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. 1990 Aug;172(8):4715–4718. doi: 10.1128/jb.172.8.4715-4718.1990

Conservation of hydrogenase and polyferredoxin structures in the hyperthermophilic archaebacterium Methanothermus fervidus.

V J Steigerwald 1, G S Beckler 1, J N Reeve 1
PMCID: PMC213312  PMID: 2115877

Abstract

A 3.3-kilobase-pair region of the Methanothermus fervidus genome encoding part of the small subunit and all of the large subunit of the methyl viologen-reducing hydrogenase and a polyferredoxin was cloned and sequenced. The sequence of this hyperthermophilic hydrogenase conforms to the consensus sequence established for procaryotic [NiFe] hydrogenases. Although the M. fervidus polyferredoxin is the same size as the Methanobacterium thermoautotrophicum ferredoxin, containing six tandemly arranged bacterial ferredoxinlike domains, these two proteins are predicted to be only 64% identical in their primary sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eberz G., Hogrefe C., Kortlüke C., Kamienski A., Friedrich B. Molecular cloning of structural and regulatory hydrogenase (hox) genes of Alcaligenes eutrophus H16. J Bacteriol. 1986 Nov;168(2):636–641. doi: 10.1128/jb.168.2.636-641.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fabry S., Lang J., Niermann T., Vingron M., Hensel R. Nucleotide sequence of the glyceraldehyde-3-phosphate dehydrogenase gene from the mesophilic methanogenic archaebacteria Methanobacterium bryantii and Methanobacterium formicicum. Comparison with the respective gene structure of the closely related extreme thermophile Methanothermus fervidus. Eur J Biochem. 1989 Feb 1;179(2):405–413. doi: 10.1111/j.1432-1033.1989.tb14568.x. [DOI] [PubMed] [Google Scholar]
  3. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  4. Menon N. K., Robbins J., Peck H. D., Jr, Chatelus C. Y., Choi E. S., Przybyla A. E. Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames. J Bacteriol. 1990 Apr;172(4):1969–1977. doi: 10.1128/jb.172.4.1969-1977.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Menéndez-Arias L., Argos P. Engineering protein thermal stability. Sequence statistics point to residue substitutions in alpha-helices. J Mol Biol. 1989 Mar 20;206(2):397–406. doi: 10.1016/0022-2836(89)90488-9. [DOI] [PubMed] [Google Scholar]
  6. Reeve J. N., Beckler G. S., Cram D. S., Hamilton P. T., Brown J. W., Krzycki J. A., Kolodziej A. F., Alex L., Orme-Johnson W. H., Walsh C. T. A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain delta H encodes a polyferredoxin. Proc Natl Acad Sci U S A. 1989 May;86(9):3031–3035. doi: 10.1073/pnas.86.9.3031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Weil C. F., Cram D. S., Sherf B. A., Reeve J. N. Structure and comparative analysis of the genes encoding component C of methyl coenzyme M reductase in the extremely thermophilic archaebacterium Methanothermus fervidus. J Bacteriol. 1988 Oct;170(10):4718–4726. doi: 10.1128/jb.170.10.4718-4726.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Zillig W., Palm P., Reiter W. D., Gropp F., Pühler G., Klenk H. P. Comparative evaluation of gene expression in archaebacteria. Eur J Biochem. 1988 May 2;173(3):473–482. doi: 10.1111/j.1432-1033.1988.tb14023.x. [DOI] [PubMed] [Google Scholar]

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