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. 1990 Apr;136(4):899–908.

Variable expression of desmin in rat glomerular epithelial cells.

E Yaoita 1, K Kawasaki 1, T Yamamoto 1, I Kihara 1
PMCID: PMC1877632  PMID: 2183627

Abstract

The presence of desmin, regarded as a marker of myogenic cells, in glomerular epithelial cells (GECs) of the kidney was studied in four strains of rat (SHR, Lewis, Fischer 344, WKA) by immunofluorescence microscopy and immunoelectron microscopy. Some of the GECs were stained with both monoclonal and polyclonal anti-desmin antibodies. The frequency of desmin-positive cells among GECs varied between glomeruli in each of the strains and also differed between the four strains. Most GECs in WKA rats contained desmin, whereas few did so in SHR rats. Lewis and Fischer 344 rats showed amounts of GEC desmin staining intermediate between those of SHR and WKA rats. In every strain, desmin-specific immunofluorescence in GECs increased with aging. In aminonucleoside nephrosis, GECs showed extremely enhanced desmin staining in parallel with urinary protein excretion. This heterogeneous distribution and changes observed for desmin were not shown for vimentin. On the basis of the above findings, the correlation between the presence of desmin in GECs and glomerular epithelial damage is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann S., Kriz W., Kuhn C., Franke W. W. Differentiation of cell types in the mammalian kidney by immunofluorescence microscopy using antibodies to intermediate filament proteins and desmoplakins. Histochemistry. 1983;77(3):365–394. doi: 10.1007/BF00490899. [DOI] [PubMed] [Google Scholar]
  2. Blose S. H., Bushnell A. Observations on the vimentin-10-NM filaments during mitosis in BHK21 cells. Exp Cell Res. 1982 Nov;142(1):57–62. doi: 10.1016/0014-4827(82)90408-6. [DOI] [PubMed] [Google Scholar]
  3. Brenner B. M., Troy J. L., Daugharty T. M. The dynamics of glomerular ultrafiltration in the rat. J Clin Invest. 1971 Aug;50(8):1776–1780. doi: 10.1172/JCI106667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cordon-Cardo C., Finstad C. L., Bander N. H., Melamed M. R. Immunoanatomic distribution of cytostructural and tissue-associated antigens in the human urinary tract. Am J Pathol. 1987 Feb;126(2):269–284. [PMC free article] [PubMed] [Google Scholar]
  5. Dale B. A., Holbrook K. A., Kimball J. R., Hoff M., Sun T. T. Expression of epidermal keratins and filaggrin during human fetal skin development. J Cell Biol. 1985 Oct;101(4):1257–1269. doi: 10.1083/jcb.101.4.1257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies P. F., Remuzzi A., Gordon E. J., Dewey C. F., Jr, Gimbrone M. A., Jr Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2114–2117. doi: 10.1073/pnas.83.7.2114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Drenckhahn D., Franke R. P. Ultrastructural organization of contractile and cytoskeletal proteins in glomerular podocytes of chicken, rat, and man. Lab Invest. 1988 Nov;59(5):673–682. [PubMed] [Google Scholar]
  8. Evans R. M., Fink L. M. An alteration in the phosphorylation of vimentin-type intermediate filaments is associated with mitosis in cultured mammalian cells. Cell. 1982 May;29(1):43–52. doi: 10.1016/0092-8674(82)90088-5. [DOI] [PubMed] [Google Scholar]
  9. FARQUHAR M. G., PALADE G. E. Glomerular permeability. II. Ferritin transfer across the glomerular capillary wall in nephrotic rats. J Exp Med. 1961 Nov 1;114:699–716. doi: 10.1084/jem.114.5.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fishman J. A., Karnovsky M. J. Effects of the aminonucleoside of puromycin on glomerular epithelial cells in vitro. Am J Pathol. 1985 Mar;118(3):398–407. [PMC free article] [PubMed] [Google Scholar]
  11. Franke W. W., Schmid E., Grund C., Geiger B. Intermediate filament proteins in nonfilamentous structures: transient disintegration and inclusion of subunit proteins in granular aggregates. Cell. 1982 Aug;30(1):103–113. doi: 10.1016/0092-8674(82)90016-2. [DOI] [PubMed] [Google Scholar]
  12. Franke W. W., Schmid E., Schiller D. L., Winter S., Jarasch E. D., Moll R., Denk H., Jackson B. W., Illmensee K. Differentiation-related patterns of expression of proteins of intermediate-size filaments in tissues and cultured cells. Cold Spring Harb Symp Quant Biol. 1982;46(Pt 1):431–453. doi: 10.1101/sqb.1982.046.01.041. [DOI] [PubMed] [Google Scholar]
  13. Fries J. W., Sandstrom D. J., Meyer T. W., Rennke H. G. Glomerular hypertrophy and epithelial cell injury modulate progressive glomerulosclerosis in the rat. Lab Invest. 1989 Feb;60(2):205–218. [PubMed] [Google Scholar]
  14. Fujimoto T., Singer S. J. Immunocytochemical studies of endothelial cells in vivo. I. The presence of desmin only, or of desmin plus vimentin, or vimentin only, in the endothelial cells of different capillaries of the adult chicken. J Cell Biol. 1986 Dec;103(6 Pt 2):2775–2786. doi: 10.1083/jcb.103.6.2775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Glasser S. R., Julian J. Intermediate filament protein as a marker of uterine stromal cell decidualization. Biol Reprod. 1986 Sep;35(2):463–474. doi: 10.1095/biolreprod35.2.463. [DOI] [PubMed] [Google Scholar]
  16. Holthöfer H., Miettinen A., Lehto V. P., Lehtonen E., Virtanen I. Expression of vimentin and cytokeratin types of intermediate filament proteins in developing and adult human kidneys. Lab Invest. 1984 May;50(5):552–559. [PubMed] [Google Scholar]
  17. Holthöfer H., Miettinen A., Paasivuo R., Lehto V. P., Linder E., Alfthan O., Virtanen I. Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, antiintermediate filament antibodies, and lectins. Lab Invest. 1983 Sep;49(3):317–326. [PubMed] [Google Scholar]
  18. Kanwar Y. S., Rosenzweig L. J. Altered glomerular permeability as a result of focal detachment of the visceral epithelium. Kidney Int. 1982 Apr;21(4):565–574. doi: 10.1038/ki.1982.63. [DOI] [PubMed] [Google Scholar]
  19. Lazarides E. Intermediate filaments as mechanical integrators of cellular space. Nature. 1980 Jan 17;283(5744):249–256. doi: 10.1038/283249a0. [DOI] [PubMed] [Google Scholar]
  20. Lazarides E. Intermediate filaments: a chemically heterogeneous, developmentally regulated class of proteins. Annu Rev Biochem. 1982;51:219–250. doi: 10.1146/annurev.bi.51.070182.001251. [DOI] [PubMed] [Google Scholar]
  21. McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem. 1974 Dec;22(12):1077–1083. doi: 10.1177/22.12.1077. [DOI] [PubMed] [Google Scholar]
  22. Michael A. F., Blau E., Vernier R. L. Glomerular polyanion. Alteration in aminonucleoside nephrosis. Lab Invest. 1970 Dec;23(6):649–657. [PubMed] [Google Scholar]
  23. Miyazaki S., Kawasaki K., Yaoita E., Yamamoto T., Kihara I. Bovine serum albumin nephritis in rats. V. Kinetic studies of antigen localization in various organs and the phagocytic role of polymorphonuclear leukocytes. Am J Pathol. 1985 Jun;119(3):412–419. [PMC free article] [PubMed] [Google Scholar]
  24. Pabst R., Sterzel R. B. Cell renewal of glomerular cell types in normal rats. An autoradiographic analysis. Kidney Int. 1983 Nov;24(5):626–631. doi: 10.1038/ki.1983.203. [DOI] [PubMed] [Google Scholar]
  25. Ryan G. B., Karnovsky M. J. An ultrastructural study of the mechanisms of proteinuria in aminonucleoside nephrosis. Kidney Int. 1975 Oct;8(4):219–232. doi: 10.1038/ki.1975.105. [DOI] [PubMed] [Google Scholar]
  26. Saxén L., Sariola H., Lehtonen E. Sequential cell and tissue interactions governing organogenesis of the kidney. Anat Embryol (Berl) 1986;175(1):1–6. doi: 10.1007/BF00315450. [DOI] [PubMed] [Google Scholar]
  27. Schmid E., Osborn M., Rungger-Brändle E., Gabbiani G., Weber K., Franke W. W. Distribution of vimentin and desmin filaments in smooth muscle tissue of mammalian and avian aorta. Exp Cell Res. 1982 Feb;137(2):329–340. doi: 10.1016/0014-4827(82)90034-9. [DOI] [PubMed] [Google Scholar]
  28. Shea S. M., Morrison A. B. A stereological study of the glomerular filter in the rat. Morphometry of the slit diaphragm and basement membrane. J Cell Biol. 1975 Nov;67(2PT1):436–443. doi: 10.1083/jcb.67.2.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shimizu F., Fukui M., Hamajima K., Murata M. Application of double staining technique and incident light fluorescence microscopy to immunofluorescence studies on renal diseases. Jpn J Exp Med. 1975 Feb;45(1):25–31. [PubMed] [Google Scholar]
  30. Skalli O., Ropraz P., Trzeciak A., Benzonana G., Gillessen D., Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986 Dec;103(6 Pt 2):2787–2796. doi: 10.1083/jcb.103.6.2787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stamenkovic I., Skalli O., Gabbiani G. Distribution of intermediate filament proteins in normal and diseased human glomeruli. Am J Pathol. 1986 Dec;125(3):465–475. [PMC free article] [PubMed] [Google Scholar]
  32. Steinert P. M., Idler W. W., Cabral F., Gottesman M. M., Goldman R. D. In vitro assembly of homopolymer and copolymer filaments from intermediate filament subunits of muscle and fibroblastic cells. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3692–3696. doi: 10.1073/pnas.78.6.3692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sun T. T., Shih C., Green H. Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2813–2817. doi: 10.1073/pnas.76.6.2813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Toccanier-Pelte M. F., Skalli O., Kapanci Y., Gabbiani G. Characterization of stromal cells with myoid features in lymph nodes and spleen in normal and pathologic conditions. Am J Pathol. 1987 Oct;129(1):109–118. [PMC free article] [PubMed] [Google Scholar]
  35. Travo P., Weber K., Osborn M. Co-existence of vimentin and desmin type intermediate filaments in a subpopulation of adult rat vascular smooth muscle cells growing in primary culture. Exp Cell Res. 1982 May;139(1):87–94. doi: 10.1016/0014-4827(82)90321-4. [DOI] [PubMed] [Google Scholar]
  36. Tölle H. G., Weber K., Osborn M. Microinjection of monoclonal antibodies to vimentin, desmin, and GFA in cells which contain more than one IF type. Exp Cell Res. 1986 Feb;162(2):462–474. doi: 10.1016/0014-4827(86)90350-2. [DOI] [PubMed] [Google Scholar]
  37. VERNIER R. L., PAPERMASTER B. W., GOOD R. A. Aminonucleoside nephrosis. I. Electron microscopic study of the renal lesion in rats. J Exp Med. 1959 Jan 1;109(1):115–126. doi: 10.1084/jem.109.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Venkatachalam M. A., Karnovsky M. J., Cotran R. S. Glomerular permeability. Ultrastructural studies in experimental nephrosis using horseradish peroxidase as a tracer. J Exp Med. 1969 Aug 1;130(2):381–399. doi: 10.1084/jem.130.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Yaoita E., Kazama T., Kawasaki K., Miyazaki S., Yamamoto T., Kihara I. In vitro characteristics of rat mesangial cells in comparison with aortic smooth muscle cells and dermal fibroblasts. Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(4):285–294. doi: 10.1007/BF02912106. [DOI] [PubMed] [Google Scholar]
  40. Yokoi Y., Namihisa T., Kuroda H., Komatsu I., Miyazaki A., Watanabe S., Usui K. Immunocytochemical detection of desmin in fat-storing cells (Ito cells). Hepatology. 1984 Jul-Aug;4(4):709–714. doi: 10.1002/hep.1840040425. [DOI] [PubMed] [Google Scholar]

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