Skip to main content
NCBI home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1977 Apr;41(2):160–165.

Neutralizing activity in the gastrointestinal contents of piglets vaccinated with an ethylenimine-inactivated porcine enterovirus.

D G Hazlett, J B Derbyshire
PMCID: PMC1277713  PMID: 193624

Abstract

The T80 strain of porcine enterovirus was rapidly and completely inactiviated by ethylenimine in a reaction which appeared to follow first order kinetics. The virus was effectively concentrated 35- to 88-fold, with recovery rates of 23 t0 53%, by adsorption to the polyelectrolyte PE60. Multiple doses of adjuvanted, PE60-concentrated, ethylenimine-inactivated T80 virus given by both the oral and subcutaneous routes induced the appearance of significant levels of virus neutralizing activity in the gastrointestinal tract of piglets vaccinated at four weeks of age. This activity, found predominantly in large intestine, was present 14 days after administration of the first dose of vaccine and significant levels of activity were still detectable six weeks later. Titres of serum virus neutralizing activity were higher and more persistent than in piglets which received live or formaldehyde-inactivated T80 virus by the oral or intramuscular routes.

Full text

PDF
160

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. C., Masters R. C., Mowat G. N. Immune response of pigs to inactivated foot-and-mouth disease vaccines. Response to DEAE-dextran and saponin adjuvanted vaccines. Res Vet Sci. 1971 Jul;12(4):351–357. [PubMed] [Google Scholar]
  2. Anderson E. C., Masters R. C., Mowat G. N. Immune response of pigs to inactivated foot-and-mouth disease vaccines. Response to emulsion vaccines. Res Vet Sci. 1971 Jul;12(4):342–350. [PubMed] [Google Scholar]
  3. BROWN F., CARTWRIGHT B., STEWART D. L. The effect of various inactivating agents on the viral and ribonucleic acid infectivities of foot-and-mouth disease virus and on its attachment to susceptible cells. J Gen Microbiol. 1963 May;31:179–186. doi: 10.1099/00221287-31-2-179. [DOI] [PubMed] [Google Scholar]
  4. Bahnemann H. G. The inactivation of foot-and-mouth disease virus by ethylenimine and propylenimine. Zentralbl Veterinarmed B. 1972 Jul;20(5):356–360. doi: 10.1111/j.1439-0450.1973.tb01136.x. [DOI] [PubMed] [Google Scholar]
  5. CLIVER D. O. FACTORS IN THE MEMBRANE FILTRATION OF ENTEROVIRUSES. Appl Microbiol. 1965 May;13:417–431. doi: 10.1128/am.13.3.417-425.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cunliffe H. R. Inactivation of foot-and-mouth disease virus with ethylenimine. Appl Microbiol. 1973 Nov;26(5):747–750. doi: 10.1128/am.26.5.747-750.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Derbyshire J. B. Enterovirus neutralizing activity in the gastrointestinal tract of piglets. Can J Comp Med. 1974 Oct;38(4):425–429. [PMC free article] [PubMed] [Google Scholar]
  8. Fedson D. S., Fulk R. V., Huber M. A., Reisberg M. A., Kasel J. A. Anti-neuraminidase antibody response in serum and nasal secretions following intranasal or subcutaneous inactivated A2-Hong Kong-68 influenza virus vaccine. J Immunol. 1971 Sep;107(3):730–737. [PubMed] [Google Scholar]
  9. Freestone D. S., Hamilton-Smith S., Schild G. C., Buckland R., Chinn S., Tyrrell D. A. Antibody responses and resistance to challenge in volunteers vaccinated with live attenuated, detergent split and oil adjuvant A2-Hong Kong-68 (H 3 N 2 ) influenza vaccines. A report to the Medical Research Council Committee on Influenza and other Respiratory Virus Vaccines. J Hyg (Lond) 1972 Sep;70(3):531–543. doi: 10.1017/s0022172400063117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fulk R. V., Fedson D. S., Huber M. A., Fitzpatrick J. R., Howar B. F., Kasel J. A. Antibody responses in children and elderly persons following local or parenteral administration of an inactivated influenza virus vaccine, A2-Hong Kong-68 variant. J Immunol. 1969 Apr;102(4):1102–1105. [PubMed] [Google Scholar]
  11. Graves J. H., Arlinghaus R. B. Acetylethyleneimine in the preparation of inactivated food-and-mouth disease vaccines. Proc Annu Meet U S Anim Health Assoc. 1967;71:396–403. [PubMed] [Google Scholar]
  12. Hazlett D. T., Derbyshire J. B. Neutralizing activity in the gastrointestinal contents of piglets vaccinated with a live or formaldehyde-inactivated porcine enterovirus. Can J Comp Med. 1976 Oct;40(4):370–379. [PMC free article] [PubMed] [Google Scholar]
  13. Herbert W. J. Multiple emulsions. A new form of mineral-oil antigen adjuvant. Lancet. 1965 Oct 16;2(7416):771–771. doi: 10.1016/s0140-6736(65)90816-0. [DOI] [PubMed] [Google Scholar]
  14. Hilleman M. R., Woodhour A., Friedman A., Weibel R. E., Stokes J., Jr The clinical application of adjuvant 65. Ann Allergy. 1972 Mar;30(3):152–158. [PubMed] [Google Scholar]
  15. Husband A. J., Lascelles A. K. The origin of antibody in intestinal secretion of sheep. Aust J Exp Biol Med Sci. 1974 Oct;52(5):791–799. doi: 10.1038/icb.1974.78. [DOI] [PubMed] [Google Scholar]
  16. Kawakami K., Tatsumi H., Tatsumi M., Kono R. Studies on poliovirus coproantibody. I. Neutralizing antibodies in feces of children following Sabin oral poliovirus vaccination. Am J Epidemiol. 1966 Jan;83(1):1–13. doi: 10.1093/oxfordjournals.aje.a120557. [DOI] [PubMed] [Google Scholar]
  17. McKercher P. D., Gailiunas P. Response of swine to inactivated foot-and-mouth disease vaccine. Duration of immunity and local tissue reaction. Arch Gesamte Virusforsch. 1969;28(2):165–176. doi: 10.1007/BF01249381. [DOI] [PubMed] [Google Scholar]
  18. McKercher P. D., Giordano A. R. Foot-and-mouth disease in swine. I. The immune response of swine of chemically-treated and non-treated foot-and-mouth disease virus. Arch Gesamte Virusforsch. 1967;20(1):39–53. doi: 10.1007/BF01245768. [DOI] [PubMed] [Google Scholar]
  19. Mowat G. N., Prince M. J., Spier R. E., Staple R. F. Preliminary studies on the development of a swine vesicular disease vaccine. Arch Gesamte Virusforsch. 1974;44(4):350–360. doi: 10.1007/BF01251016. [DOI] [PubMed] [Google Scholar]
  20. Wallis C., Melnick J. L., Fields J. E. Concentration and purification of viruses by adsorption to and elution from insoluble polyelectrolytes. Appl Microbiol. 1971 Apr;21(4):703–709. doi: 10.1128/am.21.4.703-709.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES