Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1978 Jan 1;76(1):31–42. doi: 10.1083/jcb.76.1.31

Ultrastructural changes and cyclic AMP in frog oxyntic cells

PMCID: PMC2109961  PMID: 201646

Abstract

In vitro frog gastric mucosa was employed as a model for a combined physiological, biochemical, and ultrastructural study of the morphological changes which accompany the onset of acid secretion by the oxyntic cell. The histamine H2-receptor antagonist metiamide was used to provide a reproducible control state. Stimulation of acid production by theophylline resulted in a 10-fold increase in plasma membrane surface area and a distinct change in the conformation of mitochondrial cristae. Studies using the acid secretion inhibitors, thiocyanate and anoxia, demonstrated that neither acid production per se nor oxidative metabolism is essential for the theophylline-dependent changes in surface area. Increases in tissue cyclic AMP levels were observed under the conditions producing morphological changes. It is postulated that surface area changes induced by theophylline are controlled by cellular cyclic AMP levels.

Full Text

The Full Text of this article is available as a PDF (4.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black J. W., Duncan W. A., Durant C. J., Ganellin C. R., Parsons E. M. Definition and antagonism of histamine H 2 -receptors. Nature. 1972 Apr 21;236(5347):385–390. doi: 10.1038/236385a0. [DOI] [PubMed] [Google Scholar]
  2. Chew C. S., Rinard G. A. Estrogenic regulation of uterine cyclic AMP metabolism. Biochim Biophys Acta. 1974 Oct 8;362(3):493–500. doi: 10.1016/0304-4165(74)90144-5. [DOI] [PubMed] [Google Scholar]
  3. Forte T. M., Machen T. E., Forte J. G. Ultrastructural and physiological changes in piglet oxyntic cells during histamine stimulation and metabolic inhibition. Gastroenterology. 1975 Dec;69(6):1208–1222. [PubMed] [Google Scholar]
  4. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harris J. B., Nigon K., Alonso D. Adenosine-3',5'-monophosphate: intracellular mediator for methyl xanthine stimulation of gastric secretion. Gastroenterology. 1969 Oct;57(4):377–384. [PubMed] [Google Scholar]
  6. Helander H. F., Hirschowitz B. I. Quantitative ultrastructural studies on gastric parietal cells. Gastroenterology. 1972 Dec;63(6):951–961. [PubMed] [Google Scholar]
  7. Helander H. F., Hirschowitz B. I. Quantitative ultrastructural studies on inhibited and on partly stimulated gastric parietal cells. Gastroenterology. 1974 Sep;67(3):447–452. [PubMed] [Google Scholar]
  8. Hersey S. J. Interactions between oxidative metabolism and acid secretion in gastric mucosa. Biochim Biophys Acta. 1974 Sep 16;344(2):157–203. doi: 10.1016/0304-4157(74)90003-3. [DOI] [PubMed] [Google Scholar]
  9. High W. L., Hersey S. J. Mechanism of theophylline stimulation of acid secretion by frog gastric mucosa. Am J Physiol. 1974 Jun;226(6):1408–1412. doi: 10.1152/ajplegacy.1974.226.6.1408. [DOI] [PubMed] [Google Scholar]
  10. Ito S., Schofield G. C. Studies on the depletion and accumulation of microvilli and changes in the tubulovesicular compartment of mouse parietal cells in relation to gastric acid secretion. J Cell Biol. 1974 Nov;63(2 Pt 1):364–382. doi: 10.1083/jcb.63.2.364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kasbekar D. K., Forte G. M., Forte J. G. Phospholipid turnover and ultrastructural changes in resting and secreting bullfrog gastric mucosa. Biochim Biophys Acta. 1968 Aug;163(1):1–13. doi: 10.1016/0005-2736(68)90026-6. [DOI] [PubMed] [Google Scholar]
  12. Kasbekar D. K. Studies of resting isolated frog gastric mucosa. Proc Soc Exp Biol Med. 1967 May;125(1):267–271. doi: 10.3181/00379727-125-32066. [DOI] [PubMed] [Google Scholar]
  13. Koch A. L., Putnam S. L. Sensitive biuret method for determination of protein in an impure system such as whole bacteria. Anal Biochem. 1971 Nov;44(1):239–245. doi: 10.1016/0003-2697(71)90366-6. [DOI] [PubMed] [Google Scholar]
  14. Porter K. R., Puck T. T., Hsie A. W., Kelley D. An electron microscopy study of the effects on dibutyryl cyclic AMP on Chinese hamster ovary cells. Cell. 1974 Jul;2(3):145–162. doi: 10.1016/0092-8674(74)90089-0. [DOI] [PubMed] [Google Scholar]
  15. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SEDAR A. W. Electron microscopy of the oxyntic cell in the gastric glands of the bullfrog (Rana catesbiana). I. The non-acid-secreting gastric mucosa. J Biophys Biochem Cytol. 1961 Jan;9:1–18. doi: 10.1083/jcb.9.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SEDAR A. W. Electron microscopy of the oxyntic cell in the gastric glands of the bullfrog, Rana catesbiana. II. The acid-secreting gastric mucosa. J Biophys Biochem Cytol. 1961 May;10:47–57. doi: 10.1083/jcb.10.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sedar A. W. Fine structure of the stimulated oxyntic cell. Fed Proc. 1965 Nov-Dec;24(6):1360–1367. [PubMed] [Google Scholar]
  19. Sedar A. W., Wiebelhaus V. K + effects on acid secretion and ultrastructure of the amphibian oxyntic cell. Am J Physiol. 1972 Nov;223(5):1088–1092. doi: 10.1152/ajplegacy.1972.223.5.1088. [DOI] [PubMed] [Google Scholar]
  20. Shoemaker R. L., Buckner E., Spenney J. G., Sachs G. Action of Burimamide, a histamine antagonist, on acid secretion in vitro. Am J Physiol. 1974 Apr;226(4):898–902. doi: 10.1152/ajplegacy.1974.226.4.898. [DOI] [PubMed] [Google Scholar]
  21. WEIBEL E. R. Principles and methods for the morphometric study of the lung and other organs. Lab Invest. 1963 Feb;12:131–155. [PubMed] [Google Scholar]
  22. Willingham M. C., Pastan I. Cyclic AMP modulates microvillus formation and agglutinability in transformed and normal mouse fibroblasts. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1263–1267. doi: 10.1073/pnas.72.4.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Willingham M. C., Pastan I. Cyclic amp and cell morphology in cultured fibroblasts. Effects on cell shape, microfilament and microtubule distribution, and orientation to substratum. J Cell Biol. 1975 Oct;67(1):146–159. doi: 10.1083/jcb.67.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES