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. 2001 Aug;10(4):173–178. doi: 10.1080/09629350124119

Effects after inhalation of (1-->3)-beta-D-glucan in healthy humans.

J Thorn 1, L Beijer 1, R Rylander 1
PMCID: PMC1781709  PMID: 11577993

Abstract

BACKGROUND AND AIM: This study was performed to assess the effects of an exposure to a pure (1-->3)-beta-D-glucan, a cell wall component of fungi, plants and certain bacteria. METHODS: Twenty-one healthy subjects inhaled saline or (1-->3)-beta-D-glucan suspended in saline in a random, double-blind, cross-over design. They were examined before exposure and 24 and 72h afterwards with spirometry, blood sampling and collection of induced sputum. Differential cell counts and eosinophilic cationic protein (ECP) were determined in blood and sputum, and myeloperoxidase (MPO), tumour necrosis factor-alpha (TNF-alpha), and interleukin (IL)-8 and IL-10 were determined in sputum supernatants. TNF-alpha was determined after cultivation of blood mononuclear cells. RESULTS: In sputum, inhalation of saline caused a significant increase in ECP and TNF-alpha. (1-->3)-beta-D-Glucan inhalation caused a further increase in these cytokines, although not statistically significantly different from the increase induced by inhalation of saline alone. In blood, the number of eosinophils was significantly decreased 72 h after the challenge with (1-->3)-beta-D-glucan. This effect was not found after the inhalation of saline alone. TNF-alpha production from stimulated blood mononuclear cells was significantly decreased 72 h after the (1-->3)-beta-D-glucan inhalation as compared with the increase induced by saline inhalation. CONCLUSIONS: The results suggest that (1-->3)-beta-D-glucan causes a different type of response as compared with inflammatory agents such as bacterial endotoxin that cause a neutrophil-dominated inflammatory response.

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Selected References

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  1. Adachi Y., Okazaki M., Ohno N., Yadomae T. Enhancement of cytokine production by macrophages stimulated with (1-->3)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa. Biol Pharm Bull. 1994 Dec;17(12):1554–1560. doi: 10.1248/bpb.17.1554. [DOI] [PubMed] [Google Scholar]
  2. Castagnaro A., Chetta A., Foresi A., D'Ippolito R., Malorgio R., Olivieri D. Effect of sputum induction on spirometric measurements and arterial oxygen saturation in asthmatic patients, smokers, and healthy subjects. Chest. 1999 Oct;116(4):941–945. doi: 10.1378/chest.116.4.941. [DOI] [PubMed] [Google Scholar]
  3. Cerning J. Exocellular polysaccharides produced by lactic acid bacteria. FEMS Microbiol Rev. 1990 Sep;7(1-2):113–130. doi: 10.1111/j.1574-6968.1990.tb04883.x. [DOI] [PubMed] [Google Scholar]
  4. Cook J. A., Dougherty W. J., Holt T. M. Enhanced sensitivity to endotoxin induced by the RE stimulant, glucan. Circ Shock. 1980;7(3):225–238. [PubMed] [Google Scholar]
  5. Di Luzio N. R. Update on the immunomodulating activities of glucans. Springer Semin Immunopathol. 1985;8(4):387–400. doi: 10.1007/BF01857392. [DOI] [PubMed] [Google Scholar]
  6. Estrada A., Yun C. H., Van Kessel A., Li B., Hauta S., Laarveld B. Immunomodulatory activities of oat beta-glucan in vitro and in vivo. Microbiol Immunol. 1997;41(12):991–998. doi: 10.1111/j.1348-0421.1997.tb01959.x. [DOI] [PubMed] [Google Scholar]
  7. Fogelmark B., Goto H., Yuasa K., Marchat B., Rylander R. Acute pulmonary toxicity of inhaled beta-1,3-glucan and endotoxin. Agents Actions. 1992 Jan;35(1-2):50–56. doi: 10.1007/BF01990951. [DOI] [PubMed] [Google Scholar]
  8. Fogelmark B., Sjöstrand M., Rylander R. Pulmonary inflammation induced by repeated inhalations of beta(1,3)-D-glucan and endotoxin. Int J Exp Pathol. 1994 Apr;75(2):85–90. [PMC free article] [PubMed] [Google Scholar]
  9. Fogelmark B., Thorn J., Rylander R. Inhalation of (1-->3)-beta-D-glucan causes airway eosinophilia. Mediators Inflamm. 2001 Feb;10(1):13–19. doi: 10.1080/09629350123707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knudson R. J., Lebowitz M. D., Holberg C. J., Burrows B. Changes in the normal maximal expiratory flow-volume curve with growth and aging. Am Rev Respir Dis. 1983 Jun;127(6):725–734. doi: 10.1164/arrd.1983.127.6.725. [DOI] [PubMed] [Google Scholar]
  11. Matricardi P. M., Nisini R., Pizzolo J. G., D'Amelio R. The use of Phadiatop in mass-screening programmes of inhalant allergies: advantages and limitations. Clin Exp Allergy. 1990 Mar;20(2):151–155. doi: 10.1111/j.1365-2222.1990.tb02660.x. [DOI] [PubMed] [Google Scholar]
  12. Michel O., Ginanni R., Le Bon B., Content J., Duchateau J., Sergysels R. Inflammatory response to acute inhalation of endotoxin in asthmatic patients. Am Rev Respir Dis. 1992 Aug;146(2):352–357. doi: 10.1164/ajrccm/146.2.352. [DOI] [PubMed] [Google Scholar]
  13. Michel O., Nagy A. M., Schroeven M., Duchateau J., Nève J., Fondu P., Sergysels R. Dose-response relationship to inhaled endotoxin in normal subjects. Am J Respir Crit Care Med. 1997 Oct;156(4 Pt 1):1157–1164. doi: 10.1164/ajrccm.156.4.97-02002. [DOI] [PubMed] [Google Scholar]
  14. Pin I., Gibson P. G., Kolendowicz R., Girgis-Gabardo A., Denburg J. A., Hargreave F. E., Dolovich J. Use of induced sputum cell counts to investigate airway inflammation in asthma. Thorax. 1992 Jan;47(1):25–29. doi: 10.1136/thx.47.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pretus H. A., Ensley H. E., McNamee R. B., Jones E. L., Browder I. W., Williams D. L. Isolation, physicochemical characterization and preclinical efficacy evaluation of soluble scleroglucan. J Pharmacol Exp Ther. 1991 Apr;257(1):500–510. [PubMed] [Google Scholar]
  16. Rylander R., Thorn J., Attefors R. Airways inflammation among workers in a paper industry. Eur Respir J. 1999 May;13(5):1151–1157. doi: 10.1034/j.1399-3003.1999.13e35.x. [DOI] [PubMed] [Google Scholar]
  17. Sakurai T., Ohno N., Yadomae T. Changes in immune mediators in mouse lung produced by administration of soluble (1-->3)-beta-D-glucan. Biol Pharm Bull. 1994 May;17(5):617–622. doi: 10.1248/bpb.17.617. [DOI] [PubMed] [Google Scholar]
  18. Schwartz D. A., Thorne P. S., Yagla S. J., Burmeister L. F., Olenchock S. A., Watt J. L., Quinn T. J. The role of endotoxin in grain dust-induced lung disease. Am J Respir Crit Care Med. 1995 Aug;152(2):603–608. doi: 10.1164/ajrccm.152.2.7633714. [DOI] [PubMed] [Google Scholar]
  19. Sherwood E. R., Williams D. L., McNamee R. B., Jones E. L., Browder I. W., Di Luzio N. R. Enhancement of interleukin-1 and interleukin-2 production by soluble glucan. Int J Immunopharmacol. 1987;9(3):261–267. doi: 10.1016/0192-0561(87)90049-x. [DOI] [PubMed] [Google Scholar]
  20. Sjöstrand M., Rylander R. Pulmonary cell infiltration after chronic exposure to (1-->3)-beta-D-glucan and cigarette smoke. Inflamm Res. 1997 Mar;46(3):93–97. doi: 10.1007/s000110050108. [DOI] [PubMed] [Google Scholar]
  21. Soltys J., Quinn M. T. Modulation of endotoxin- and enterotoxin-induced cytokine release by in vivo treatment with beta-(1,6)-branched beta-(1,3)-glucan. Infect Immun. 1999 Jan;67(1):244–252. doi: 10.1128/iai.67.1.244-252.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thorn J., Rylander R. Inflammatory response after inhalation of bacterial endotoxin assessed by the induced sputum technique. Thorax. 1998 Dec;53(12):1047–1052. doi: 10.1136/thx.53.12.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Torén K., Brisman J., Järvholm B. Asthma and asthma-like symptoms in adults assessed by questionnaires. A literature review. Chest. 1993 Aug;104(2):600–608. doi: 10.1378/chest.104.2.600. [DOI] [PubMed] [Google Scholar]
  24. Williams D. L., Pretus H. A., McNamee R. B., Jones E. L., Ensley H. E., Browder I. W., Di Luzio N. R. Development, physicochemical characterization and preclinical efficacy evaluation of a water soluble glucan sulfate derived from Saccharomyces cerevisiae. Immunopharmacology. 1991 Nov-Dec;22(3):139–155. doi: 10.1016/0162-3109(91)90039-2. [DOI] [PubMed] [Google Scholar]
  25. in 't Veen J. C., de Gouw H. W., Smits H. H., Sont J. K., Hiemstra P. S., Sterk P. J., Bel E. H. Repeatability of cellular and soluble markers of inflammation in induced sputum from patients with asthma. Eur Respir J. 1996 Dec;9(12):2441–2447. doi: 10.1183/09031936.96.09122441. [DOI] [PubMed] [Google Scholar]

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