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. 1979 Jul;139(1):71–79. doi: 10.1128/jb.139.1.71-79.1979

Genetics and regulation of D-xylose utilization in Salmonella typhimurium LT2.

D K Shamanna, K E Sanderson
PMCID: PMC216828  PMID: 222732

Abstract

Twenty-one Xyl- mutants of Salmonella typhimurium were selected: all had lost one or more of the activities for D-xylose isomerase, C-xylulokinase, or D-xylose transport. The mutants were classified into five functional groups: xylR, pleiotropic negative (12 mutants); xylA, D-xylose isomerase defective (3 mutants); xylB, D-xylulokinase defective (2 mutants); xylT, D-xylose transport defective (1 mutant); and 3 mutants with defective D-xylose isomerase and D-xylulokinase. Some nonsense mutations were identified among the xylR mutants. Two F'xyl plasmids were isolated by selection for early transfer of xyl+ by an Hfr which transfers xyl as a terminal gene; a plasmid with a mutation in the xyl genes, F'xylR1, was also isolated. Complementation tests using F'xyl plasmids indicate that expression of the xylA, xylB, and xylT genes is under the positive control of the xylR regulatory gene. Conjugation crosses and P22-mediated transduction data indicate that all the xyl mutations tested are in a cluster of genes at 78 units on the linkage map, and that the gene order is xylT--xylR--xylB--xylA--glyS--mtlA,D.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abou-Sabé M. A., Ratner P. L. Genetic regulation of a constitutive operon in E. coli B-r. Biochem Biophys Res Commun. 1973 Dec 10;55(3):1015–1020. doi: 10.1016/0006-291x(73)91243-6. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beckwith J., Rossow P. Analysis of genetic regulatory mechanisms. Annu Rev Genet. 1974;8:1–13. doi: 10.1146/annurev.ge.08.120174.000245. [DOI] [PubMed] [Google Scholar]
  4. Berkowitz D., Hushon J. M., Whitfield H. J., Jr, Roth J., Ames B. N. Procedure for identifying nonsense mutations. J Bacteriol. 1968 Jul;96(1):215–220. doi: 10.1128/jb.96.1.215-220.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
  6. David J. D., Wiesmeyer H. Control of xylose metabolism in Escherichia coli. Biochim Biophys Acta. 1970 Mar 24;201(3):497–499. doi: 10.1016/0304-4165(70)90171-6. [DOI] [PubMed] [Google Scholar]
  7. David J., Wiesmeyer H. Regulation of ribose metabolism in Escherichia coli. I. The ribose catabolic pathway. Biochim Biophys Acta. 1970 Apr 14;208(1):45–55. doi: 10.1016/0304-4165(70)90047-4. [DOI] [PubMed] [Google Scholar]
  8. Englesberg E., Wilcox G. Regulation: positive control. Annu Rev Genet. 1974;8:219–242. doi: 10.1146/annurev.ge.08.120174.001251. [DOI] [PubMed] [Google Scholar]
  9. Ganem D., Miller J. H., Files J. G., Platt T., Weber K. Reinitiation of a lac repressor fragment at a codon other than AUG. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3165–3169. doi: 10.1073/pnas.70.11.3165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartman P. E. Some improved methods in P22 transduction. Genetics. 1974 Apr;76(4):625–631. doi: 10.1093/genetics/76.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hatfield D., Hofnung M., Schwartz M. Genetic analysis of the maltose A region in Escherichia coli. J Bacteriol. 1969 May;98(2):559–567. doi: 10.1128/jb.98.2.559-567.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hofnung M. Divergent operons and the genetic structure of the maltose B region in Escherichia coli K12. Genetics. 1974 Feb;76(2):169–184. doi: 10.1093/genetics/76.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hong J. S., Ames B. N. Localized mutagenesis of any specific small region of the bacterial chromosome. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158–3162. doi: 10.1073/pnas.68.12.3158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  15. Parks J. S., Gottesman M., Shimada K., Weisberg R. A., Perlman R. L., Pastan I. Isolation of the gal repressor. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1891–1895. doi: 10.1073/pnas.68.8.1891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Power J. The L-rhamnose genetic system in Escherichia coli K-12. Genetics. 1967 Mar;55(3):557–568. doi: 10.1093/genetics/55.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sanderson K. E., Hartman P. E. Linkage map of Salmonella typhimurium, edition V. Microbiol Rev. 1978 Jun;42(2):471–519. doi: 10.1128/mr.42.2.471-519.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanderson K. E., Ross H., Ziegler L., Mäkelä P. H. F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony. Bacteriol Rev. 1972 Dec;36(4):608–637. doi: 10.1128/br.36.4.608-637.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanderson K. E., Saeed H. Insertion of the F factor into the cluster of rfa (rough A) genes of Salmonella typhimurium. J Bacteriol. 1972 Oct;112(1):64–73. doi: 10.1128/jb.112.1.64-73.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanderson K. E., Saeed Y. A. P22-mediated transduction analysis of the rough A (rfa) region of the chromosome of Salmonella typhimurium. J Bacteriol. 1972 Oct;112(1):58–63. doi: 10.1128/jb.112.1.58-63.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Savageau M. A. Genetic regulatory mechanisms and the ecological niche of Escherichia coli. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2453–2455. doi: 10.1073/pnas.71.6.2453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shamanna D. K., Sanderson K. E. Uptake and catabolism of D-xylose in Salmonella typhimurium LT2. J Bacteriol. 1979 Jul;139(1):64–70. doi: 10.1128/jb.139.1.64-70.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith G. R., Magasanik B. Nature and self-regulated synthesis of the repressor of the hut operons in Salmonella typhimurium. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1493–1497. doi: 10.1073/pnas.68.7.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Straus D. S., D'Ari Straus L. Large overlapping tandem genetic duplications in Salmonella typhimurium. J Mol Biol. 1976 May 5;103(1):143–153. doi: 10.1016/0022-2836(76)90056-5. [DOI] [PubMed] [Google Scholar]

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