Abstract
When simian virus 40 DNA is extracted from infected cells with low concentrations of sodium deoxycholate, which selectively extract non-encapsidated simian virus 40 DNA, the DNA has a lower average number of superhelical turns than the DNA extracted from purified viral particles. During extraction, a partial deproteinization of the DNA by a concentration of detergent that did not inactivate a nicking-closing activity led to the removal of some superhelical turns. The DNA extracted in this way no longer reflected its in vivo number of superhelical turns.
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- Bellard M., Oudet P., Germond J. E., Chambon P. Subunit structure of simian-virus-40 minichromosome. Eur J Biochem. 1976 Nov 15;70(2):543–553. doi: 10.1111/j.1432-1033.1976.tb11046.x. [DOI] [PubMed] [Google Scholar]
- Bourgaux P., Bourgaux-Ramoisy D., Dulbecco R. The replication of the ring-shaped DNA of polyoma virus. I. Identification of the replicative intermediate. Proc Natl Acad Sci U S A. 1969 Oct;64(2):701–708. doi: 10.1073/pnas.64.2.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Champoux J. J., Dulbecco R. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay). Proc Natl Acad Sci U S A. 1972 Jan;69(1):143–146. doi: 10.1073/pnas.69.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Champoux J. J. Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3800–3804. doi: 10.1073/pnas.74.9.3800. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eason R., Vinograd J. Superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid. J Virol. 1971 Jan;7(1):1–7. doi: 10.1128/jvi.7.1.1-7.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Germond J. E., Bellard M., Oudet P., Chambon P. Stability of nucleosomes in native and reconstituted chromatins. Nucleic Acids Res. 1976 Nov;3(11):3173–3192. doi: 10.1093/nar/3.11.3173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Germond J. E., Hirt B., Oudet P., Gross-Bellark M., Chambon P. Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A. 1975 May;72(5):1843–1847. doi: 10.1073/pnas.72.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smart J. E., Bonner J. Selective dissociation of histones from chromatin by sodium deoxycholate. J Mol Biol. 1971 Jun 28;58(3):651–659. doi: 10.1016/0022-2836(71)90030-1. [DOI] [PubMed] [Google Scholar]