Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1991 Sep;85(3):436–440. doi: 10.1111/j.1365-2249.1991.tb05745.x

Increased luminol-enhanced chemiluminescence of blood monocytes and granulocytes in Hodgkin's disease.

O Tullgren 1, R Giscombe 1, G Holm 1, B Johansson 1, H Mellstedt 1, M Björkholm 1
PMCID: PMC1535611  PMID: 1893624

Abstract

The oxidative metabolic burst of blood monocytes and polymorphonuclear leukocytes (PMN) from 22 untreated patients with Hodgkin's disease (HD) and 18 healthy subjects were studied. Monocytes and PMN were enriched by density centrifugation and in vitro activated by zymosan. The oxidative metabolism was measured by luminol-enhanced chemiluminescence (CL). The CL of the patients' monocytes and PMN was higher than that of controls (P less than 0.01 and P less than 0.05, respectively). Patients with stage II-IV HD showed an increased blood monocyte CL as compared with stage I patients (P less than 0.05). Furthermore, patients with lymphocytic depletion or mixed cellularity subtype demonstrated an increased CL of PMN as compared with the remainder. Enhanced CL of phagocytes has been observed in chronic inflammatory disease and can be induced by various serum factors such as monokines and immune complexes. The present study demonstrates an increased CL of blood-borne phagocytic cells in untreated HD. Furthermore, CL of blood monocytes and PMN correlated to tumour burden and histologic subtype, respectively.

Full text

PDF
436

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. C., Stjernholm R. L., Steele R. H. Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochem Biophys Res Commun. 1972 May 26;47(4):679–684. doi: 10.1016/0006-291x(72)90545-1. [DOI] [PubMed] [Google Scholar]
  2. Anton P. A., Targan S. R., Vigna S. R., Durham M., Schwabe A. D., Shanahan F. Enhanced neutrophil chemiluminescence in familial Mediterranean fever. J Clin Immunol. 1988 Mar;8(2):148–156. doi: 10.1007/BF00917903. [DOI] [PubMed] [Google Scholar]
  3. Asman B., Engström P. E., Olsson T., Bergström K. Increased luminol enhanced chemiluminescence from peripheral granulocytes in juvenile periodontitis. Scand J Dent Res. 1984 Jun;92(3):218–223. doi: 10.1111/j.1600-0722.1984.tb00882.x. [DOI] [PubMed] [Google Scholar]
  4. Barbour A. G., Allred C. D., Solberg C. O., Hill H. R. Chemiluminescence by polymorphonuclear leukocytes from patients with active bacterial infection. J Infect Dis. 1980 Jan;141(1):14–26. doi: 10.1093/infdis/141.1.14. [DOI] [PubMed] [Google Scholar]
  5. Berge L. N., Ostensen M., Revhaug A. Phagocytic cell activity in pre-eclampsia. Acta Obstet Gynecol Scand. 1988;67(6):499–504. doi: 10.3109/00016348809029860. [DOI] [PubMed] [Google Scholar]
  6. Björkholm M. Immunodeficiency in Hodgkin's disease and its relation to prognosis. Scand J Haematol Suppl. 1978;33:1–74. [PubMed] [Google Scholar]
  7. Björkholm M., Wedelin C., Holm G., Ogenstad S., Johansson B., Mellstedt H. Immune status of untreated patients with Hodgkin's disease and prognosis. Cancer Treat Rep. 1982 Apr;66(4):701–709. [PubMed] [Google Scholar]
  8. Carbone P. P., Kaplan H. S., Musshoff K., Smithers D. W., Tubiana M. Report of the Committee on Hodgkin's Disease Staging Classification. Cancer Res. 1971 Nov;31(11):1860–1861. [PubMed] [Google Scholar]
  9. Case D. C., Hansen J. A., Corrales E., Young C. W., Dupont B., Pinsky C. M., Good R. A. Comparison of multiple in vivo and in vitro parameters in untreated patients with Hodgkin's disease. Cancer. 1976 Oct;38(4):1807–1815. doi: 10.1002/1097-0142(197610)38:4<1807::aid-cncr2820380458>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  10. Corberand J., Benchekroun S., Nguyen F., Laharrague P., Pris J. Polymorphonuclear functions in Hodgkin's disease patients at diagnosis, in remission, and in relapse. Cancer Res. 1982 Apr;42(4):1595–1599. [PubMed] [Google Scholar]
  11. Estevez M. E., Ballart I. J., de Macedo M. P., Magnasco H., Nicastro M. A., Sen L. Dysfunction of monocytes in Hodgkin's disease by excessive production of PGE-2 in long-term remission patients. Cancer. 1988 Nov 15;62(10):2128–2133. doi: 10.1002/1097-0142(19881115)62:10<2128::aid-cncr2820621011>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  12. Estevez M. E., Sen L., Bachmann A. E., Pavlovsky A. Defective function of peripheral blood monocytes in patients with Hodgkin's and non-Hodgkin's lymphomas. Cancer. 1980 Jul 15;46(2):299–302. doi: 10.1002/1097-0142(19800715)46:2<299::aid-cncr2820460214>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  13. Gandossini M., Souhami R. L., Babbage J., Addison I. E., Johnson A. L., Berenbaum M. C. Neutrophil function during chemotherapy for Hodgkin's disease. Br J Cancer. 1981 Dec;44(6):863–871. doi: 10.1038/bjc.1981.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Goffinet D. R., Glatstein E. J., Merigan T. C. Herpes Zoster-Varicella infections and lymphoma. Ann Intern Med. 1972 Feb;76(2):235–240. doi: 10.7326/0003-4819-76-2-235. [DOI] [PubMed] [Google Scholar]
  15. Hancock B. W., Bruce L., Richmond J. Neutrophil function in lymphoreticular malignancy. Br J Cancer. 1976 May;33(5):496–500. doi: 10.1038/bjc.1976.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hersh E. M., Oppenheim J. J. Impaired in vitro lymphocyte transformation in Hodgkin's disease. N Engl J Med. 1965 Nov 4;273(19):1006–1012. doi: 10.1056/NEJM196511042731903. [DOI] [PubMed] [Google Scholar]
  17. Holm G., Mellstedt H., Björkholm M., Johansson B., Killander D., Sundblad R., Söderberg G. Lymphocyte abnormalities in untreated patients with Hodgkin's disease. Cancer. 1976 Feb;37(2):751–762. doi: 10.1002/1097-0142(197602)37:2<751::aid-cncr2820370223>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  18. Holm G., Perlmann P., Johansson B. Impaired phytohaemagglutinin-induced cytotoxicity in vitro of lymphocytes from patients with Hodgkin's disease or chronic lymphatic leukaemia. Clin Exp Immunol. 1967 May;2(3):351–360. [PMC free article] [PubMed] [Google Scholar]
  19. Leb L., Merritt J. A. Decreased monocyte function in patients with Hodgkin's disease. Cancer. 1978 May;41(5):1794–1803. doi: 10.1002/1097-0142(197805)41:5<1794::aid-cncr2820410522>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  20. Meuret G., Schmitt E., Tseleni S., Widmer M. Monocyte production in Hodgkin's disease and non-Hodgkin's lymphoma. Blut. 1978 Oct 13;37(4):193–200. doi: 10.1007/BF00996720. [DOI] [PubMed] [Google Scholar]
  21. Notter D. T., Grossman P. L., Rosenberg S. A., Remington J. S. Infections in patients with Hodgkin's disease: a clinical study of 300 consecutive adult patients. Rev Infect Dis. 1980 Sep-Oct;2(5):761–800. doi: 10.1093/clinids/2.5.761. [DOI] [PubMed] [Google Scholar]
  22. Passwell J., Levanon M., Davidsohn J., Ramot B. Monocyte PGE2 secretion in Hodgkin's disease and its relation to decreased cellular immunity. Clin Exp Immunol. 1983 Jan;51(1):61–68. [PMC free article] [PubMed] [Google Scholar]
  23. Perez-Soler R., Lopez-Berestein G., Cabanillas F., McLaughlin P., Hersh E. M. Superoxide anion (O-2) production by peripheral blood monocytes in Hodgkin's disease and malignant lymphoma. J Clin Oncol. 1985 May;3(5):641–645. doi: 10.1200/JCO.1985.3.5.641. [DOI] [PubMed] [Google Scholar]
  24. Roberts R. L., Stiehm E. R. Increased phagocytic cell chemiluminescence in patients with cystic fibrosis. Am J Dis Child. 1989 Aug;143(8):944–950. doi: 10.1001/archpedi.1989.02150200104027. [DOI] [PubMed] [Google Scholar]
  25. Romagnani S., Del Prete G. F., Maggi E., Bellesi G., Biti G., Rossi Ferrini P. L., Ricci M. Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease. J Clin Invest. 1983 May;71(5):1375–1382. doi: 10.1172/JCI110890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ruskin J., Remington J. S. Pneumocystis carinii infection in the immunosuppressed host. Antimicrob Agents Chemother (Bethesda) 1967;7:70–76. [PubMed] [Google Scholar]
  27. Samoszuk M., Nansen L. Detection of interleukin-5 messenger RNA in Reed-Sternberg cells of Hodgkin's disease with eosinophilia. Blood. 1990 Jan 1;75(1):13–16. [PubMed] [Google Scholar]
  28. Schopf R. E., Mattar J., Meyenburg W., Scheiner O., Hammann K. P., Lemmel E. M. Measurement of the respiratory burst in human monocytes and polymorphonuclear leukocytes by nitro blue tetrazolium reduction and chemiluminescence. J Immunol Methods. 1984 Feb 24;67(1):109–117. doi: 10.1016/0022-1759(84)90090-5. [DOI] [PubMed] [Google Scholar]
  29. Sheagren J. N., Block J. B., Wolff S. M. Reticuloendothelial system phagocytic function in patients with Hodgkin's disease. J Clin Invest. 1967 May;46(5):855–862. doi: 10.1172/JCI105585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Simpson J. F., Leddy J. P., Hare J. D. Listeriosis complicating lymphoma. Report of four cases and interpretive review of pathogenetic factors. Am J Med. 1967 Jul;43(1):39–49. doi: 10.1016/0002-9343(67)90147-7. [DOI] [PubMed] [Google Scholar]
  31. Smit J. W., van der Giessen M., Halie M. R. Fc gamma receptors on lymphocytes from normal donors and patients with lymphoproliferative diseases. Influence of incubation conditions. Acta Haematol. 1983;70(2):108–118. doi: 10.1159/000206703. [DOI] [PubMed] [Google Scholar]
  32. Steigbigel R. T., Lambert L. H., Jr, Remington J. S. Polymorphonuclear leukocyte, monocyte, and macrophage bactericidal function in patients with Hodgkin's disease. J Lab Clin Med. 1976 Jul;88(1):54–62. [PubMed] [Google Scholar]
  33. Trulson A., Nilsson S., Venge P. Lucigenin-enhanced chemiluminescence in blood is increased in cancer. Am J Clin Pathol. 1989 Apr;91(4):441–445. doi: 10.1093/ajcp/91.4.441. [DOI] [PubMed] [Google Scholar]
  34. Vietzke W. M., Gelderman A. H., Grimley P. M., Valsamis M. P. Toxoplasmosis complicating malignancy. Experience at the National Cancer Institute. Cancer. 1968 May;21(5):816–827. doi: 10.1002/1097-0142(196805)21:5<816::aid-cncr2820210506>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  35. Wedelin C., Björkholm M., Johansson B., Mellstedt H. Clinical and laboratory findings in untreated patients with Hodgkin's disease with special reference to age. Med Oncol Tumor Pharmacother. 1984;1(1):33–41. doi: 10.1007/BF02935323. [DOI] [PubMed] [Google Scholar]
  36. Yuo A., Kitagawa S., Ohsaka A., Ohta M., Miyazono K., Okabe T., Urabe A., Saito M., Takaku F. Recombinant human granulocyte colony-stimulating factor as an activator of human granulocytes: potentiation of responses triggered by receptor-mediated agonists and stimulation of C3bi receptor expression and adherence. Blood. 1989 Nov 1;74(6):2144–2149. [PubMed] [Google Scholar]
  37. Zeller J. M., Caliendo J., Lint T. F., Nelson D. J. Changes in respiratory burst activity during human monocyte differentiation in suspension culture. Inflammation. 1988 Dec;12(6):585–595. doi: 10.1007/BF00914320. [DOI] [PubMed] [Google Scholar]
  38. de Mulder P. H., Mier P. D., de Pauw B. E., van Rennes H., Janssen J. T., Wagener D. J., Haanen C. Altered intracellular enzyme activity of monocytes and lymphocytes in Hodgkin's disease. Eur J Cancer Clin Oncol. 1983 Mar;19(3):333–337. doi: 10.1016/0277-5379(83)90130-x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES