Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1987 Oct;85(2):469–473. doi: 10.1104/pp.85.2.469

Induction of Sesquiterpenoid Biosynthesis in Tobacco Cell Suspension Cultures by Fungal Elicitor 1

Joseph Chappell 1, Ross Nable 1,2
PMCID: PMC1054280  PMID: 16665722

Abstract

Large amounts of the sesquiterpenoid capsidiol accumulated in the media of tobacco (Nicotiana tabacum L. cv KY14) cell suspension cultures upon addition of fungal elicitor. Capsidiol accumulation was proportional to the amount of elicitor added. The accumulation of capsidiol was preceded by a transient increase in the capsidiol de novo synthesis rate as measured by the incorporation of exogenous [14C]acetate. Changes in 3-hydroxy-3-methylglutaryl-CoA reductase activity (HMGR; EC 1.1.1.34), an enzyme of general isoprenoid metabolism, paralleled the changes in [14C]acetate incorporation into capsidiol. Incubation of the cell cultures with mevinolin, a potent in vitro inhibitor of the tobacco HMGR enzyme activity, inhibited the elicitor-induced capsidiol accumulation in a concentration dependent manner. [14C]Acetate incorporation into capsidiol was likewise inhibited by mevinolin treatment. Unexpectedly, [3H] mevalonate incorporation into capsidiol was also partially inhibited by mevinolin, suggesting that mevinolin may effect secondary sites of sesquiterpenoid biosynthesis in vivo beyond HMGR. The data indicated the importance of the induced HMGR activity for capsidiol production in elicitor-treated tobacco cell suspension cultures.

Full text

PDF
469

Images in this article

471Fig. 1
on p.471
471Fig. 2
on p.471

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts A. W., Chen J., Kuron G., Hunt V., Huff J., Hoffman C., Rothrock J., Lopez M., Joshua H., Harris E. Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3957–3961. doi: 10.1073/pnas.77.7.3957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ayers A. R., Ebel J., Valent B., Albersheim P. Host-Pathogen Interactions: X. Fractionation and Biological Activity of an Elicitor Isolated from the Mycelial Walls of Phytophthora megasperma var. sojae. Plant Physiol. 1976 May;57(5):760–765. doi: 10.1104/pp.57.5.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bach T. J., Rogers D. H., Rudney H. Detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme A reductase from radish seedlings. Eur J Biochem. 1986 Jan 2;154(1):103–111. doi: 10.1111/j.1432-1033.1986.tb09364.x. [DOI] [PubMed] [Google Scholar]
  4. Chappell J., Beevers H. Transport of dicarboxylic acids in castor bean mitochondria. Plant Physiol. 1983 Jun;72(2):434–440. doi: 10.1104/pp.72.2.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dudley M. W., Dueber M. T., West C. A. Biosynthesis of the Macrocyclic Diterpene Casbene in Castor Bean (Ricinus communis L.) Seedlings : Changes in Enzyme Levels Induced by Fungal Infection and Intracellular Localization of the Pathway. Plant Physiol. 1986 Jun;81(2):335–342. doi: 10.1104/pp.81.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Keene C. K., Wagner G. J. Direct demonstration of duvatrienediol biosynthesis in glandular heads of tobacco trichomes. Plant Physiol. 1985 Dec;79(4):1026–1032. doi: 10.1104/pp.79.4.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kombrink E., Hahlbrock K. Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions. Plant Physiol. 1986 May;81(1):216–221. doi: 10.1104/pp.81.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kuhn D. N., Chappell J., Boudet A., Hahlbrock K. Induction of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase mRNAs in cultured plant cells by UV light or fungal elicitor. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1102–1106. doi: 10.1073/pnas.81.4.1102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Robinson D. R., West C. A. Biosynthesis of cyclic diterpenes in extracts from seedlings of Ricinus communis L. I. Identification of diterpene hydrocarbons formed from mevalonate. Biochemistry. 1970 Jan 6;9(1):70–79. doi: 10.1021/bi00803a010. [DOI] [PubMed] [Google Scholar]
  10. Stermer B. A., Bostock R. M. Involvement of 3-hydroxy-3-methylglutaryl coenzyme a reductase in the regulation of sesquiterpenoid phytoalexin synthesis in potato. Plant Physiol. 1987 Jun;84(2):404–408. doi: 10.1104/pp.84.2.404. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES