Neonatal treatment with NBI 35965 prevented vaginal and hindpaw hypersensitivity in NVI mice. A) The VMR during VBD was significantly lower in NBI 35965-treated NVI mice (n=11) compared to saline-treated NVI mice (n=8) over the entire distension period (p < 0.05), as well as at the two highest pressures applied. Naïve mice treated with saline (n=8) or NBI 35965 (n=8) displayed similar VMR during VBD. B) The VMR during CRD did not differ between any of the groups (n=7–9/group). Neonatal treatment with NBI 35965 significantly attenuated the decrease in mechanical withdrawal threshold (C) and thermal withdrawal latency (D) in NVI mice (n=13) compared to saline-treated NVI mice (n=11). Naïve mice treated with saline (n=10) or NBI 35965 (n=8) had similar mechanical withdrawal thresholds (C) and thermal withdrawal latencies (D). A–B: #,##p < 0.05, 0.01 vs. saline-treated; two-way RM ANOVA and Bonferroni posttest; C–D: ***,****p < 0.001, 0.0001 vs. naïve, ##,####p < 0.01, 0.0001 vs. saline-treated; two-way ANOVA and Bonferroni posttest.