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. 2003 May;42(1):13–20. doi: 10.1023/A:1026157515032

Production of interferon-β by NB1-RGB cells cultured on peptide-lipid membranes

Mariko Hara 1, Yasunari Takanashi 1, Nobuya Tuzuki 1, Hiroko Kawakami 2, Kazunori Toma 2, Akon Higuchi 1
PMCID: PMC3449508  PMID: 19002924

Abstract

Cell growth and production of interferon-β (IFN-β) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and arginine-glutamic acid-aspartic acid-valine [Arg-Glu-Asp-Val] (REDV) peptides. Cell density was found to be approximately the same on various peptide-lipid membranes, whereas production of IFN-β depended significantly on the peptide-lipid membranes on which NB1-RGB cells were cultured. The highest production of IFN-β was observed for NB1-RGB cells on REDV-lipid membranes prepared by a casting method (REDV-cast membranes) after 24 hr of cultivation. Specific binding between REDV of REDV-cast membranes and the receptor on the NB1-RGB cells may have caused the specific cell response for the production of IFN-β.

Keywords: Cell culture, Fibroblast cells, Interferon-β, Langmuir-Blodgett membrane, Peptide-lipid

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