Abstract
Sera from patients with yersiniosis, shown to contain antibodies to Yersinia enterocolitica O9; and sera from patients with haemolytic uraemic syndrome (HUS) caused by Escherichia coli O157, were used to investigate serological cross-reactions between Y. enterocolitica O9 and E. coli O157. Lipopolysaccharide (LPS) was isolated from strains of Y. enterocolitica O9 and E. coli O157 and reacted with sera by immunoblotting and ELISA. Sera from patients with HUS contained antibodies to the LPS of E. coli O157 only; 80% of sera from patients with yersiniosis contained antibodies to the LPS of Y. enterocolitica O9 and E. coli O157. This one-way cross-reaction was also detected using hyperimmune rabbit antisera.
Full text
PDF







Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caroff M., Bundle D. R., Perry M. B. Structure of the O-chain of the phenol-phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9. Eur J Biochem. 1984 Feb 15;139(1):195–200. doi: 10.1111/j.1432-1033.1984.tb07994.x. [DOI] [PubMed] [Google Scholar]
- Chart H., Scotland S. M., Rowe B. Bacterial antigenic cross-reactions and haemolytic uraemic syndrome. Lancet. 1988 Aug 27;2(8609):510–511. doi: 10.1016/s0140-6736(88)90156-0. [DOI] [PubMed] [Google Scholar]
- Chart H., Scotland S. M., Rowe B. Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. J Clin Microbiol. 1989 Feb;27(2):285–290. doi: 10.1128/jcm.27.2.285-290.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chart H., Scotland S. M., Smith H. R., Rowe B. Antibodies to Escherichia coli O157 in patients with haemorrhagic colitis and haemolytic uraemic syndrome. J Clin Pathol. 1989 Sep;42(9):973–976. doi: 10.1136/jcp.42.9.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Corbell M. J. The serological relationship between Brucella spp., Yersinia enterocolitica serotype IX and Salmonella serotypes of Kauffmann-White group N. J Hyg (Lond) 1975 Aug;75(1):151–171. doi: 10.1017/s0022172400047173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilmour A., Walker S. J. Isolation and identification of Yersinia enterocolitica and the Yersinia enterocolitica-like bacteria. Soc Appl Bacteriol Symp Ser. 1988;17:213S–236S. [PubMed] [Google Scholar]
- Griffiths E., Stevenson P., Thorpe R., Chart H. Naturally occurring antibodies in human sera that react with the iron-regulated outer membrane proteins of Escherichia coli. Infect Immun. 1985 Mar;47(3):808–813. doi: 10.1128/iai.47.3.808-813.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Levin M., Walters M. D., Barratt T. M. Hemolytic uremic syndrome. Adv Pediatr Infect Dis. 1989;4:51–81. [PubMed] [Google Scholar]
- Lindberg A. A., Haeggman S., Karlson K., Carlsson H. E., Mair N. S. Enzyme immunoassay of the antibody response to Brucella and Yersinia enterocolitica 09 infections in humans. J Hyg (Lond) 1982 Apr;88(2):295–307. doi: 10.1017/s0022172400070157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Notenboom R. H., Borczyk A., Karmali M. A., Duncan L. M. Clinical relevance of a serological cross-reaction between Escherichia coli O157 and Brucella abortus. Lancet. 1987 Sep 26;2(8561):745–745. doi: 10.1016/s0140-6736(87)91106-8. [DOI] [PubMed] [Google Scholar]
- Perry M. B., MacLean L., Griffith D. W. Structure of the O-chain polysaccharide of the phenol-phase soluble lipopolysaccharide of Escherichia coli 0:157:H7. Biochem Cell Biol. 1986 Jan;64(1):21–28. doi: 10.1139/o86-004. [DOI] [PubMed] [Google Scholar]
- Sandulache R., Marx A. Immunochemical studies on a Yersinia enterocolitica O:9 lipopolysaccharide cross-reacting with Brucella abortus and Vibrio cholerae extracts. Ann Microbiol (Paris) 1978 Oct;129 B(3):425–435. [PubMed] [Google Scholar]
- Scotland S. M., Rowe B., Smith H. R., Willshaw G. A., Gross R. J. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol. 1988 Apr;25(4):237–243. doi: 10.1099/00222615-25-4-237. [DOI] [PubMed] [Google Scholar]
- Stuart F. A., Corbel M. J. Identification of a serological cross-reaction between Brucella abortus and Escherichia coli 0:157. Vet Rec. 1982 Feb 27;110(9):202–203. doi: 10.1136/vr.110.9.202-a. [DOI] [PubMed] [Google Scholar]
- Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
- Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]