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. 1991 Mar;44(3):177–181. doi: 10.1136/jcp.44.3.177

In situ hybridisation in perspective.

A Warford 1, I Lauder 1
PMCID: PMC496932  PMID: 2013617

Abstract

In the introduction to this review two questions were posed: is the technology associated with ISH ready for general use, and will the method become an important investigative tool? With the exception of the demonstration of some single and low copy sequences, non-radioactive ISH is now sufficiently developed and simplified to make it a routine technique. It is also clear that ISH will continue to have an important research role. In diagnostic pathology the technique is already providing valuable information and the present decade should see the development of many more diagnostic applications.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akhtar N., Ruprai A., Pringle J. H., Lauder I., Durrant S. T. In situ hybridization detection of light chain mRNA in routine bone marrow trephines from patients with suspected myeloma. Br J Haematol. 1989 Nov;73(3):296–301. doi: 10.1111/j.1365-2141.1989.tb07743.x. [DOI] [PubMed] [Google Scholar]
  2. Allan G. M., Todd D., Smyth J. A., Mackie D. P., Burns J., McNulty M. S. In situ hybridization: an optimised detection protocol for a biotinylated DNA probe renders it more sensitive than a comparable 35S-labelled probe. J Virol Methods. 1989 Apr-May;24(1-2):181–190. doi: 10.1016/0166-0934(89)90020-7. [DOI] [PubMed] [Google Scholar]
  3. Angerer L. M., Chambers S. A., Yang Q., Venkatesan M., Angerer R. C., Simpson R. T. Expression of a collagen gene in mesenchyme lineages of the Strongylocentrotus purpuratus embryo. Genes Dev. 1988 Feb;2(2):239–246. doi: 10.1101/gad.2.2.239. [DOI] [PubMed] [Google Scholar]
  4. Banerjee D., Pettit S. Endogenous avidin-binding activity in human lymphoid tissue. J Clin Pathol. 1984 Feb;37(2):223–225. doi: 10.1136/jcp.37.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barr B. B., Benton E. C., McLaren K., Bunney M. H., Smith I. W., Blessing K., Hunter J. A. Human papilloma virus infection and skin cancer in renal allograft recipients. Lancet. 1989 Jan 21;1(8630):124–129. doi: 10.1016/s0140-6736(89)91143-4. [DOI] [PubMed] [Google Scholar]
  6. Bashir R. M., Harris N. L., Hochberg F. H., Singer R. M. Detection of Epstein-Barr virus in CNS lymphomas by in-situ hybridization. Neurology. 1989 Jun;39(6):813–817. doi: 10.1212/wnl.39.6.813. [DOI] [PubMed] [Google Scholar]
  7. Beckmann A. M., Daling J. R., Sherman K. J., Maden C., Miller B. A., Coates R. J., Kiviat N. B., Myerson D., Weiss N. S., Hislop T. G. Human papillomavirus infection and anal cancer. Int J Cancer. 1989 Jun 15;43(6):1042–1049. doi: 10.1002/ijc.2910430615. [DOI] [PubMed] [Google Scholar]
  8. Bender H. G., Degen K. W., Beck L. Human papilloma virus findings in the perimeter of vulvo-vaginal malignancies. Eur J Gynaecol Oncol. 1988;9(4):287–290. [PubMed] [Google Scholar]
  9. Blum H. E., Stowring L., Figus A., Montgomery C. K., Haase A. T., Vyas G. N. Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization. Proc Natl Acad Sci U S A. 1983 Nov;80(21):6685–6688. doi: 10.1073/pnas.80.21.6685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Buongiorno-Nardelli M., Amaldi F. Autoradiographic detection of molecular hybrids between RNA and DNA in tissue sections. Nature. 1970 Mar 7;225(5236):946–948. doi: 10.1038/225946a0. [DOI] [PubMed] [Google Scholar]
  11. Burns J., Chan V. T., Jonasson J. A., Fleming K. A., Taylor S., McGee J. O. Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells. J Clin Pathol. 1985 Oct;38(10):1085–1092. doi: 10.1136/jcp.38.10.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Burns J., Graham A. K., Frank C., Fleming K. A., Evans M. F., McGee J. O. Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation. J Clin Pathol. 1987 Aug;40(8):858–864. doi: 10.1136/jcp.40.8.858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Burns J., Graham A. K., McGee J. O. Non-isotopic detection of in situ nucleic acid in cervix: an updated protocol. J Clin Pathol. 1988 Aug;41(8):897–899. doi: 10.1136/jcp.41.8.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Burns J., Redfern D. R., Esiri M. M., McGee J. O. Human and viral gene detection in routine paraffin embedded tissue by in situ hybridisation with biotinylated probes: viral localisation in herpes encephalitis. J Clin Pathol. 1986 Oct;39(10):1066–1073. doi: 10.1136/jcp.39.10.1066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Butler A. E., Colby T. V., Weiss L., Lombard C. Lymphoepithelioma-like carcinoma of the lung. Am J Surg Pathol. 1989 Aug;13(8):632–639. doi: 10.1097/00000478-198908000-00002. [DOI] [PubMed] [Google Scholar]
  16. Collins J. E., Jenkins D., McCance D. J. Detection of human papillomavirus DNA sequences by in situ DNA-DNA hybridisation in cervical intraepithelial neoplasia and invasive carcinoma: a retrospective study. J Clin Pathol. 1988 Mar;41(3):289–295. doi: 10.1136/jcp.41.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Cox K. H., DeLeon D. V., Angerer L. M., Angerer R. C. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol. 1984 Feb;101(2):485–502. doi: 10.1016/0012-1606(84)90162-3. [DOI] [PubMed] [Google Scholar]
  18. Davis M., Malcolm S., Rabbitts T. H. Chromosome translocation can occur on either side of the c-myc oncogene in Burkitt lymphoma cells. Nature. 1984 Mar 15;308(5956):286–288. doi: 10.1038/308286a0. [DOI] [PubMed] [Google Scholar]
  19. Emson P. C., Arai H., Agrawal S., Christodoulou C., Gait M. J. Nonradioactive methods of in situ hybridization: visualization of neuroendocrine mRNA. Methods Enzymol. 1989;168:753–761. doi: 10.1016/0076-6879(89)68056-1. [DOI] [PubMed] [Google Scholar]
  20. Eversole L. R., Stone C. E., Beckman A. M. Detection of EBV and HPV DNA sequences in oral "hairy" leukoplakia by in situ hybridization. J Med Virol. 1988 Nov;26(3):271–277. doi: 10.1002/jmv.1890260307. [DOI] [PubMed] [Google Scholar]
  21. Gall J. G., Pardue M. L. Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A. 1969 Jun;63(2):378–383. doi: 10.1073/pnas.63.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Gentilomi G., Musiani M., Zerbini M., Gallinella G., Gibellini D., La Placa M. A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes. J Immunol Methods. 1989 Dec 20;125(1-2):177–183. doi: 10.1016/0022-1759(89)90091-4. [DOI] [PubMed] [Google Scholar]
  23. Hankin R. C., Lloyd R. V. Detection of messenger RNA in routinely processed tissue sections with biotinylated oligonucleotide probes. Am J Clin Pathol. 1989 Aug;92(2):166–171. doi: 10.1093/ajcp/92.2.166. [DOI] [PubMed] [Google Scholar]
  24. Harabuchi Y., Yamanaka N., Kataura A., Imai S., Kinoshita T., Mizuno F., Osato T. Epstein-Barr virus in nasal T-cell lymphomas in patients with lethal midline granuloma. Lancet. 1990 Jan 20;335(8682):128–130. doi: 10.1016/0140-6736(90)90002-m. [DOI] [PubMed] [Google Scholar]
  25. Harrison P. R., Conkie D., Paul J., Jones K. Localisation of cellular globin messenger RNA by in situ hybridisation to complementary DNA. FEBS Lett. 1973 May 15;32(1):109–112. doi: 10.1016/0014-5793(73)80749-5. [DOI] [PubMed] [Google Scholar]
  26. Heath D. A., Senior P. V., Varley J. M., Beck F. Parathyroid-hormone-related protein in tumours associated with hypercalcaemia. Lancet. 1990 Jan 13;335(8681):66–69. doi: 10.1016/0140-6736(90)90537-f. [DOI] [PubMed] [Google Scholar]
  27. Herrington C. S., Burns J., Graham A. K., Bhatt B., McGee J. O. Interphase cytogenetics using biotin and digoxigenin labelled probes II: Simultaneous differential detection of human and papilloma virus nucleic acids in individual nuclei. J Clin Pathol. 1989 Jun;42(6):601–606. doi: 10.1136/jcp.42.6.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Herrington C. S., Burns J., Graham A. K., Evans M., McGee J. O. Interphase cytogenetics using biotin and digoxigenin labelled probes I: relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells. J Clin Pathol. 1989 Jun;42(6):592–600. doi: 10.1136/jcp.42.6.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Holland P. W., Harper S. J., McVey J. H., Hogan B. L. In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization. J Cell Biol. 1987 Jul;105(1):473–482. doi: 10.1083/jcb.105.1.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Hopman A. H., Ramaekers F. C., Raap A. K., Beck J. L., Devilee P., van der Ploeg M., Vooijs G. P. In situ hybridization as a tool to study numerical chromosome aberrations in solid bladder tumors. Histochemistry. 1988;89(4):307–316. doi: 10.1007/BF00500631. [DOI] [PubMed] [Google Scholar]
  31. Hopman A. H., Wiegant J., van Duijn P. A new hybridocytochemical method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands. II. Effects of variations in ligand structure on the in situ detection of mercurated probes. Histochemistry. 1986;84(2):179–185. doi: 10.1007/BF00499830. [DOI] [PubMed] [Google Scholar]
  32. Hudson P., Penschow J., Shine J., Ryan G., Niall H., Coghlan J. Hybridization histochemistry: use of recombinant DNA as a "homing probe" for tissue localization of specific mRNA populations. Endocrinology. 1981 Jan;108(1):353–356. doi: 10.1210/endo-108-1-353. [DOI] [PubMed] [Google Scholar]
  33. Hutchinson R. M., Pringle J. H., Potter L., Patel I., Jeffreys A. J. Rapid identification of donor and recipient cells after allogeneic bone marrow transplantation using specific genetic markers. Br J Haematol. 1989 Jun;72(2):133–140. doi: 10.1111/j.1365-2141.1989.tb07673.x. [DOI] [PubMed] [Google Scholar]
  34. Ironside J. W., Lewis F. A., Blythe D., Wakefield E. A. The identification of cells containing JC papovavirus DNA in progressive multifocal leukoencephalopathy by combined in situ hybridization and immunocytochemistry. J Pathol. 1989 Apr;157(4):291–297. doi: 10.1002/path.1711570405. [DOI] [PubMed] [Google Scholar]
  35. Jackson P., Lewis F. A., Wells M. In situ hybridization technique using an immunogold silver staining system. Histochem J. 1989 Jul;21(7):425–428. doi: 10.1007/BF01789741. [DOI] [PubMed] [Google Scholar]
  36. Jacob J., Todd K., Birnstiel M. L., Bird A. Molecular hybridization of 3H-labelled ribosomal RNA with DNA in ultrathin sections prepared for electron microscopy. Biochim Biophys Acta. 1971 Feb 11;228(3):761–766. doi: 10.1016/0005-2787(71)90746-5. [DOI] [PubMed] [Google Scholar]
  37. Jenkins E. C., Devine-Gage E. A., Yao X. L., Houck G. E., Jr, Brown W. T., Wisniewski H. M., Robakis N. K. In-situ hybridisation of the beta-amyloid protein probe to chromosome 9 in patients with familial Alzheimer's disease. Lancet. 1987 Nov 14;2(8568):1155–1156. doi: 10.1016/s0140-6736(87)91591-1. [DOI] [PubMed] [Google Scholar]
  38. John H. A., Birnstiel M. L., Jones K. W. RNA-DNA hybrids at the cytological level. Nature. 1969 Aug 9;223(5206):582–587. doi: 10.1038/223582a0. [DOI] [PubMed] [Google Scholar]
  39. Kennedy P. G., Newsome D. A., Hess J., Narayan O., Suresch D. L., Green W. R., Gallo R. C., Polk B. F. Cytomegalovirus but not human T lymphotropic virus type III/lymphadenopathy associated virus detected by in situ hybridisation in retinal lesions in patients with the acquired immune deficiency syndrome. Br Med J (Clin Res Ed) 1986 Jul 19;293(6540):162–164. doi: 10.1136/bmj.293.6540.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Langer P. R., Waldrop A. A., Ward D. C. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633–6637. doi: 10.1073/pnas.78.11.6633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Le Beau M. M., Diaz M. O., Karin M., Rowley J. D. Metallothionein gene cluster is split by chromosome 16 rearrangements in myelomonocytic leukaemia. Nature. 1985 Feb 21;313(6004):709–711. doi: 10.1038/313709a0. [DOI] [PubMed] [Google Scholar]
  42. Lewis M. E., Sherman T. G., Watson S. J. In situ hybridization histochemistry with synthetic oligonucleotides: strategies and methods. Peptides. 1985;6 (Suppl 2):75–87. doi: 10.1016/0196-9781(85)90138-x. [DOI] [PubMed] [Google Scholar]
  43. Lindeberg H., Fey S. J., Ottosen P. D., Mose Larsen P. Human papilloma virus (HPV) and carcinomas of the head and neck. Clin Otolaryngol Allied Sci. 1988 Dec;13(6):447–454. doi: 10.1111/j.1365-2273.1988.tb00318.x. [DOI] [PubMed] [Google Scholar]
  44. Lo C. W. Localization of low abundance DNA sequences in tissue sections by in situ hybridization. J Cell Sci. 1986 Mar;81:143–162. doi: 10.1242/jcs.81.1.143. [DOI] [PubMed] [Google Scholar]
  45. Lux S. E., Tse W. T., Menninger J. C., John K. M., Harris P., Shalev O., Chilcote R. R., Marchesi S. L., Watkins P. C., Bennett V. Hereditary spherocytosis associated with deletion of human erythrocyte ankyrin gene on chromosome 8. Nature. 1990 Jun 21;345(6277):736–739. doi: 10.1038/345736a0. [DOI] [PubMed] [Google Scholar]
  46. Macnab J. C., Walkinshaw S. A., Cordiner J. W., Clements J. B. Human papillomavirus in clinically and histologically normal tissue of patients with genital cancer. N Engl J Med. 1986 Oct 23;315(17):1052–1058. doi: 10.1056/NEJM198610233151703. [DOI] [PubMed] [Google Scholar]
  47. Matsumoto Y. Simultaneous inhibition of endogenous avidin-binding activity and peroxidase applicable for the avidin-biotin system using monoclonal antibodies. Histochemistry. 1985;83(4):325–330. doi: 10.1007/BF00684378. [DOI] [PubMed] [Google Scholar]
  48. McDougall J. K., Beckmann A. M., Kiviat N. B. Methods for diagnosing papillomavirus infection. Ciba Found Symp. 1986;120:86–103. doi: 10.1002/9780470513309.ch7. [DOI] [PubMed] [Google Scholar]
  49. McDougall J. K., Myerson D., Beckmann A. M. Detection of viral DNA and RNA by in situ hybridization. J Histochem Cytochem. 1986 Jan;34(1):33–38. doi: 10.1177/34.1.3001177. [DOI] [PubMed] [Google Scholar]
  50. McKeating J. A., Al-Nakib W., Greenaway P. J., Griffiths P. D. Detection of cytomegalovirus by DNA-DNA hybridization employing probes labelled with 32-phosphorus or biotin. J Virol Methods. 1985 Jul;11(3):207–216. doi: 10.1016/0166-0934(85)90109-0. [DOI] [PubMed] [Google Scholar]
  51. Naoumov N. V., Alexander G. J., Eddleston A. L., Williams R. In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes. J Clin Pathol. 1988 Jul;41(7):793–798. doi: 10.1136/jcp.41.7.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. Nelson J. A., Reynolds-Kohler C., Oldstone M. B., Wiley C. A. HIV and HCMV coinfect brain cells in patients with AIDS. Virology. 1988 Jul;165(1):286–290. doi: 10.1016/0042-6822(88)90685-x. [DOI] [PubMed] [Google Scholar]
  53. Obara T., Baba M., Yamaguchi Y., Fuchs E., Resau J. H., Trump B. F., Klein-Szanto A. J. Localization of keratin mRNA in human tracheobronchial epithelium and bronchogenic carcinomas by in situ hybridization. Am J Pathol. 1988 Jun;131(3):519–529. [PMC free article] [PubMed] [Google Scholar]
  54. Orth G., Jeanteur P., Croissant O. Evidence for and localization of vegetative viral DNA replication by autoradiographic detection of RNA-DNA hybrids in sections of tumors induced by Shope papilloma virus. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1876–1880. doi: 10.1073/pnas.68.8.1876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Pardue M. L., Gall J. G. Nucleic acid hybridization to the DNA of cytological preparations. Methods Cell Biol. 1975;10:1–16. doi: 10.1016/s0091-679x(08)60727-x. [DOI] [PubMed] [Google Scholar]
  56. Pezzella M., Pezzella F., Galli C., Macchi B., Verani P., Sorice F., Baroni C. D. In situ hybridization of human immunodeficiency virus (HTLV-III) in cryostat sections of lymph nodes of lymphadenopathy syndrome patients. J Med Virol. 1987 Jun;22(2):135–142. doi: 10.1002/jmv.1890220204. [DOI] [PubMed] [Google Scholar]
  57. Popescu N. C., DiPaolo J. A., Amsbaugh S. C. Integration sites of human papillomavirus 18 DNA sequences on HeLa cell chromosomes. Cytogenet Cell Genet. 1987;44(1):58–62. doi: 10.1159/000132342. [DOI] [PubMed] [Google Scholar]
  58. Pringle J. H., Homer C. E., Warford A., Kendall C. H., Lauder I. In situ hybridization: alkaline phosphatase visualization of biotinylated probes in cryostat and paraffin sections. Histochem J. 1987 Sep;19(9):488–496. doi: 10.1007/BF01675419. [DOI] [PubMed] [Google Scholar]
  59. Pringle J. H., Primrose L., Kind C. N., Talbot I. C., Lauder I. In situ hybridization demonstration of poly-adenylated RNA sequences in formalin-fixed paraffin sections using a biotinylated oligonucleotide poly d(T) probe. J Pathol. 1989 Aug;158(4):279–286. doi: 10.1002/path.1711580403. [DOI] [PubMed] [Google Scholar]
  60. Pringle J. H., Ruprai A. K., Primrose L., Keyte J., Potter L., Close P., Lauder I. In situ hybridization of immunoglobulin light chain mRNA in paraffin sections using biotinylated or hapten-labelled oligonucleotide probes. J Pathol. 1990 Nov;162(3):197–207. doi: 10.1002/path.1711620305. [DOI] [PubMed] [Google Scholar]
  61. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  62. Saber M. A., Zern M. A., Shafritz D. A. Use of in situ hybridization to identify collagen and albumin mRNAs in isolated mouse hepatocytes. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4017–4020. doi: 10.1073/pnas.80.13.4017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  63. Senior P. V., Critchley D. R., Beck F., Walker R. A., Varley J. M. The localization of laminin mRNA and protein in the postimplantation embryo and placenta of the mouse: an in situ hybridization and immunocytochemical study. Development. 1988 Nov;104(3):431–446. doi: 10.1242/dev.104.3.431. [DOI] [PubMed] [Google Scholar]
  64. Sequiera L. W., Jennings L. C., Carrasco L. H., Lord M. A., Curry A., Sutton R. N. Detection of herpes-simplex viral genome in brain tissue. Lancet. 1979 Sep 22;2(8143):609–612. doi: 10.1016/s0140-6736(79)91667-2. [DOI] [PubMed] [Google Scholar]
  65. Sixbey J. W., Nedrud J. G., Raab-Traub N., Hanes R. A., Pagano J. S. Epstein-Barr virus replication in oropharyngeal epithelial cells. N Engl J Med. 1984 May 10;310(19):1225–1230. doi: 10.1056/NEJM198405103101905. [DOI] [PubMed] [Google Scholar]
  66. Syrjänen S., Partanen P., Mäntyjärvi R., Syrjänen K. Sensitivity of in situ hybridization techniques using biotin- and 35S-labeled human papillomavirus (HPV) DNA probes. J Virol Methods. 1988 Mar-Apr;19(3-4):225–238. doi: 10.1016/0166-0934(88)90017-1. [DOI] [PubMed] [Google Scholar]
  67. Tase T., Okagaki T., Clark B. A., Manias D. A., Ostrow R. S., Twiggs L. B., Faras A. J. Human papillomavirus types and localization in adenocarcinoma and adenosquamous carcinoma of the uterine cervix: a study by in situ DNA hybridization. Cancer Res. 1988 Feb 15;48(4):993–998. [PubMed] [Google Scholar]
  68. Terry R. M., Lewis F. A., Griffiths S., Wells M., Bird C. C. Demonstration of human papillomavirus types 6 and 11 in juvenile laryngeal papillomatosis by in-situ DNA hybridization. J Pathol. 1987 Nov;153(3):245–248. doi: 10.1002/path.1711530308. [DOI] [PubMed] [Google Scholar]
  69. Unger E. R., Chandler F. W., Chenggis M. L., Ou C. Y., Warfield D. T., Feorino P. M. Demonstration of human immunodeficiency virus by colorimetric in situ hybridization: a rapid technique for formalin-fixed paraffin-embedded material. Mod Pathol. 1989 May;2(3):200–204. [PubMed] [Google Scholar]
  70. Van den Berg F., Schipper M., Jiwa M., Rook R., Van de Rijke F., Tigges B. Implausibility of an aetiological association between cytomegalovirus and Kaposi's sarcoma shown by four techniques. J Clin Pathol. 1989 Feb;42(2):128–131. doi: 10.1136/jcp.42.2.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  71. Van der Laarse W. J., Maslam S., Diegenbach P. C. Relationship between myoglobin and succinate dehydrogenase in mouse soleus and plantaris muscle fibres. Histochem J. 1985 Jan;17(1):1–11. doi: 10.1007/BF01003398. [DOI] [PubMed] [Google Scholar]
  72. Walker R. A., Senior P. V., Jones J. L., Critchley D. R., Varley J. M. An immunohistochemical and in situ hybridization study of c-myc and c-erbB-2 expression in primary human breast carcinomas. J Pathol. 1989 Jun;158(2):97–105. doi: 10.1002/path.1711580204. [DOI] [PubMed] [Google Scholar]
  73. Warford A. In situ hybridisation: a new tool in pathology. Med Lab Sci. 1988 Oct;45(4):381–394. [PubMed] [Google Scholar]
  74. Webster H. F., Lamperth L., Favilla J. T., Lemke G., Tesin D., Manuelidis L. Use of a biotinylated probe and in situ hybridization for light and electron microscopic localization of Po mRNA in myelin-forming Schwann cells. Histochemistry. 1987;86(5):441–444. doi: 10.1007/BF00500614. [DOI] [PubMed] [Google Scholar]
  75. Weiss L. M., Movahed L. A. In situ demonstration of Epstein-Barr viral genomes in viral-associated B cell lymphoproliferations. Am J Pathol. 1989 Mar;134(3):651–659. [PMC free article] [PubMed] [Google Scholar]
  76. Weiss L. M., Strickler J. G., Warnke R. A., Purtilo D. T., Sklar J. Epstein-Barr viral DNA in tissues of Hodgkin's disease. Am J Pathol. 1987 Oct;129(1):86–91. [PMC free article] [PubMed] [Google Scholar]
  77. Wood G. S., Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem. 1981 Oct;29(10):1196–1204. doi: 10.1177/29.10.7028859. [DOI] [PubMed] [Google Scholar]

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