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. 1999 Jan;29(1):65–70. doi: 10.1023/A:1007993615489

Preparation of monoclonal antibody against crocin and its characterization

Lijiang Xuan 1, Hiroyuki Tanaka 1, Yaming Xu 2, Yukihiro Shoyama 1,✉
PMCID: PMC3449468  PMID: 19003338

Abstract

Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG2a and IgG2b possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.

Keywords: crocetin glycosides, crocin, Crocus sativus, ELISA, MALDI-TOF-MS, monoclonal antibody

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