Abstract
The effect of motilin on lower oesophageal sphincter (LES) pressure has been studied in unanesthetised specially trained dogs using an infusion manometric technique. Motilin produced significant rises in resting pressure and contractions of the LES after doses ranging from 0-009 mug/kg to 0-05 mug/kg. Doses greater than 0-05 mug/kg resulted in repetitive high amplitude contractions. Atropine 30 mug/kg completely abolished the effect of the lower doses of motilin. Higher doses of motilin in atropinised dogs still caused a small rise in baseline pressure and contractile activity still appeared. Hexamethonium 2 mg/kg resulted in both a diminished rise in LES pressure and the disappearance of contractions after motilin. Hexamethonium and atropine together completely abolished the LES response to motilin. We conclude that motilin increases LES pressure by acting on preganglionic cholinergic neurones to release acetylcholine which excites other cholinergic neurones supplying the circular muscle of the LES.
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Selected References
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- Brown J. C., Cook M. A., Dryburgh J. R. Motilin, a gastric motor activity stimulating polypeptide: the complete amino acid sequence. Can J Biochem. 1973 May;51(5):533–537. doi: 10.1139/o73-066. [DOI] [PubMed] [Google Scholar]
- Brown J. C., Cook M. A., Dryburgh J. R. Motilin, a gastric motor activity-stimulating polypeptide: final purification, amino acid composition, and C-terminal residues. Gastroenterology. 1972 Mar;62(3):401–404. [PubMed] [Google Scholar]
- Brown J. C., Johnson L. P., Magee D. F. Effect of duodenal alkalinization of gastric motility. Gastroenterology. 1966 Mar;50(3):333–339. [PubMed] [Google Scholar]
- Brown J. C., Mutt V., Dryburgh J. R. The further purification of motilin, a gastric motor activity stimulating polypeptide from the mucosa of the small intestine of hogs. Can J Physiol Pharmacol. 1971 May;49(5):399–405. doi: 10.1139/y71-047. [DOI] [PubMed] [Google Scholar]
- Cohen S., Lipshutz W. Hormonal regulation of human lower esophageal sphincter competence: interaction of gastrin and secretin. J Clin Invest. 1971 Feb;50(2):449–454. doi: 10.1172/JCI106512. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diamant N. E., Akin A. N. Effect of gastric contractions on the lower esophageal sphincter. Gastroenterology. 1972 Jul;63(1):38–44. [PubMed] [Google Scholar]
- Goyal R. K., Rattan S. Nature of the vagal inhibitory innervation to the lower esophageal sphincter. J Clin Invest. 1975 May;55(5):1119–1126. doi: 10.1172/JCI108013. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jennewein H. M., Hummelt H., Siewert R., Waldeck F. The motor-stimulating effect of natural motilin on the lower esophageal sphincter fundus, antrum and duodenum in dogs. Digestion. 1975;13(4):246–250. doi: 10.1159/000197714. [DOI] [PubMed] [Google Scholar]
- Nebel O. T., Castell D. O. Lower esophageal sphincter pressure changes after food ingestion. Gastroenterology. 1972 Nov;63(5):778–783. [PubMed] [Google Scholar]
- Sarna S. K., Daniel E. E. Threshold curves and refractoriness properties of gastric relaxation oscillators. Am J Physiol. 1974 Apr;226(4):749–755. doi: 10.1152/ajplegacy.1974.226.4.749. [DOI] [PubMed] [Google Scholar]
- Strunz U., Domschke W., Mitznegg P., Domschke S., Schubert E., Wünsch E., Jaeger E., Demling L. Analysis of the motor effects of 13-norleucine motilin on the rabbit, guinea pig, rat, and human alimentary tract in vitro. Gastroenterology. 1975 Jun;68(6):1485–1491. [PubMed] [Google Scholar]
- Thomas P. A., Earlam R. J. The action of gastro-intestinal polypeptide hormones on the isolated perfused gastro-oesophageal sphincter. Br J Surg. 1973 Apr;60(4):306–306. [PubMed] [Google Scholar]
- Zwick R., Bowes K. L., Daniel E. E., Sarna S. K. Mechanism of action of pentagastrin on the lower esophageal sphincter. J Clin Invest. 1976 Jun;57(6):1644–1651. doi: 10.1172/JCI108435. [DOI] [PMC free article] [PubMed] [Google Scholar]