Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1992 Mar 1;282(Pt 2):309–312. doi: 10.1042/bj2820309

The opening of the inositol 1,4,5-trisphosphate-sensitive Ca2+ channel in rat cerebellum is inhibited by caffeine.

G R Brown 1, L G Sayers 1, C J Kirk 1, R H Michell 1, F Michelangeli 1
PMCID: PMC1130779  PMID: 1312323

Abstract

Ins(1,4,5)P3(InsP3)-induced Ca2+ release and [3H]InsP3 binding were measured in rat cerebellar microsomes in the presence or absence of caffeine. The quantal Ca2+ release was shown to occur in an apparently co-operative fashion with a Hill coefficient (h) of 2.2. Half-maximal Ca2+ release was observed at 900 nM-InsP3. Addition of caffeine caused changes both to the concentration of InsP3 required to cause half-maximal Ca2+ release (3.9 microM at 50 mM-caffeine) and to the apparent co-operativity (h = 1.0 at 50 mM-caffeine). Under standard conditions for [3H]InsP3 binding, caffeine had no effect, and it had no effect on InsP3 metabolism. Cyclic AMP also had no effect on the quantal release induced by InsP3. These results are consistent with the view that caffeine affects the opening (Ca2+ release) events rather than the ligand-binding events in the operation of the InsP3-sensitive Ca2+ channel.

Full text

PDF
309

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderson B. H., Volpe P. Distribution of endoplasmic reticulum and calciosome markers in membrane fractions isolated from different regions of the canine brain. Arch Biochem Biophys. 1989 Jul;272(1):162–174. doi: 10.1016/0003-9861(89)90207-5. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J. Calcium oscillations. J Biol Chem. 1990 Jun 15;265(17):9583–9586. [PubMed] [Google Scholar]
  3. Berridge M. J., Irvine R. F. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984 Nov 22;312(5992):315–321. doi: 10.1038/312315a0. [DOI] [PubMed] [Google Scholar]
  4. Cheek T. R., Barry V. A., Berridge M. J., Missiaen L. Bovine adrenal chromaffin cells contain an inositol 1,4,5-trisphosphate-insensitive but caffeine-sensitive Ca2+ store that can be regulated by intraluminal free Ca2+. Biochem J. 1991 May 1;275(Pt 3):697–701. doi: 10.1042/bj2750697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cheek T. R., O'Sullivan A. J., Moreton R. B., Berridge M. J., Burgoyne R. D. The caffeine-sensitive Ca2+ store in bovine adrenal chromaffin cells; an examination of its role in triggering secretion and Ca2+ homeostasis. FEBS Lett. 1990 Jun 18;266(1-2):91–95. doi: 10.1016/0014-5793(90)81514-o. [DOI] [PubMed] [Google Scholar]
  6. Furuichi T., Yoshikawa S., Miyawaki A., Wada K., Maeda N., Mikoshiba K. Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400. Nature. 1989 Nov 2;342(6245):32–38. doi: 10.1038/342032a0. [DOI] [PubMed] [Google Scholar]
  7. Föhr K. J., Scott J., Ahnert-Hilger G., Gratzl M. Characterization of the inositol 1,4,5-trisphosphate-induced calcium release from permeabilized endocrine cells and its inhibition by decavanadate and p-hydroxymercuribenzoate. Biochem J. 1989 Aug 15;262(1):83–89. doi: 10.1042/bj2620083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Imagawa T., Smith J. S., Coronado R., Campbell K. P. Purified ryanodine receptor from skeletal muscle sarcoplasmic reticulum is the Ca2+-permeable pore of the calcium release channel. J Biol Chem. 1987 Dec 5;262(34):16636–16643. [PubMed] [Google Scholar]
  9. Irvine R. F. 'Quantal' Ca2+ release and the control of Ca2+ entry by inositol phosphates--a possible mechanism. FEBS Lett. 1990 Apr 9;263(1):5–9. doi: 10.1016/0014-5793(90)80692-c. [DOI] [PubMed] [Google Scholar]
  10. Maeda N., Kawasaki T., Nakade S., Yokota N., Taguchi T., Kasai M., Mikoshiba K. Structural and functional characterization of inositol 1,4,5-trisphosphate receptor channel from mouse cerebellum. J Biol Chem. 1991 Jan 15;266(2):1109–1116. [PubMed] [Google Scholar]
  11. McPherson P. S., Campbell K. P. Solubilization and biochemical characterization of the high affinity [3H]ryanodine receptor from rabbit brain membranes. J Biol Chem. 1990 Oct 25;265(30):18454–18460. [PubMed] [Google Scholar]
  12. Michelangeli F., Di Virgilio F., Villa A., Podini P., Meldolesi J., Pozzan T. Identification, kinetic properties and intracellular localization of the (Ca(2+)-Mg2+)-ATPase from the intracellular stores of chicken cerebellum. Biochem J. 1991 May 1;275(Pt 3):555–561. doi: 10.1042/bj2750555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Michelangeli F. Fluo-3 an ideal calcium indicator for measuring calcium fluxes in SR and ER. Biochem Soc Trans. 1991 Apr;19(2):183S–183S. doi: 10.1042/bst019183s. [DOI] [PubMed] [Google Scholar]
  14. Muallem S., Pandol S. J., Beeker T. G. Hormone-evoked calcium release from intracellular stores is a quantal process. J Biol Chem. 1989 Jan 5;264(1):205–212. [PubMed] [Google Scholar]
  15. Nelson T. E., Nelson K. E. Intra- and extraluminal sarcoplasmic reticulum membrane regulatory sites for Ca2(+)-induced Ca2+ release. FEBS Lett. 1990 Apr 24;263(2):292–294. doi: 10.1016/0014-5793(90)81396-6. [DOI] [PubMed] [Google Scholar]
  16. Oldershaw K. A., Nunn D. L., Taylor C. W. Quantal Ca2+ mobilization stimulated by inositol 1,4,5-trisphosphate in permeabilized hepatocytes. Biochem J. 1991 Sep 15;278(Pt 3):705–708. doi: 10.1042/bj2780705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Palade P., Dettbarn C., Brunder D., Stein P., Hals G. Pharmacology of calcium release from sarcoplasmic reticulum. J Bioenerg Biomembr. 1989 Apr;21(2):295–320. doi: 10.1007/BF00812074. [DOI] [PubMed] [Google Scholar]
  18. Palade P. Drug-induced Ca2+ release from isolated sarcoplasmic reticulum. I. Use of pyrophosphate to study caffeine-induced Ca2+ release. J Biol Chem. 1987 May 5;262(13):6135–6141. [PubMed] [Google Scholar]
  19. Parker I., Ivorra I. Caffeine inhibits inositol trisphosphate-mediated liberation of intracellular calcium in Xenopus oocytes. J Physiol. 1991 Feb;433:229–240. doi: 10.1113/jphysiol.1991.sp018423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shears S. B. Kinetic consequences of the inhibition by ATP of the metabolism of inositol (1,4,5) trisphosphate and inositol (1,3,4,5) tetrakisphosphate in liver. Different effects upon the 3- and 5-phosphatases. Cell Signal. 1990;2(2):191–195. doi: 10.1016/0898-6568(90)90023-4. [DOI] [PubMed] [Google Scholar]
  21. Shears S. B., Parry J. B., Tang E. K., Irvine R. F., Michell R. H., Kirk C. J. Metabolism of D-myo-inositol 1,3,4,5-tetrakisphosphate by rat liver, including the synthesis of a novel isomer of myo-inositol tetrakisphosphate. Biochem J. 1987 Aug 15;246(1):139–147. doi: 10.1042/bj2460139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Strupish J., Wojcikiewicz R. J., Challiss R. A., Safrany S. T., Willcocks A. L., Potter B. V., Nahorski S. R. Is decavanadate a specific inositol 1,4,5-trisphosphate receptor antagonist? Biochem J. 1991 Jul 1;277(Pt 1):294–294. doi: 10.1042/bj2770294a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tsien R. Y., Pozzan T., Rink T. J. Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J Cell Biol. 1982 Aug;94(2):325–334. doi: 10.1083/jcb.94.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Volpe P., Alderson-Lang B. H., Nickols G. A. Regulation of inositol 1,4,5-trisphosphate-induced Ca2+ release. I. Effect of Mg2+. Am J Physiol. 1990 Jun;258(6 Pt 1):C1077–C1085. doi: 10.1152/ajpcell.1990.258.6.C1077. [DOI] [PubMed] [Google Scholar]
  25. Volpe P., Alderson-Lang B. H. Regulation of inositol 1,4,5-trisphosphate-induced Ca2+ release. II. Effect of cAMP-dependent protein kinase. Am J Physiol. 1990 Jun;258(6 Pt 1):C1086–C1091. doi: 10.1152/ajpcell.1990.258.6.C1086. [DOI] [PubMed] [Google Scholar]
  26. Worley P. F., Baraban J. M., Supattapone S., Wilson V. S., Snyder S. H. Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium. J Biol Chem. 1987 Sep 5;262(25):12132–12136. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES