Abstract
Gametophytic and sporophytic tissues of Chondrus crispus (Stackhouse) cultured in vitro were labeled with 35S and 14C. The major sulfated polysccharides isolated from the two karyotypes were characterized by KCI fractionation, immunoprecipitation, and infrared spectroscopy. Reproducibility of data has been demonstrated by an experiment using gametophytic T4 strain with five replicates per time point. The rate of sulfate uptake was similar in haploid and diploid plants from a given area cultured for a similar time. Cultures from different sources cultured for different times showed different uptake and incorporation levels. Although sulfate uptake did not appear to be karyotype-related, the pattern of incorporation of 35S and 14C into polysaccharides was ploidy-specific.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Loewus F., Wagner G. The Incorporation of S-Labeled Sulfate into Carrageenan in Chondrus crispus. Plant Physiol. 1971 Sep;48(3):373–375. doi: 10.1104/pp.48.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novotny A. M., Forman M. The relationship between changes in cell wall composition and the establishment of polarity in Fucus embryos. Dev Biol. 1974 Sep;40(1):162–173. doi: 10.1016/0012-1606(74)90116-x. [DOI] [PubMed] [Google Scholar]
- Rees D. A. Structure, conformation, and mechanism in the formation of polysaccharide gels and networks. Adv Carbohydr Chem Biochem. 1969;24:267–332. doi: 10.1016/s0065-2318(08)60352-2. [DOI] [PubMed] [Google Scholar]