Abstract
Red light (R) stimulates germination in Scots pine seed (Pinus sylvestris L.). The response is far red (FR) reversible. The dynamics of cytokinin changes following light treatment was investigated. Extracts were purified by immunoaffinity and high performance liquid chromatography. N6-(Δ2-Isopentenyl) adenosine (iPA) and trans-zeatin riboside (ZR) were quantified by both UV-absorbance of high performance liquid chromatography peaks and by enzyme-linked immunosorbent assay. Identification of iPA was accomplished by gas chromatography-mass spectrometry. Levels of cytokinins were low in seeds imbibed in the dark. Exposure of seeds imbibed in the dark for 5 hours to R for 15 minutes induced a strong, immediate but transitory increase in iPA content. This increase was not observed when the R treatment was followed by 10 minutes of FR or by storage in darkness before extraction. No ZR was detected during the first 8 hours of imbibition in any treatment. Addition of iPA via acetone enhanced seed germination in the dark. The results suggest that iPA may be involved in the R-mediated release of dormancy of Scots pine seed.
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Selected References
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- Hocart C. H., Wong O. C., Letham D. S., Tay S. A., MacLeod J. K. Mass spectrometry and chromatography of t-butyldimethylsilyl derivatives of cytokinin bases. Anal Biochem. 1986 Feb 15;153(1):85–96. doi: 10.1016/0003-2697(86)90065-5. [DOI] [PubMed] [Google Scholar]
- Miller C. O. Similarity of Some Kinetin and Red Light Effects. Plant Physiol. 1956 Jul;31(4):318–319. doi: 10.1104/pp.31.4.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tillberg E. Indoleacetic Acid Levels in Phaseolus, Zea, and Pincus during Seed Germination. Plant Physiol. 1977 Aug;60(2):317–319. doi: 10.1104/pp.60.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]