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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jul 3;484:313–322. doi: 10.1016/j.virol.2015.06.016

Fig. 3.

Fig. 3

Impact of SUD and SUD-NM proteins, provided in trans, on the activity of the full-length and SUD-deleted SARS-CoV replicons. Indicated DNAs were co-transfected in Huh-T7 cells (A) or additionally infected with vaccinia virus MVA-T7 as a helper virus (see Materials and Methods) (B). The infection and/or co-transfection mixtures were replaced with growth medium and incubated for an additional 48 hrs. The cell lysates were analyzed for Renilla luciferase (A and B, p < 0.01) and anti-His immunological activity in mixtures 2 and 3 shown in panel B (C). All experiments were run in triplicate and the average was employed for analysis. Error bars represent standard deviations of the mean values. The difference in reporter protein activity of the full-length replicon and the SUD-lacking replicon was found to be statistically significant (panel A, p = 0.005; panel B, p = 0.01).