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. 1976 Jul;14(1):83–87. doi: 10.1128/iai.14.1.83-87.1976

Adjuvant and mitogenic properties of a supernatant fraction of sonically treated Myobacterium bovis (BCG).

L A Baker, T Sharpton, P Minden, P A Campbell
PMCID: PMC420847  PMID: 133083

Abstract

A water-soluble, oil-free supernatant fraction of sonically treated BCG (BCG-SS) was shown to be an immunological adjuvant and a mitogen. When BCG-SS and sheep erythrocytes (SRBC) were injected intravenously into mice, the plaque-forming cell (PFC) response was 10 times greater than that induced by injection of SRBC alone. Circulating antibody responses to SRBC and to bovine serum albumin were also enhanced by BCG-SS. The in vitro enhancement of PFC and circulating antibody responses did not require mineral oil or exogenous lipids. In vitro PFC responses by normal mouse spleen cells were also greatly increased in the presence of BCG-SS. Anti-theta serum-treated spleen cells from mice that had been lethally irradiated and reconstituted with normal bone marrow cells also gave a higher PFC response to SRBC in the presence of BCG-SS. This suggests that BCG-SS can stimulate B lymphocytes to develop an immune response when T lymphocytes are severely depleted or absent. BCG-SS also stimulated the uptake of 125IUdR by normal spleen cell cultures, indicating that it is a mitogen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azuma I., Kanetsuna F., Taniyama T., Yamamura Y., Hori M. Adjuvant activity of mycobacterial fractions. I. Purification and in vivo adjuvant activity of cell wall skeletons of Mycobacterium bovis BCG, Nocardia asteroides 131 and Corynebacterium diphtheriae PW8. Biken J. 1975 Mar;18(1):1–13. [PubMed] [Google Scholar]
  2. Azuma I., Kishimoto S., Yamamura Y., Petit J. F. Adjuvanticity of mycobacterial cell walls. Jpn J Microbiol. 1971 Mar;15(2):193–197. doi: 10.1111/j.1348-0421.1971.tb00569.x. [DOI] [PubMed] [Google Scholar]
  3. Campbell P. A., Martens B. L., Cooper H. R., McClatchy J. K. Requirement for bone marrow-derived cells in resistance to Listeria. J Immunol. 1974 Apr;112(4):1407–1414. [PubMed] [Google Scholar]
  4. Hawrylko E., Mackaness G. B. Immunopotentiation with BCG. 3. Modulation of the response to a tumor-specific antigen. J Natl Cancer Inst. 1973 Nov;51(5):1677–1682. doi: 10.1093/jnci/51.5.1677. [DOI] [PubMed] [Google Scholar]
  5. Hiu I. J. Water-soluble and lipid-free fraction from BCG with adjuvant and antitumour activity. Nat New Biol. 1972 Aug 23;238(86):241–242. doi: 10.1038/newbio238241a0. [DOI] [PubMed] [Google Scholar]
  6. Mackaness G. B., Auclair D. J., Lagrange P. H. Immunopotentiation with BCG. I. Immune response to different strains and preparations. J Natl Cancer Inst. 1973 Nov;51(5):1655–1667. doi: 10.1093/jnci/51.5.1655. [DOI] [PubMed] [Google Scholar]
  7. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  8. Meyer T. J., Azuma I., Ribi E. E. Biologically active components from mycobacterial cell walls. III. Production of experimental allergic encephalomyelitis in guinea-pigs. Immunology. 1975 Feb;28(2):219–229. [PMC free article] [PubMed] [Google Scholar]
  9. Meyer T. J., Ribi E. E., Azuma I., Zbar B. Biologically active components from mycobacterial cell walls. II. Suppression and regression of strain-2 guinea pig hepatoma. J Natl Cancer Inst. 1974 Jan;52(1):103–111. doi: 10.1093/jnci/52.1.103. [DOI] [PubMed] [Google Scholar]
  10. Miller T. E., Mackaness G. B., Lagrange P. H. Immunopotentiation with BCG. II. Modulation of the response to sheep red blood cells. J Natl Cancer Inst. 1973 Nov;51(5):1669–1676. doi: 10.1093/jnci/51.5.1669. [DOI] [PubMed] [Google Scholar]
  11. Minden P., Anthony B. F., Farr R. S. A comparison of seven procedures to detect the primary binding of antigen by antibody. J Immunol. 1969 Apr;102(4):832–841. [PubMed] [Google Scholar]
  12. Minden P., Farr R. S. Binding between components of the tubercle bacillus and humoral antibodies. J Exp Med. 1969 Nov 1;130(5):931–954. doi: 10.1084/jem.130.5.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Minden P., Farr R. S., Trembath J. Some variable controls for accurate heterologous anti-immunoglobulin (HAI) tests. Immunochemistry. 1975 Jul;12(6-7):477–479. doi: 10.1016/0019-2791(75)90069-5. [DOI] [PubMed] [Google Scholar]
  14. Minden P., McClatchy J. K., Wainberg M., Weiss D. W. Shared antigens between Mycobacterium bovis (BCG) and neoplastic cells. J Natl Cancer Inst. 1974 Nov;53(5):1325–1331. doi: 10.1093/jnci/53.5.1325. [DOI] [PubMed] [Google Scholar]
  15. Minden P., Sharpton T. R., McClatchy J. K. Shared antigens between human malignant melanoma cells and Mycobacterium bovis (BCG). J Immunol. 1976 May;116(5):1407–1414. [PubMed] [Google Scholar]
  16. Minden P., Wainberg M., Weiss D. W. Protection against guinea pig hepatomas by pretreatment with subcelluar fractions of Mycobacterium bovis (BCG). J Natl Cancer Inst. 1974 May;52(5):1643–1645. doi: 10.1093/jnci/52.5.1643. [DOI] [PubMed] [Google Scholar]
  17. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mokyr M. B., Mitchell M. S. Activation of lymphoid cells by BCG in vitro. Cell Immunol. 1975 Feb;15(2):264–273. doi: 10.1016/0008-8749(75)90005-2. [DOI] [PubMed] [Google Scholar]
  19. Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
  20. Yashphe D. J., Weiss D. W. Modulation of the immune response by a methanol-insoluble fraction of attenuated tubercle bacilli (BCG). I. Primary and secondary responses to sheep red blood cells and T2 phage. Clin Exp Immunol. 1970 Aug;7(2):269–281. [PMC free article] [PubMed] [Google Scholar]
  21. Zbar B., Minden P., McClatchy J. K., Rapp H. J. Prevention of tumor growth after intradermal injection of BCG extracts: a comparison of results in strain-2 guinea pigs from the National Institutes of Health and from the National Jewish Hospital and Research Center. J Natl Cancer Inst. 1976 Feb;56(2):443–444. doi: 10.1093/jnci/56.2.443. [DOI] [PubMed] [Google Scholar]
  22. Zbar B., Rapp H. J., Ribi E. E. Tumor suppression by cell walls of mycobacterium bovis attached to oil droplets. J Natl Cancer Inst. 1972 Mar;48(3):831–835. [PubMed] [Google Scholar]

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