Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1992 Jun;99(2):365–367. doi: 10.1104/pp.99.2.365

Electrofusion and Electroporation of Plants

Sally L Van Wert 1,2, James A Saunders 1,2
PMCID: PMC1080468  PMID: 16668891

Abstract

The utilization of electrofusion and electroporation techniques has had a major impact on the genetic manipulation of plants within the last decade. This review of the development of electrofusion and electroporation, as it applies to plants, highlights major developmental aspects of this technology. These include mechanisms for cell fusion, molecular exchange, and parameters that affect the efficiency of fusion and electroporation.

Full text

PDF
365

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bates G. W., Carle S. A., Piastuch W. C. Linear DNA introduced into carrot protoplasts by electroporation undergoes ligation and recircularization. Plant Mol Biol. 1990 Jun;14(6):899–908. doi: 10.1007/BF00019388. [DOI] [PubMed] [Google Scholar]
  2. Callis J., Fromm M., Walbot V. Expression of mRNA electroporated into plant and animal cells. Nucleic Acids Res. 1987 Jul 24;15(14):5823–5831. doi: 10.1093/nar/15.14.5823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dekeyser R. A., Claes B., De Rycke RMU., Habets M. E., Van Montagu M. C., Caplan A. B. Transient Gene Expression in Intact and Organized Rice Tissues. Plant Cell. 1990 Jul;2(7):591–602. doi: 10.1105/tpc.2.7.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dimitrov D. S., Sowers A. E. Membrane electroporation--fast molecular exchange by electroosmosis. Biochim Biophys Acta. 1990 Mar;1022(3):381–392. doi: 10.1016/0005-2736(90)90289-z. [DOI] [PubMed] [Google Scholar]
  5. Fromm M., Taylor L. P., Walbot V. Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5824–5828. doi: 10.1073/pnas.82.17.5824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gallie D. R., Lucas W. J., Walbot V. Visualizing mRNA expression in plant protoplasts: factors influencing efficient mRNA uptake and translation. Plant Cell. 1989 Mar;1(3):301–311. doi: 10.1105/tpc.1.3.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morikawa H., Iida A., Matsui C., Ikegami M., Yamada Y. Gene transfer into intact plant cells by electroinjection through cell walls and membranes. Gene. 1986;41(1):121–124. doi: 10.1016/0378-1119(86)90274-x. [DOI] [PubMed] [Google Scholar]
  8. Potter H., Weir L., Leder P. Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7161–7165. doi: 10.1073/pnas.81.22.7161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rhodes C. A., Pierce D. A., Mettler I. J., Mascarenhas D., Detmer J. J. Genetically transformed maize plants from protoplasts. Science. 1988 Apr 8;240(4849):204–207. doi: 10.1126/science.2832947. [DOI] [PubMed] [Google Scholar]
  10. Rodenburg K. W., de Groot M. J., Schilperoort R. A., Hooykaas P. J. Single-stranded DNA used as an efficient new vehicle for transformation of plant protoplasts. Plant Mol Biol. 1989 Dec;13(6):711–719. doi: 10.1007/BF00016026. [DOI] [PubMed] [Google Scholar]
  11. Saunders J. A., Smith C. R., Kaper J. M. Effects of electroporation pulse wave on the incorporation of viral RNA into tobacco protoplasts. Biotechniques. 1989 Nov-Dec;7(10):1124–1131. [PubMed] [Google Scholar]
  12. Wong T. K., Neumann E. Electric field mediated gene transfer. Biochem Biophys Res Commun. 1982 Jul 30;107(2):584–587. doi: 10.1016/0006-291x(82)91531-5. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES