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. 1992 Dec;66(6):1059–1064. doi: 10.1038/bjc.1992.410

Short-term dietary copper deficiency does not inhibit angiogenesis in tumours implanted in striated muscle.

D A Schuschke 1, M W Reed 1, J T Saari 1, M D Olson 1, D M Ackermann 1, F N Miller 1
PMCID: PMC1978058  PMID: 1280989

Abstract

The effect of dietary copper deficiency on tumour growth, neovascularisation and microvascular integrity was studied in the rat cremaster muscle. Male, weanling Sprague-Dawley rats were fed purified diets which were copper deficient (< 0.5 micrograms g-1 of diet) or copper adequate (5 micrograms g-1 of diet). Seven days after initiation of diets, a chondrosarcoma was implanted in the cremaster muscle of each rat. Five, 10 or 20 days after tumour implantation, rats were anesthetised and their cremasters prepared for observation by intravital microscopy. Intraarterial injection of fluorescein isothiocyanate-conjugated albumin and subsequent observation of fluorescence in the perivascular space indicated no difference in microvascular albumin leakage between the tumour vasculature of copper deficient and copper adequate rats. Neither tumour growth (assessed by wet weight), vascular density (assessed by light microscopy), nor any ultrastructural characteristics of the tumour or its vasculature (assessed by electron microscopy) were affected by copper deficiency. In view of findings by others which indicate changes in tumour characteristics with copper deficiency, we conclude that the copper dependency of tumour growth and vascularisation is a function of the type of tumour, the host tissue, or the conditions of copper depletion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brem S. S., Zagzag D., Tsanaclis A. M., Gately S., Elkouby M. P., Brien S. E. Inhibition of angiogenesis and tumor growth in the brain. Suppression of endothelial cell turnover by penicillamine and the depletion of copper, an angiogenic cofactor. Am J Pathol. 1990 Nov;137(5):1121–1142. [PMC free article] [PubMed] [Google Scholar]
  2. Brem S., Cotran R., Folkman J. Tumor angiogenesis: a quantitative method for histologic grading. J Natl Cancer Inst. 1972 Feb;48(2):347–356. [PubMed] [Google Scholar]
  3. Brem S., Tsanaclis A. M., Zagzag D. Anticopper treatment inhibits pseudopodial protrusion and the invasive spread of 9L gliosarcoma cells in the rat brain. Neurosurgery. 1990 Mar;26(3):391–396. doi: 10.1097/00006123-199003000-00003. [DOI] [PubMed] [Google Scholar]
  4. Farquharson C., Duncan A., Robins S. P. The effects of copper deficiency on the pyridinium crosslinks of mature collagen in the rat skeleton and cardiovascular system. Proc Soc Exp Biol Med. 1989 Nov;192(2):166–171. doi: 10.3181/00379727-192-42973. [DOI] [PubMed] [Google Scholar]
  5. Folkman J., Klagsbrun M. Angiogenic factors. Science. 1987 Jan 23;235(4787):442–447. doi: 10.1126/science.2432664. [DOI] [PubMed] [Google Scholar]
  6. Folkman J. What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst. 1990 Jan 3;82(1):4–6. doi: 10.1093/jnci/82.1.4. [DOI] [PubMed] [Google Scholar]
  7. Heuser L. S., Miller F. N. Differential macromolecular leakage from the vasculature of tumors. Cancer. 1986 Feb 1;57(3):461–464. doi: 10.1002/1097-0142(19860201)57:3<461::aid-cncr2820570310>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  8. Matsubara T., Saura R., Hirohata K., Ziff M. Inhibition of human endothelial cell proliferation in vitro and neovascularization in vivo by D-penicillamine. J Clin Invest. 1989 Jan;83(1):158–167. doi: 10.1172/JCI113853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McAuslan B. R., Reilly W. Endothelial cell phagokinesis in response to specific metal ions. Exp Cell Res. 1980 Nov;130(1):147–157. doi: 10.1016/0014-4827(80)90051-8. [DOI] [PubMed] [Google Scholar]
  10. Parke A., Bhattacherjee P., Palmer R. M., Lazarus N. R. Characterization and quantification of copper sulfate-induced vascularization of the rabbit cornea. Am J Pathol. 1988 Jan;130(1):173–178. [PMC free article] [PubMed] [Google Scholar]
  11. Raju K. S., Alessandri G., Gullino P. M. Characterization of a chemoattractant for endothelium induced by angiogenesis effectors. Cancer Res. 1984 Apr;44(4):1579–1584. [PubMed] [Google Scholar]
  12. Reed M. W., Wieman T. J., Schuschke D. A., Tseng M. T., Miller F. N. A comparison of the effects of photodynamic therapy on normal and tumor blood vessels in the rat microcirculation. Radiat Res. 1989 Sep;119(3):542–552. [PubMed] [Google Scholar]
  13. Schuschke D. A., Saari J. T., Ackermann D. M., Miller F. N. Microvascular responses in copper-deficient rats. Am J Physiol. 1989 Nov;257(5 Pt 2):H1607–H1612. doi: 10.1152/ajpheart.1989.257.5.H1607. [DOI] [PubMed] [Google Scholar]
  14. Shing Y. Heparin-copper biaffinity chromatography of fibroblast growth factors. J Biol Chem. 1988 Jun 25;263(18):9059–9062. [PubMed] [Google Scholar]
  15. Zagzag D., Brem S., Robert F. Neovascularization and tumor growth in the rabbit brain. A model for experimental studies of angiogenesis and the blood-brain barrier. Am J Pathol. 1988 May;131(2):361–372. [PMC free article] [PubMed] [Google Scholar]
  16. Ziche M., Jones J., Gullino P. M. Role of prostaglandin E1 and copper in angiogenesis. J Natl Cancer Inst. 1982 Aug;69(2):475–482. [PubMed] [Google Scholar]

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