40mg are known to increase post-operative infectious complications in IBD patients^{20, 21}. While high dose corticosteroids were associated with post-operative complications on bivariate analysis, high dose prednisone was not significant in this multivariate model. Because intermediate and long term corticosteroids are likely to impact body fat, the relatively low contribution of steroids in this risk model may be due to collinearity of fat changes and steroid use.

Increased proportion of subcutaneous relative to visceral fat volume distributed more in the thoracic vs. lumbar spinal regions was a significant predictor of post-operative complications in our multivariate model. Khoury et al. found that obese patients (defined as BMI >30kg/m²) undergoing laparoscopic bowel resection (not limited to IBD) had significantly higher rates of wound infection, conversion to open procedures, and lengthened hospital stay relative to non-obese patients⁵. Studies assessing the impact of obesity on surgical outcomes in Crohn’s disease have had variable results. A retrospective study of outcomes following Crohn’s disease related surgery (n = 2319) found that obesity assessed by BMI conferred an increased risk of wound and intrabdominal infection⁷. However, outside of the Causey et al. study, both our data and several other groups have not found an association between traditional measures of body mass including weight, BMI, and body geometry with surgical complications in IBD^{16, 22, 23}. BMI is unable to capture changes in fat distribution and overall body composition, including variations in subcutaneous and visceral fat. Variations in fat volume and distribution may impact surgical outcomes simply by making tissue approximation more difficult. Morphomics allows careful quantitation of body composition changes typically assessed by subjective physician assessments.

Outside of the role of fat composition and distribution, adiposity itself may affect disease activity and outcomes in IBD. Patients with IBD are not immune from the obesity epidemic. In North America and Europe, BMI values in CD patients have steadily increased from 20.8 in 1991 to 27.0 in 2001, mirroring weight gain trends in the general population^{6, 24–26}. Further, increased adiposity has been associated with systemic inflammation, increased intestinal permeability, and alterations in the intestinal microbiome^27–29. These findings suggest that adiposity may have a role in the development, phenotype, and severity of inflammatory bowel disease. A few retrospective studies have demonstrated an elevated BMI (>25kg/m²) was associated with shorter time from IBD diagnosis to first surgery and a higher incidence of perianal penetrating disease^{30, 31}.

BMI is a simple, zero-cost tool to measure mass distribution based on height, however BMI fails to characterize fat composition. Despite the biologic plausibility and connections between obesity and inflammation, BMI itself has not been directly associated with clinical disease activity scores, disease phenotype, or incidence of IBD³². Studies in both adult and pediatric populations have demonstrated that BMI is a poor indicator of nutritional status, body fat, and lean mass content when compared to anthropometric measures of triceps skin fold thickness and bioelectrical impedance analysis^{33, 34}. A systematic review reported that over a third of patients with Crohn’s disease had altered body composition compared to control³⁵. Other objective measures of body fat composition including hydrodensitrometry and bioelectrical impedance testing provide reproducible results, but can be time consuming and costly. Morphomic assessment may highlight the body composition changes occurring in IBD that are not apparent when assessing patients using BMI³⁶. Considering the frequency of imaging in subjects with IBD, analytic morphomic analysis provides a quantitative, high throughput, value-added measurement of body composition which may aid investigations examining the impact of obesity in IBD.

Several limitations must be considered when interpreting the results of our work. First, our composite outcome of post-operative infectious complication was designed to have high specificity over sensitivity, which may result in failing to capture all post-operative events. Second, we were unable to accurately differentiate isolated wound infection versus anastomotic leak or direct bowel-related infectious complication. The risk factors identified may further segregate between these two regions of infection. Third, decisions on surgical timing, pre-operative optimization strategies and surgical methods were not standardized among providers, introducing selection bias into the results. Surgical approach and techniques were likely to have varied based on patient condition; this cannot be controlled in the context of a retrospective cohort study design. Further, we did not stratify our subjects based on underlying disease severity or activity, as insufficient information was available to meaningfully characterize cumulative disease activity. Finally, the impact of anti-TNF therapies on post-operative infection remains debated³⁷. Insufficient data was available to determine proximity of anti-TNF administration to surgery in our cohort; time to exposure may prove to be an important variable in anti-TNF associated surgical risk.

This multivariate model of post-operative infectious complication in Crohn’s disease may help physicians better individualize operative risk when discussing surgery with patients. Certainly several situations, regardless of risk, demand surgical management including bowel obstruction, large abscesses, or penetrating disease.. While modification of the factors in this model are unlikely to immediately reduce their risk of operative complication, such models can serve as a clinical guide to better quantify the factors contributing to individual surgical risk.

As we continue to examine the interplay between intestinal inflammation and obesity, tools providing more granular measures of body composition, like analytic morphomics, will be important for studying the role of adiposity on disease outcomes. While subject to the limitations of a retrospective study design, this data indicates that more detailed fat assessment may aid in the prediction of post-operative infectious complications in Crohn’s disease. Quantitative body composition assessments using analytic morphomic techniques may provides a new quantitative tool for studying the impact of adiposity on Crohn’s and other inflammatory diseases.