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. 1976 May;30(5):735–739.

The differential effect of cyclic AMP on lymphocyte stimulation by T- or B-cell mitogens.

T L Vischer
PMCID: PMC1445042  PMID: 179940

Abstract

Dibutyryl-cyclic adenosine 3'5'-monophosphate (cAMP) added to mouse spleen cell cultures under serum free conditions inhibited the stimulation of [3H]thymidine incorporation induced by T-cell mitogens much more than the one induced by B-cell mitogens. The inhibition was most impressive with phytohaemagglutinin, followed by concanavalin A, pokeweed mitogen and the specific antigen Keyhole limpet haemocyanin (KLH). The response to bacterial lipopolysaccharide (LPS) was clearly less susceptible to suppression and the effect on the stimulation induced by trypsin and suramin, both B-cell mitogens, was marginal. Similar results were obtained by addition of isoproterenol or therophyllin to the cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Sjöberg O., Möller G. Mitogens as probes for immunocyte activation and cellular cooperation. Transplant Rev. 1972;11:131–177. doi: 10.1111/j.1600-065x.1972.tb00048.x. [DOI] [PubMed] [Google Scholar]
  2. Bach M. A. Differences in Cyclic AMP Changes after Stimulation by Prostaglandins and Isoproterenol in Lymphocyte Subpopulations. J Clin Invest. 1975 May;55(5):1074–1081. doi: 10.1172/JCI108008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Diamantstein T., Ulmer A. The antagonistic action of cyclic GMP and cyclic AMP on proliferation of B and T lymphocytes. Immunology. 1975 Jan;28(1):113–119. [PMC free article] [PubMed] [Google Scholar]
  4. Epstein L. B., Cline M. J., Merigan T. C. The interaction of human macrophages and lymphocytes in the phytohemagglutinin-stimulated production of interferon. J Clin Invest. 1971 Apr;50(4):744–753. doi: 10.1172/JCI106545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gallily R., Garvey J. S. Primary stimulation of rats and mice with hemocyanin in soluton and adsorbed on bentonite. J Immunol. 1968 Nov;101(5):924–929. [PubMed] [Google Scholar]
  6. Gery I., Waksman B. H. Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s). J Exp Med. 1972 Jul 1;136(1):143–155. doi: 10.1084/jem.136.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Greaves M., Janossy G. Elicitation of selective T and B lymphocyte responses by cell surface binding ligands. Transplant Rev. 1972;11:87–130. doi: 10.1111/j.1600-065x.1972.tb00047.x. [DOI] [PubMed] [Google Scholar]
  8. Hadden J. W., Hadden E. M., Haddox M. K., Goldberg N. D. Guanosine 3':5'-cyclic monophosphate: a possible intracellular mediator of mitogenic influences in lymphocytes. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3024–3027. doi: 10.1073/pnas.69.10.3024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heilman D. H., Gambrill M. R. Requirement of the macrophage or its products for the transformation of cortisol-resistant human lymphocytes by phytohemagglutinin. Immunol Commun. 1974;3(2):97–107. doi: 10.3109/08820137409055749. [DOI] [PubMed] [Google Scholar]
  10. Lichtenstein L. M., Henney C. S., Bourne H. R., Greenough W. B., 3rd Effects of cholera toxin on in vitro models of immediate and delayed hypersensitivity. Further evidence for the role of cyclic adenosine 3',5'-monophosphate. J Clin Invest. 1973 Mar;52(3):691–697. doi: 10.1172/JCI107230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Quagliata F., Lawrence V. J., Phillips-Quagliata J. M. Prostaglandin E 1 as a regulator of lymphocyte function. Selective action on B lymphocytes and synergy with procarbazine in depression of immune responses. Cell Immunol. 1973 Mar;6(3):457–465. doi: 10.1016/0008-8749(73)90044-0. [DOI] [PubMed] [Google Scholar]
  12. Takiguchi T., Adler W. H., Smith R. T. Cellular recognition in vitro by mouse lymphocytes. Effects of neonatal thymectomy and thymus graft restoration on alloantigen and PHA stimulation of whole and gradient-separated subpopulations of spleen cells. J Exp Med. 1971 Jan 1;133(1):63–80. doi: 10.1084/jem.133.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vischer T. L. Immunoglobulin-like surface molecules and theta antigen during the specific and non-specific stimulation of mouse spleen cells in vitro. Clin Exp Immunol. 1972 Aug;11(4):523–534. [PMC free article] [PubMed] [Google Scholar]
  14. Vischer T. L., LoSpalluto J. J. The differential effect of cholera toxin on the lymphocyte stimulation induced by various mitogens. Immunology. 1975 Aug;29(2):275–282. [PMC free article] [PubMed] [Google Scholar]
  15. Vischer T. L. Stimulation of mouse B lymphocytes by trypsin. J Immunol. 1974 Jul;113(1):58–62. [PubMed] [Google Scholar]
  16. Wahl S. M., Wilton J. M., Rosenstreich D. L., Oppenheim J. J. The role of macrophages in the production of lymphokines by T and B lymphocytes. J Immunol. 1975 Apr;114(4):1296–1301. [PubMed] [Google Scholar]
  17. Weissmann G., Dukor P., Zurier R. B. Effect of cyclic AMP on release of lysosomal enzymes from phagocytes. Nat New Biol. 1971 Jun 2;231(22):131–135. doi: 10.1038/newbio231131a0. [DOI] [PubMed] [Google Scholar]

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