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. 1992 Jul;174(13):4509–4512. doi: 10.1128/jb.174.13.4509-4512.1992

Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B.

C R Meyer 1, P Ghosh 1, E Remy 1, J Preiss 1
PMCID: PMC206240  PMID: 1320612

Abstract

A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295.

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Selected References

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