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. 1990 Apr;172(4):2181–2183. doi: 10.1128/jb.172.4.2181-2183.1990

Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene.

R D Cardin 1, A J Biel 1
PMCID: PMC208721  PMID: 2156812

Abstract

We isolated a Tn5 insertion mutant of Rhodobacter capsulatus which requires biotin for growth. Crossfeeding studies with Escherichia coli bio mutants indicated that the insertion is in the bioB gene. A cosmid that complements the bioB insertion was isolated, and the bioB gene was localized to a 2.85-kilobase EcoRI-PstI fragment.

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Selected References

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