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. 2007 May 1;7(2):171–178. doi: 10.1111/j.1582-4934.2003.tb00216.x

Mast cells in tissue response to dentistry materials: an adhesive resin, a calcium hydroxide and a glass ionomer cement

R L De Oliveira Mussel 1,3, E De Sá Silva 1, Andréa Monte Alto Costa 2, C A Mandarim‐De‐Lacerda 3,✉
PMCID: PMC6740275  PMID: 12927056

Abstract

Synthetic materials used in dentistry may trigger various inflammatory responses. In order to evaluate biocompatibility, standardized implants of Calcium Hydroxide (CH), Glass Ionomer Cement (GIC) and Light‐activated Dental Adhesive (LDA) were surgically introduced into Wistar rats’back bone. Six (experimental) animal groups, five each, and two Sham (S) groups were studied after 15 and 30 days from surgery. In each animal, the density of mast cells and interstitial fibrosis volume was evaluated by quantitative light microscopy. In addition, the interaction between the disk material and its fibrous capsule was evaluated by scanning electron microscopy. In addition, the interaction between the disk material and its fibrous capsule was evaluated by scanning electron microscopy. The density of mast cells per area (NA[mast cells]) was lower in CH group than in LDA group. GIC group displayed NA[mast cells]) results intermediate between CH and LDA groups (p<0.05). The smallest interstitial fibrosis volume density (VV(f)) was observed in CH group, the in GIC group, whilet he greatest in LDA group. After 30 days, the fibrosis in LDA group was 30% higher than in CH group (p<0.05). In S group, discreet fibrosis restricted to surgical area was present, with few mast cells near the vessels. Significant interaction between fibrous capsule and the surrounding disk material was most evident in CH group. The implanted materials induced mast cell migration, distinc fibrosis development, suggesting that CH is the most biocompatible material among those tested.

Keywords: biocompatibility, mast cell‐ fibrosis, calcium hydroxide cement, glass ionomer cement, dentin adhesive

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