Abstract
Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced dye; (c) lipid adsorption of formazan. The association of formazan- or iron hematoxylin-stained mitochondria with lipid droplets within the cells was investigated, as was also the influence of formalin fixation, with and without cold acetone pretreatment, on mitochondrial morphology and enzymatic staining. Data from these studies appear to indicate that TNBT is more suitable than NBT for use as a cytochemical reagent in oxidative and/or dehydrogenase enzyme histochemistry and cytochemistry.
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- NACHLAS M. M., WALKER D. G., SELIGMAN A. M. A histochemical method for the demonstration of diphosphopyridine nucleotide diaphorase. J Biophys Biochem Cytol. 1958 Jan 25;4(1):29–38. doi: 10.1083/jcb.4.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NACHLAS M. M., WALKER D. G., SELIGMAN A. M. The histochemical localization of triphosphopyridine nucleotide diaphorase. J Biophys Biochem Cytol. 1958 Jul 25;4(4):467–474. doi: 10.1083/jcb.4.4.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOVIKOFF A. B., SHIN W. Y., DRUCKER J. Cold acetone fixation for enzyme localization in frozen sections. J Histochem Cytochem. 1960 Jan;8:37–40. doi: 10.1177/8.1.37. [DOI] [PubMed] [Google Scholar]
- NOVIKOFF A. B., SHIN W. Y., DRUCKER J. Mitochondrial localization of oxidative enzymes: staining results with two tetrazolium salts. J Biophys Biochem Cytol. 1961 Jan;9:47–61. doi: 10.1083/jcb.9.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PEARSE A. G., HESS R. Substantivity and other factors responsible for formazan patterns in dehydrogenase histochemistry. Experientia. 1961 Mar 15;17:136–141. doi: 10.1007/BF02160833. [DOI] [PubMed] [Google Scholar]
- ROSA C. G., TSOU K. C. Use of tetrazolium compounds in oxidative enzyme histo- and cytochemistry. Nature. 1961 Dec 9;192:990–991. doi: 10.1038/192990a0. [DOI] [PubMed] [Google Scholar]
- SABATINI D. D., DE ROBERTIS E. D. Ultrastructural zonation of adrenocortex in the rat. J Biophys Biochem Cytol. 1961 Jan;9:105–119. doi: 10.1083/jcb.9.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WALKER D. G., SELIGMAN A. M. Formalin fixation in the cytochemical demonstration of succinic dehydrogenase of mitochondria. J Biophys Biochem Cytol. 1961 Feb;9:415–427. doi: 10.1083/jcb.9.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATTENBERG L. W. Microscopic histochemical demonstration of steroid-3 beta-ol dehydrogenase in tissue sections. J Histochem Cytochem. 1958 Jul;6(4):225–232. doi: 10.1177/6.4.225. [DOI] [PubMed] [Google Scholar]