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. 1991 Aug;59(8):2836–2838. doi: 10.1128/iai.59.8.2836-2838.1991

Antibody response of humans to the circumsporozoite protein of Plasmodium vivax.

E D Franke 1, C M Lucas 1, E San Roman 1
PMCID: PMC258094  PMID: 1855998

Abstract

We studied the interaction of sera from residents of an area in northern Peru where vivax malaria is endemic with four recombinant DNA-derived circumsporozoite (CS) proteins of Plasmodium vivax. The antigens used in the enzyme-linked immunosorbent assay included one Escherichia coli-produced and three Saccharomyces cerevisiae-produced recombinant proteins. Three of the proteins (NS1(81)V20, Vivax-1, and Vivax-2) contain the entire central repeat region of the P. vivax CS protein, and one protein (Vivax-3) contains only two repeat sequences. Vivax-1, Vivax-2, and Vivax-3 contain different lengths of sequences flanking the repeats. A higher percentage of the sera had antibodies to Vivax-2 and Vivax-3, the two proteins containing the longest nonrepeat sequences, than to NS1(81)V20 or Vivax-1. Children less than 5 years of age did not have immunoglobulin G antibodies to NS1(81)V20; however, they had antibodies to Vivax-1, Vivax-2, and Vivax-3. The finding that individuals living in a malaria-endemic area produce antibodies to peptides containing nonrepeat regions of the CS protein emphasizes the need to characterize the immune response to these regions in naturally exposed and experimentally immunized humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barr P. J., Gibson H. L., Enea V., Arnot D. E., Hollingdale M. R., Nussenzweig V. Expression in yeast of a Plasmodium vivax antigen of potential use in a human malaria vaccine. J Exp Med. 1987 Apr 1;165(4):1160–1171. doi: 10.1084/jem.165.4.1160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. George F. W., 4th, Law J. L., Rich K. A., Martin W. J. Identification of a T-cell epitope on the circumsporozoite protein of Plasmodium vivax. Infect Immun. 1990 Feb;58(2):575–578. doi: 10.1128/iai.58.2.575-578.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. McCutchan T. F., Lal A. A., de la Cruz V. F., Miller L. H., Maloy W. L., Charoenvit Y., Beaudoin R. L., Guerry P., Wistar R., Jr, Hoffman S. L. Sequence of the immunodominant epitope for the surface protein on sporozoites of Plasmodium vivax. Science. 1985 Dec 20;230(4732):1381–1383. doi: 10.1126/science.2416057. [DOI] [PubMed] [Google Scholar]
  4. Nardin E. H., Barr P. J., Heimer E., Etlinger H. M. Genetic restriction of the murine humoral response to a recombinant Plasmodium vivax circumsporozoite protein. Eur J Immunol. 1988 Jul;18(7):1119–1122. doi: 10.1002/eji.1830180722. [DOI] [PubMed] [Google Scholar]
  5. Rich K. A., George F. W., 4th, Law J. L., Martin W. J. Cell-adhesive motif in region II of malarial circumsporozoite protein. Science. 1990 Sep 28;249(4976):1574–1577. doi: 10.1126/science.2120774. [DOI] [PubMed] [Google Scholar]
  6. Romero P., Heimer E. P., Herrera S., Felix A. M., Nussenzweig R. S., Zavala F. Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax. J Immunol. 1987 Sep 1;139(5):1679–1682. [PubMed] [Google Scholar]
  7. Rosenberg R., Wirtz R. A., Lanar D. E., Sattabongkot J., Hall T., Waters A. P., Prasittisuk C. Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax. Science. 1989 Sep 1;245(4921):973–976. doi: 10.1126/science.2672336. [DOI] [PubMed] [Google Scholar]
  8. Sharma S., Gwadz R. W., Schlesinger D. H., Godson G. N. Immunogenicity of the repetitive and nonrepetitive peptide regions of the divergent CS protein of Plasmodium knowlesi. J Immunol. 1986 Jul 1;137(1):357–361. [PubMed] [Google Scholar]
  9. Vergara U., Gwadz R., Schlesinger D., Nussenzweig V., Ferreira A. Multiple non-repeated epitopes on the circumsporozoite protein of Plasmodium knowlesi. Mol Biochem Parasitol. 1985 Mar;14(3):283–292. doi: 10.1016/0166-6851(85)90056-8. [DOI] [PubMed] [Google Scholar]
  10. Wirtz R. A., Duncan J. F., Njelesani E. K., Schneider I., Brown A. E., Oster C. N., Were J. B., Webster H. K. ELISA method for detecting Plasmodium falciparum circumsporozoite antibody. Bull World Health Organ. 1989;67(5):535–542. [PMC free article] [PubMed] [Google Scholar]

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