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. 1961 Jul 1;114(1):13–28. doi: 10.1084/jem.114.1.13

IMMUNOCYTOLOGICAL EFFECTS OF VARIED INOCULA OF INFLUENZA VIRUS

Barbara K Watson 1
PMCID: PMC2137440  PMID: 13783417

Abstract

Variation in the amount and quality of influenza virus injected into the amniotic sac of the chick embryo led to differences not only in the yield of virus but also in the immunofluorescent cytology of the infection. The production of infectious virus was associated with a predominance of cells in which the virus antigens were first detected along the cell surface in contact with the amniotic fluid, later deeper in the cytoplasm, and finally, though to a lesser extent, in the nucleus. When the virus yield was primarily non-infectious hemagglutinin the virus antigens appeared in reverse sequence; i.e., nucleus first, then adjacent cytoplasm, and ultimately throughout the entire cell. Under conditions of "autointerference," the immunofluorescence seen in some of the cells failed to progress beyond the nuclear or early cytoplasmic stage, while many other cells remained unreactive throughout the experimental period. With the largest dose used a pale intranuclear reaction was localized 1 hour after injection but the embryo died shortly thereafter.

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Selected References

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  1. ADA G. L., PERRY B. T. Studies on the soluble complement fixing antigens of influenza virus. 3. The nature of the antigens. Aust J Exp Biol Med Sci. 1954 Apr;32(2):177–185. doi: 10.1038/icb.1954.20. [DOI] [PubMed] [Google Scholar]
  2. BERNKOPF H. Study of infectivity and hemagglutination of influenza virus in deembryonated eggs. J Immunol. 1950 Nov;65(5):571–583. [PubMed] [Google Scholar]
  3. BREITENFELD P. M., SCHAFER W. The formation of fowl plague virus antigens in infected cells, as studied with fluorescent antibodies. Virology. 1957 Oct;4(2):328–345. doi: 10.1016/0042-6822(57)90067-3. [DOI] [PubMed] [Google Scholar]
  4. COONS A. H. Fluorescent antibody methods. Gen Cytochem Methods. 1958;1:399–422. [PubMed] [Google Scholar]
  5. DEIBEL R., HOTCHIN J. E. Quantitative applications of fluorescent antibody technique to influenza-virus-infected cell cultures. Virology. 1959 Jul;8(3):367–380. doi: 10.1016/0042-6822(59)90036-4. [DOI] [PubMed] [Google Scholar]
  6. FRANKLIN R. M., BREITENFELD P. M. The abortive infection of Earle's L-cells by fowl plague virus. Virology. 1959 Jul;8(3):293–307. doi: 10.1016/0042-6822(59)90031-5. [DOI] [PubMed] [Google Scholar]
  7. FRISCH-NIGGEMEYER W., HOYLE L. The nucleic acid and carbohydrate content of influenza virus A and of virus fractions produced by ether disintegration. J Hyg (Lond) 1956 Jun;54(2):201–212. doi: 10.1017/s0022172400044466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FULTON F., ISAACS A. Influenza virus multiplication in the chick chorioallantoic membrane. J Gen Microbiol. 1953 Aug;9(1):119–131. doi: 10.1099/00221287-9-1-119. [DOI] [PubMed] [Google Scholar]
  9. HENLE G., GIRARDI A., HENLE W. A non-transmissible cytopathogenic effect of influenza virus in tissue culture accompanied by formation of non-infectious hemagglutinins. J Exp Med. 1955 Jan 1;101(1):25–41. doi: 10.1084/jem.101.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HENLE W., LIEF F. S., PAUCKER K. Studies on the soluble antigen of influenza virus. IV. Fractionation of elementary bodies labeled with radioactive phosphorus. Virology. 1956 Dec;2(6):798–810. doi: 10.1016/0042-6822(56)90059-9. [DOI] [PubMed] [Google Scholar]
  11. HENLE W., LIEF F. S. Studies on the soluble antigen of influenza virus. I. The release of S antigen from elementary bodies by treatment with ether. Virology. 1956 Dec;2(6):753–771. doi: 10.1016/0042-6822(56)90056-3. [DOI] [PubMed] [Google Scholar]
  12. HENLE W., LIEF F. S. Studies on the soluble antigen of influenza virus. II. A comparison of the effects of sonic vibration and ether treatment of elementary bodies. Virology. 1956 Dec;2(6):772–781. doi: 10.1016/0042-6822(56)90057-5. [DOI] [PubMed] [Google Scholar]
  13. HENLE W., LIU O. C., PAUCKER K., LIEF F. S. Studies on host-virus interactions in the chick embryo-influenza virus system. XIV. The relation between tissue-bound and liberated virus materials under various conditions of infection. J Exp Med. 1956 Jun 1;103(6):799–822. doi: 10.1084/jem.103.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HOYLE L., JOLLES B., MITCHELL R. G. The incorporation of radioactive phosphorus in the influenza virus and its distribution in serologically active virus fractions. J Hyg (Lond) 1954 Mar;52(1):119–127. doi: 10.1017/s0022172400027315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HOYLE L. Structure of the influenza virus; the relation between biological activity and chemical structure of virus fractions. J Hyg (Lond) 1952 Jun;50(2):229–245. doi: 10.1017/s0022172400019562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. HOYLE L. The growth cycle of influenza virus A; a study of the relations between virus, soluble antigen and host cell in fertile eggs inoculated with influenza virus. Br J Exp Pathol. 1948 Oct;29(5):390–399. [PMC free article] [PubMed] [Google Scholar]
  17. Knight C. A. PRECIPITIN REACTIONS OF HIGHLY PURIFIED INFLUENZA VIRUSES AND RELATED MATERIALS. J Exp Med. 1946 Mar 31;83(4):281–294. [PMC free article] [PubMed] [Google Scholar]
  18. LIU O. C., HENLE W. Studies on host-virus interactions in the chick embryo-influenza virus system. IV. The role of inhibitors of hemagglutination in the evaluation of viral multiplication. J Exp Med. 1951 Oct;94(4):269–289. doi: 10.1084/jem.94.4.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. PEREIRA H. G., ALLISON A. C., BALFOUR B. Multiplication of adenovirus type 5 studied by infectivity titrations and by the fluorescent antibody technique. Virology. 1959 Mar;7(3):300–314. doi: 10.1016/0042-6822(59)90200-4. [DOI] [PubMed] [Google Scholar]
  20. SCHLESINGER R. W. Incomplete growth cycle of influenza virus in mouse brain. Proc Soc Exp Biol Med. 1950 Jul;74(3):541–548. doi: 10.3181/00379727-74-17966. [DOI] [PubMed] [Google Scholar]
  21. SMITH W., BELYAVIN G., SHEFFIELD F. W. The host-tissue component of influenza viruses. Proc R Soc Lond B Biol Sci. 1955 May 17;143(913):504–522. doi: 10.1098/rspb.1955.0026. [DOI] [PubMed] [Google Scholar]
  22. VON MAGNUS P. Propagation of the PR8 strain of influenza A virus in chick embryos. IV. Studies on the factors involved in the formation of incomplete virus upon serial passage of undiluted virus. Acta Pathol Microbiol Scand. 1952;30(3-4):311–335. [PubMed] [Google Scholar]
  23. WATSON B. K., COONS A. H. Studies of influenza virus infection in the chick embryo using fluorescent antibody. J Exp Med. 1954 May 1;99(5):419–428. doi: 10.1084/jem.99.5.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WHEELOCK E. F., TAMM I. Mitosis and division in HeLa cells infected with influenza or Newcastle disease virus. Virology. 1959 Aug;8:532–536. doi: 10.1016/0042-6822(59)90056-x. [DOI] [PubMed] [Google Scholar]

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