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. 2011 Sep 29;27(5):300. doi: 10.1007/s12264-011-1034-4

Noradrenaline regulates substance P release from rat dorsal root ganglion neurons in vitro

去甲肾上腺素对背根神经节神经元 P 物质释放的调节作用

Yan-Jie Wang 1, Xing-Fu Li 1, Feng Ding 1, Qiang Shu 1, Li-Jun Song 1, Xiao Yu 1, Hua-Xiang Liu 1,✉
PMCID: PMC5560312  PMID: 21934725

Abstract

Objective

To determine whether activation and/or inhibition of α-adrenoreceptors influences substance P (SP) release from dorsal root ganglion (DRG) primary sensory neurons in vitro.

Methods

DRGs were dissected from 15-day embryonic Wistar rats. DRG neurons were dissociated and cultured for 2 d and then exposed to noradrenaline (NA) alone (1×10−4 mol/L), or along with the α1-adrenoreceptor antagonist prazosin (1×10−6 mol/L) or the α2-adrenoreceptor antagonist yohimbine (1×10−5 mol/L) for 4 d. Then, RT-PCR was used to determine the levels of preprotachykinin (PPT) mRNA encoding for SP and Western blot to assess the protein levels of SP. Basal and capsaicin (CAP)-evoked SP release were measured by enzyme-linked immunosorbent assay (ELISA).

Results

CAP-evoked SP release was sensitized by NA and this effect was inhibited by pre-incubation with prazosin but not with yohimbine. The levels of PPT mRNA, SP peptide, and basal SP release did not change significantly in any of the experimental conditions.

Conclusion

NA may significantly increase CAP-evoked SP release through activation of α-adrenoreceptors, which may contribute to noradrenergic pain modulation.

Keywords: noradrenaline, substance P, dorsal root ganglion

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