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. 1990 Oct;94(2):641–646. doi: 10.1104/pp.94.2.641

Changes in Properties of Barley Leaf Mitochondria Isolated from NaCl-Treated Plants

Yves Jolivet 1, Jean-Claude Pireaux 1, Pierre Dizengremel 1
PMCID: PMC1077280  PMID: 16667760

Abstract

Treatment of barley (Hordeum vulgare) seedlings with 400 millimolar NaCl for 3 days resulted in a reduction in plant growth and an increase in the leaf content in ions (K+ + Na+) and proline. Purified mitochondria were successfully isolated from barley leaves. Good oxidative and phosphorylative properties were observed with malate as substrate. Malate-dependent electron transport was found to be only partly inhibited by cyanide, the remaining oxygen uptake being SHAM sensitive. The properties of mitochondria from NaCl-treated barley were modified. The efficiency of phosphorylation was diminished with only a slight decrease in the oxidation rates. In both isolated mitochondria and whole leaf tissue of treated plants, the lower respiration rate was due to a lower cytochrome pathway activity. In mitochondria, the activity of the alternative pathway was not modified by salt treatment, whereas this activity was increased in whole leaf tissue. The possible participation of the alternative pathway in response to salt stress will be discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aslam M., Huffaker R. C., Rains D. W. Early effects of salinity on nitrate assimilation in barley seedlings. Plant Physiol. 1984 Oct;76(2):321–325. doi: 10.1104/pp.76.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Azcón-Bieto J., Lambers H., Day D. A. Effect of photosynthesis and carbohydrate status on respiratory rates and the involvement of the alternative pathway in leaf respiration. Plant Physiol. 1983 Jul;72(3):598–603. doi: 10.1104/pp.72.3.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. I. The steady states of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem. 1973 May 25;248(10):3441–3445. [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  6. Criddle R. S., Hansen L. D., Breidenbach R. W., Ward M. R., Huffaker R. C. Effects of NaCl on metabolic heat evolution rates by barley roots. Plant Physiol. 1989;90:53–58. doi: 10.1104/pp.90.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Day D. A., Moore A. L., Dry I. B., Wiskich J. T., Azcon-Bieto J. Regulation of nonphosphorylating electron transport pathways in soybean cotyledon mitochondria and its implications for fat metabolism. Plant Physiol. 1988 Apr;86(4):1199–1204. doi: 10.1104/pp.86.4.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Douce R., Bonner W. D., Jr Oxalacetate control of Krebs cycle oxidations in purified plant mitochondria. Biochem Biophys Res Commun. 1972 May 12;47(3):619–624. doi: 10.1016/0006-291x(72)90923-0. [DOI] [PubMed] [Google Scholar]
  9. Douce R., Christensen E. L., Bonner W. D., Jr Preparation of intaintact plant mitochondria. Biochim Biophys Acta. 1972 Aug 17;275(2):148–160. doi: 10.1016/0005-2728(72)90035-7. [DOI] [PubMed] [Google Scholar]
  10. Gardeström P., Edwards G. E. Isolation of Mitochondria from Leaf Tissue of Panicum miliaceum, a NAD-Malic Enzyme Type C(4) Plant. Plant Physiol. 1983 Jan;71(1):24–29. doi: 10.1104/pp.71.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelly B. M., Wiskich J. T. Respiration of Mitochondria Isolated from Leaves and Protoplasts of Avena sativa. Plant Physiol. 1988 Jul;87(3):705–710. doi: 10.1104/pp.87.3.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Livne A., Levin N. Tissue Respiration and Mitochondrial Oxidative Phosphorylation of NaCl-Treated Pea Seedlings. Plant Physiol. 1967 Mar;42(3):407–414. doi: 10.1104/pp.42.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ramagopal S. Salinity stress induced tissue-specific proteins in barley seedlings. Plant Physiol. 1987 Jun;84(2):324–331. doi: 10.1104/pp.84.2.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schonbaum G. R., Bonner W. D., Jr, Storey B. T., Bahr J. T. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 1971 Jan;47(1):124–128. doi: 10.1104/pp.47.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schwitzguebel J. P., Siegenthaler P. A. Purification of peroxisomes and mitochondria from spinach leaf by percoll gradient centrifugation. Plant Physiol. 1984 Jul;75(3):670–674. doi: 10.1104/pp.75.3.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Voetberg G., Stewart C. R. Steady state proline levels in salt-shocked barley leaves. Plant Physiol. 1984 Nov;76(3):567–570. doi: 10.1104/pp.76.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]

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