Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1968 Jul;2(7):738–744. doi: 10.1128/jvi.2.7.738-744.1968

Physicochemical Properties of Tipula Iridescent Virus 1

J Kalmakoff 1, J H Tremaine 1
PMCID: PMC375682  PMID: 16789085

Abstract

The molecular weight of Tipula iridescent virus, based on sedimentation and diffusion coefficients, was 5.51 × 108, with hydration of 0.57 g of water per g of virus. Deoxyribonucleic acid content, based on total inorganic phosphorus liberated, was 19 ± 0.2%. At 260 mμ, the virus gave an uncorrected absorbance of 18.2 cm2/mg of virus and a light-scattering corrected absorbance of 9.8 cm2/mg of virus. Amino acid analyses of the virus protein revealed a remarkable similarity to Sericesthis iridescent virus. The possibility is discussed that the four iridescent insect viruses reported to date bear a strain relationship.

Full text

PDF
738

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON A. C., BURKE D. C. The nucleic acid contents of viruses. J Gen Microbiol. 1962 Feb;27:181–194. doi: 10.1099/00221287-27-2-181. [DOI] [PubMed] [Google Scholar]
  2. Arber W. Host-controlled modification of bacteriophage. Annu Rev Microbiol. 1965;19:365–378. doi: 10.1146/annurev.mi.19.100165.002053. [DOI] [PubMed] [Google Scholar]
  3. Bellett A. J. Some properties of deoxyribonucleic acid preparations from Chilo, Sericesthis and Tipula iridescent viruses. J Mol Biol. 1967 May 14;25(3):425–432. doi: 10.1016/0022-2836(67)90195-7. [DOI] [PubMed] [Google Scholar]
  4. Clark T. B., Kellen W. R., Lum P. T. A mosquito iridescent virus (MIV) from Aedes taeniorhynchus (Wiedemann). J Invertebr Pathol. 1965 Dec;7(4):519–521. doi: 10.1016/0022-2011(65)90133-3. [DOI] [PubMed] [Google Scholar]
  5. GOSTING L. J. Measurement and interpretation of diffusion coefficients of proteins. Adv Protein Chem. 1956;11:429–554. doi: 10.1016/s0065-3233(08)60425-8. [DOI] [PubMed] [Google Scholar]
  6. Harrison B. D., Klug A. Relation between length and sedimentation coefficient for particles of tobacco rattle viruses. Virology. 1966 Dec;30(4):738–740. doi: 10.1016/0042-6822(66)90179-6. [DOI] [PubMed] [Google Scholar]
  7. Hosaka Y. A criterion for evaluating the number of capsomeres of icosahedral capsids. Biochim Biophys Acta. 1965 Jun 15;104(1):261–273. doi: 10.1016/0304-4165(65)90243-6. [DOI] [PubMed] [Google Scholar]
  8. KLUG A., FRANKLIN R. E., HUMPHREYS-OWEN S. P. The crystal structure of Tipula iridescent virus as determined by Bragg reflection of visible light. Biochim Biophys Acta. 1959 Mar;32(1):203–219. doi: 10.1016/0006-3002(59)90570-0. [DOI] [PubMed] [Google Scholar]
  9. Kalmakoff J., Tremaine J. H. Some physical and chemical properties of carnation ringspot virus. Virology. 1967 Sep;33(1):10–16. doi: 10.1016/0042-6822(67)90088-8. [DOI] [PubMed] [Google Scholar]
  10. LAUFFER M. A., BENDET I. J. The hydration of viruses. Adv Virus Res. 1954;2:241–287. doi: 10.1016/s0065-3527(08)60534-5. [DOI] [PubMed] [Google Scholar]
  11. SMITH K. M. A study of the early stages of infection with the Tipula iridescent virus. Parasitology. 1958 Nov;48(3-4):459–462. doi: 10.1017/s0031182000021405. [DOI] [PubMed] [Google Scholar]
  12. THOMAS R. S. The chemical composition and particle weight of Tipula iridescent virus. Virology. 1961 Jun;14:240–252. doi: 10.1016/0042-6822(61)90199-4. [DOI] [PubMed] [Google Scholar]
  13. Weber F. N., Jr, Kupke D. W., Beams J. W. Molecular Weight: Measurements with Gravity Cells. Science. 1963 Mar 1;139(3557):837–838. doi: 10.1126/science.139.3557.837. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES