Abstract
Incubation of peeled oat Avena sativa L. var Victory leaf segments on media of pH 5.0 or below leads to a rapid and massive increase in the titer of putrescine while incubation at pH values above 5.0 causes little or no change. The low pH effect is independent of the buffer system employed. Putrescine levels rise within 3 hours and reach their peak 8 to 9 hours after acidification. At this time, putrescine titer is eight times greater at pH 3.5 than at 6.0. None of the other polyamines shows a response to changes in external pH. The increase in putrescine is blocked by the addition of cycloheximide or dl-α-difluoromethylarginine, a specific inhibitor of the putrescine biosynthetic enzyme, arginine decarboxylase. In one experiment, arginine decarboxylase activity was 110% greater at pH 4.0 than at 6.0 after a 4-hour incubation, although the average increase over many experiments was 47%. The activity of the other possible putrescine biosynthetic enzyme, ornithine decarboxylase, falls throughout the incubation period and is virtually equal at pH 4.0 and 6.0.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Altman A., Friedman R., Levin N. Arginine and ornithine decarboxylases, the polyamine biosynthetic enzymes of mung bean seedlings. Plant Physiol. 1982 Apr;69(4):876–879. doi: 10.1104/pp.69.4.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atmar V. J., Daniels G. R., Kuehn G. D. Polyamine stimulation of phosphorylation of nonhistone acidic protein in nuclei and nucleoli from Physarum polycephalum. Eur J Biochem. 1978 Sep 15;90(1):29–37. doi: 10.1111/j.1432-1033.1978.tb12571.x. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Cohen E., Arad S. M., Heimer Y. M., Mizrahi Y. Participation of ornithine decarboxylase in early stages of tomato fruit development. Plant Physiol. 1982 Aug;70(2):540–543. doi: 10.1104/pp.70.2.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Flores H. E., Galston A. W. Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol. 1982 Mar;69(3):701–706. doi: 10.1104/pp.69.3.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Flores H. E., Galston A. W. Polyamines and plant stress: activation of putrescine biosynthesis by osmotic shock. Science. 1982 Sep 24;217(4566):1259–1261. doi: 10.1126/science.217.4566.1259. [DOI] [PubMed] [Google Scholar]
- Good N. E., Winget G. D., Winter W., Connolly T. N., Izawa S., Singh R. M. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467–477. doi: 10.1021/bi00866a011. [DOI] [PubMed] [Google Scholar]
- Kallio A., McCann P. P., Bey P. DL-alpha-(Difluoromethyl)arginine: a potent enzyme-activated irreversible inhibitor of bacterial decarboxylases. Biochemistry. 1981 May 26;20(11):3163–3168. doi: 10.1021/bi00514a027. [DOI] [PubMed] [Google Scholar]
- Kaur-Sawhney R., Altman A., Galston A. W. Dual Mechanisms in Polyamine-mediated Control of Ribonuclease Activity in Oat Leaf Protoplasts. Plant Physiol. 1978 Jul;62(1):158–160. doi: 10.1104/pp.62.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaur-Sawhney R., Flores H. E., Galston A. W. Polyamine-induced DNA Synthesis and Mitosis in Oat Leaf Protoplasts. Plant Physiol. 1980 Feb;65(2):368–371. doi: 10.1104/pp.65.2.368. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith T. A. The occurrence, metabolism and functions of amines in plants. Biol Rev Camb Philos Soc. 1971 May;46(2):201–241. doi: 10.1111/j.1469-185x.1971.tb01182.x. [DOI] [PubMed] [Google Scholar]