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. 1992 Jan 15;281(Pt 2):369–375. doi: 10.1042/bj2810369

Coupling of D2 dopamine receptors to G-proteins in solubilized preparations of bovine caudate nucleus.

J A Chazot 1, P G Strange 1
PMCID: PMC1130693  PMID: 1736886

Abstract

1. The coupling of D2 dopamine receptors and G-proteins has been examined in cholate-solubilized preparations of bovine caudate nucleus. 2. No receptor-G-protein coupling could be detected in solubilized preparations obtained in 0.3% cholate, but if this preparation is diluted 5-fold, coupling is re-established. 3. The dilution process was examined, and it was shown that the change in ionic strength was an important factor in modulating the observed receptor-G-protein interaction. 4. Ionic strength was shown, however, not to be the primary determinant of receptor-G-protein coupling. This is likely to be the formation, upon dilution of the preparation, of vesicles in which receptor and G-protein reassociate. 5. The formation of vesicles upon dilution was examined by a variety of techniques, including thermal-stability studies, gel filtration, centrifugation and electron microscopy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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